Abstract
Glucosinolate levels in leaves were determined in a collection of 36 varieties of nabicol (Brassica napus pabularia group) from northwestern Spain grown at two locations. Crude protein, acid detergent fibre, and sensory traits were also assessed by a consumer panel. The objectives were to determine the diversity among varieties in total glucosinolate content and glucosinolate profile and to evaluate their sensory attributes in relation to glucosinolate content for breeding purposes. Eight glucosinolates were identified, being the aliphatic glucosinolates, glucobrassicanapin, progoitrin, and gluconapin the most abundant. Glucosinolate composition varied between locations although the glucosinolate pattern was not significantly influenced. Differences in total glucosinolate content, glucosinolate profile, protein, acid detergent fibre, and flavour were found among varieties. The total glucosinolate content ranged from 1.4 μmol g−1 to 41.0 μmol g−1 dw at one location and from 1.2 μmol g−1 to 7.6 μmol g−1 dw at the other location. Sensory analysis comparing bitterness and flavour with variation in glucosinolate, gluconapin, progoitrin, and glucobrassicanapin concentrations suggested that other phytochemicals are probably involved on the characteristic flavour. The variety MBG-BRS0035 had high total glucosinolate, glucobrassicanapin, and gluconapin contents at both locations and could be included in breeding programs to improve the nutritional value of this vegetable crop.
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References
AOAC (2000) Association of official analytical chemists. Official Methods of Analysis. AOAC International 17th edition, Arlington, VA
Carlson DG, Daxenbichler ME, Van Etten CH, Tookey HL, Williams PH (1981) Glucosinolates in crucifer vegetables: turnips and rutabagas. J Agric Food Chem 29:1235–1239
Cartea ME, Soengas P, Picoaga A, Ordás A (2005) Relationships among Brassica napus (L.) germplasm from Spain and Great Britain as determined by RAPD markers. Genet Resour Crop Evol 52:655–662
De Haro A, Fernández G, Baladrón JJ, Ordás A (1995) Estudio de la variabilidad respecto a componentes nutritivos en brassicas gallegas. Proceedings of VI Congreso de la Sociedad Española de Ciencias Hortícolas, Barcelona, Spain, p. 123
Farnham MW, Wilson PE, Stephenson KK, Fahey JW (2004) Genetic and environmental effects on glucosinolate content and chemoprotective potency of broccoli. Plant Breed 123:60–65
Fenwick RG, Griffiths NM, Heaney RK (1983a) Bitterness in Brussels sprouts (Brassica oleracea L. var. gemmifera): the role of glucosinolates and their breakdown products. J Sci Food Agric 34:73–80
Fenwick RG, Heaney RK, Mullin WJ (1983b) Glucosinolates and their breakdown products in food plants. CRC Crit Rev Food Sci Nutr 18:123–201
Font R, del Río M, Cartea ME, de Haro A (2005) Application of Near-Infrared Spectroscopy to the analysis of total and individual glucosinolates in Brassica napus leaves. Phytochemistry 66:175–185
Giamoustaris A, Mithen R (1996) Genetic of aliphatic glucosinolates. Side-chain modification in Brassica oleracea. Theor Appl Genet 93:1006–1010
Griffiths DH, Bradshaw JE, Taylor J, Gemmell DJ (1991) Effect of cultivar and harvest date on the glucosinolates and S-methylcystein sulphoxide content on sweedes (Brassica napus spp. rapifera). J Sci Food Agric 56:539–549
ISO Norm (1992) Rapeseed–determination of glucosinolates-part 1: method using high-performance liquid chromatography. ISO 9167-1, pp 1–9
Johnson HW, Robinson HF, Comstock RE (1955) Genotypic and phenotypic correlations in soybeans and their implications in selection. Agron J 47:477–483
Jones G, Sanders OG (2002) A sensory profile of turnip greens as affected by variety and maturity. J Food Sci 67:3126–3129
Li G, Quiros CF (2003) In planta side-chain glucosinolate modification in Arabidopsis by introduction of dioxygenase Brassica homolog BoGSL-ALK. Theor Appl Genet 106:1116–1121
Li Y, Kiddle G, Bennet R, Doughty K, Wallsgrove R (1999) Variation in the glucosinolate content of vegetative tissues of Chinese lines of Brassica napus L. Ann Appl Biol 134:131–136
Mithen R, Faulkner K, Magrath R, Rose P, Williamson G, Marquez J (2003) Development of isothiocyanate-enriched broccoli and its enhanced ability to induce phase 2 detoxification enzymes in mammalian cells. Theor Appl Genet 106:727–734
Mithen RF, Dekker M, Verkerk R, Rabot S, Johnson IT (2000) The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods. J Sci Food Agric 80:967–984
Padilla G, Cartea ME, Velasco P, de Haw A, Ordas A (2007) Variation of glucosinolates in vegetable crops of Brassica rapa. Phytochemistry 68:536–545
Picoaga A, Cartea ME, Soengas P, Monetti L, Ordás A (2003) Resistance of kale populations to Lepidopterous pests in northwestern Spain. J Econ Entomol 96:143–147
Rodríguez VM, Cartea ME, Padilla G, Velasco P, Ordás A (2005) The nabicol: a horticultural crop in northwestern Spain. Euphytica 142:237–246
Rosa EAS (1999) Chemical composition. In: Gómez-Campo C (ed) Biology of Brassica coenospecies. Elsevier Science BV, Amsterdam, pp 315–357
Rosa EAS, Heaney RK (1996) Seasonal variation in protein, mineral, and glucosinolates composition of Portuguese Brassica crops. Anim Feed Sci Technol 57: 111–127
Rosa EAS, Heaney RK, Fenwick GR, Portas CAM (1997) Glucosinolates in crop plants. Hort Rev 19:99–215
Rosa EAS, Heaney RK, Portas CAM, Fenwick GR (1996) Changes in glucosinolate concentrations in Brassica crops (B. oleracea and B. napus) throughout growing seasons. J Sci Food Agric 71:237–244
Rose P, Huang Q, Ong CN, Whiteman M (2005) Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells. Toxicol Appl Pharmacol 209:105–113
SAS Institute Inc. (2000) SAS OnlineDoc, version 8. SAS Institute. Inc., Cary, North Carolina, USA
Schonhof I, Krumbein A, Brückner B (2004) Genotypic effects on glucosinolates and sensory properties of broccoli and cauliflower. Nahrung/Food 48:25–33
Smith TK, Lund EK, Clarke RG, Bennet RN, Johnson IT (2005) Effects of Brussels sprout juice on the cell cycle and adhesion of human colorectal carcinoma cells (HT29) in vitro. J Agric Food Chem 53:3895–3901
Soengas P, Velasco P, Padilla G, Ordás A, Cartea ME (2006) Genetic relationships among Brassica napus crops based on SSRs markers. HortScience 41:1195–1199
Steel RDG, Torrie JH, Dickey DA (1997) Principles and procedures in statistics: a biometrical approach, 3rd ed. Mc Graw Hill, New York, USA
Van Doorn HE, Van der Kruk GC, Van Holst GJ, Raaijmakers-Ruijs NC, Potsma E, Groeneweg B, Jongen WHF (1998) The glucosinolates sinigrin and progoitrin are important determinants for taste preference and bitterness of Brussels sprouts. J Sci Food Agric 78:30–38
Wiedenhoeft MH, Barton BA (1994) Management and environmental effects on Brassica forage quality. Agron J 86:227–232
Acknowledgements
We thank G. Fernández, E. Santiago and R. Abilleira for work laboratory. This work has been supported by the Projects AGL 2003-01366 and AGL 2006-04055 of the Spanish Government and Excma. Diputaciόn Provincial De Pontevedra. V.M. Rodríguez acknowledges a fellowship from the Ministry of Science and Technology from Spain.
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Cartea, M.E., Rodríguez, V.M., de Haro, A. et al. Variation of glucosinolates and nutritional value in nabicol (Brassica napus pabularia group). Euphytica 159, 111–122 (2008). https://doi.org/10.1007/s10681-007-9463-x
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DOI: https://doi.org/10.1007/s10681-007-9463-x