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SSR and STS markers for wheat stripe rust resistance gene Yr26

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Abstract

Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating wheat diseases worldwide. Triticum aestivum-Haynaldia villosa 6VS/6AL translocation lines carrying the Yr26 gene on chromosome 1B, are resistant to most races of Pst used in virulence tests. In order to better utilize Yr26 for wheat improvement, we attempted to screen SSR and EST-based STS markers closely linked with Yr26. A total of 500 F2 plants and the F2:3 progenies derived from a cross between 92R137 and susceptible cultivar Yangmai 5 were inoculated with race CYR32. The analysis confirmed that stripe rust resistance was controlled by a single dominant gene, Yr26. Among 35 pairs of genomic SSR markers and 81 pairs of STS markers derived from EST sequences located on chromosome 1B, Yr26 was flanked by 5 SSR and 7 STS markers. The markers were mapped in deletion bins using CS aneuploid and deletion lines. The closest flanking marker loci, Xwe173 and Xbarc181, mapped in 1BL and the genetic distances from Yr26 were 1.4 cM and 6.7 cM, respectively. Some of these markers were previously reported on 1BS. Eight common wheat cultivars and lines developed from the T. aestivum-H. villosa 6VS/6AL translocation lines by different research groups were tested for presence of the markers. Five lines with Yr26 carried the flanking markers whereas three lines without Yr26 did not. The results indicated that the flanking markers should be useful in marker-assisted selection for incorporating Yr26 into wheat cultivars.

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Acknowledgements

We are grateful to Prof. R.A. McIntosh for his critical review of this manuscript and Xiue Wang, Shouzhong Zhang, Baotong Wang and Shoucheng Chai for help in the study. This research was supported by grants from the National High Technology Research and Development Program of China (2001AA222152, 2004AA222140), the Natural Science Foundation of China (30471080), and the Program for Changjiang Scholars and Establishing Innovative Research Teams in Universities.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China

    Chunmei Wang, Yiping Zhang, Guiping Li, Aizhong Cao & Peidu Chen

  2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, 712100, China

    Dejun Han & Zhensheng Kang

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  1. Chunmei Wang
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  2. Yiping Zhang
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  3. Dejun Han
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  4. Zhensheng Kang
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  5. Guiping Li
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  6. Aizhong Cao
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  7. Peidu Chen
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Correspondence to Peidu Chen.

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Wang, C., Zhang, Y., Han, D. et al. SSR and STS markers for wheat stripe rust resistance gene Yr26 . Euphytica 159, 359–366 (2008). https://doi.org/10.1007/s10681-007-9524-1

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