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The genus Dasypyrum––part 1. The taxonomy and relationships within Dasypyrum and with Triticeae species

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Abstract

The article is an attempt to summarize available information about the relationships within Dasypyrum (Triticeae, Poaceae) and between Dasypyrum and other genera in the Triticeae. A relationship between the annual diploid D. villosum (L.) P. Candargy and the perennial tetraploid D. breviaristatum (L.) Frederiksen is discussed on the basis of cytological and molecular investigations. Although the relationship between the two Dasypyrum species seems to be quite close, D. breviaristatum is an autotetraploid with a basic genome different from that of D. villosum. The position of Dasypyrum in relation to other Triticeae is summarized, with special attention being paid to the Triticum and Secale. The closer relationship of Dasypyrum with Secale in comparison to Triticum seems to be reliable, even if only few intergeneric hybrids have been produced. It may be related to the fact that only a few experiments on production of those hybrids have been conducted till the present day, in contrast to experiments concerning hybrids with Triticum.

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References

  • Antonacci M, Urbano M, Blanco A (1994) Isolation and characterization of Triticum turgidum L./Dasypyrum villosum (L.) Candargy monosomic addition line. Annali della Facolta di Agraria, Universita di Bari 1993 34:95–99

    Google Scholar 

  • Apolinarska B, Gruszecka D (2001) Transfer genów z Dasypyrum villosum (Haynaldia villosa L.) do Secale cereale L. Biotechnologia 2(53):63–65

    Google Scholar 

  • Baum BR (1977) Taxonomy of the tribe Triticeae (Poaceae) using various numerical techniques I. Can J Bot 55:1712

    Article  Google Scholar 

  • Baum BR (1978a) Taxonomy of the tribe Triticeae (Poaceae) using various numerical techniques. II. Can J Bot 56:27

    Article  Google Scholar 

  • Baum BR (1978b) Genetic relationships in Triticeae based on computations of Jardine & Sibson Bk clusters. Can J Bot 56:2948

    Google Scholar 

  • Baum BR (1983) A phylogenetic analysis of the tribe Triticeae (Poaceae) based on morphological characters of the genera. Can J Bot 61:518–535

    Google Scholar 

  • Blanco A, Simeone R, Tanzarella OA (1983) Morphology and chromosome pairing of a hybrid between Triticum durum Desf. and Haynaldia villosa (L.) Schur. Theor Appl Genet 64:333–337

    Article  Google Scholar 

  • Blanco A, Simeone R, Resta P (1987a) The addition of Dasypyrum villosum (L.) Candargy chromosomes to durum wheat (Triticum durum Desf.). Theor Appl Genet 74:328–333

    Article  Google Scholar 

  • Blanco A, Lafiandra D, Resta P (1987b) Electrophoretic patterns of durum wheat–Dasypyrum villosum (L.) Candargy lines. Genet Agr 41(3):286

    Google Scholar 

  • Blanco A, Perrone V, Simeone R (1988) Chromosome pairing variation in Triticum turgidum L.  × Dasypy rum villous (L.) Candargy hybrids and genome affinities. In: Miller TE, Koebner RMD (eds) Proc of the 7th intern wheat genet symp, Cambridge, England, July 13–19, 1:63–67

  • Blanco A, Simeone R, Resta P, De Pace C, Delre V, Caccia R, Scarascia Mugnozza GT, Frediani M, Cremonini R, Cionini PG (1996) Genomic relationships between Dasypyrum villosum (L.) Candargy and D. hordeaceum (Cosson et Durieu) Candargy. Genome 39:83–92

    CAS  PubMed  Google Scholar 

  • Bothmer von R, Claesson L (1990) Production and meiotic pairing of intergeneric hybrids of Triticum × Dasypyrum species. Euphytica 51:109–117

    Google Scholar 

  • Bothmer von R, Claesson L (1998) The hybrid Triticum turgidum ssp. dicoccum × Dasypyrum villosum and the cross and backcrosses to breadwheat, T. aestivum. Hereditas 128:47–52

    Article  Google Scholar 

  • Chen P, Liu D (1986) Identification of Haynaldia villosa chromosomes in wheat alien addition lines. In: Zhensheng L, Swaminathan MS (eds) Proc of the 1st intern symp on chromosome engineering in plants, Xian, China, October 20–25, pp. 31–32

  • Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995a) Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91:1125–1128

    Article  Google Scholar 

  • Chen Q, Conner RL, Laroche A (1995b) Identification of the parental chromosomes of the wheat – alien amphidiploid Agrotana by genomic in situ hybridization. Genome 38:1163–1169

    CAS  Google Scholar 

  • Chen Q, Conner RL, Laroche A (1996) Molecular characterization of Haynaldia villosa chromatin in wheat lines carrying resistance to wheat curl mite colonization. Theor Appl Genet 93:679–684

    Article  CAS  Google Scholar 

  • Chen QZ, Qi ZJ, Feng YG, Wang SL, Chen PD (2002) Structural changes of 4 V chromosome of Haynaldia villosa induced by gametocidal chromosome 3C of Aegilops triuncialis. Acta Genet Sin 29(4):355–358

    PubMed  Google Scholar 

  • Chen X, Shi AN, Shang LM, Leath S, Murphy JP (1997) The resistance of H. villosa to powdery mildew isolates and its expression in wheat background. Acta Phytopathologica Sinica 27(1):17–22

    Google Scholar 

  • De Pace C, Qualset CO (1995) Mating system and genetic differentiation in Dasypyrum villosum (Poaceae) in Italy. Plant Syst Evol 197:123–147

    Article  Google Scholar 

  • Deng GB, Chen J, Ma XR, Pan ZF, Yu MQ, Li XF (2004) Morphology, cytogenetics of intergeneric hybrid between Aegilops tauschii and Dasypyrum villosum. Hereditas 26(2):189–194

    PubMed  Google Scholar 

  • Frederiksen S, von Bothmer R (1989) Intergeneric hybridization between Taeniatherum and different genera of Triticeae, Poaceae. Nord J Bot 9(3):229–240

    Google Scholar 

  • Frederiksen S (1991) Taxonomic studies in Dasypyrum (Poaceae). Nord J Bot 11(2):135–142

    Google Scholar 

  • Frederiksen S, von Bothmer R (1995) Intergeneric hybridizations with Eremopyrum (Poaceae). Nord J Bot 15(1):39–47

    Google Scholar 

  • Friebe B, Cermeño MC, Zeller FJ (1987) C-banding polymorphism and the analysis of nucleolar activity in Dasypyrum villosum (L.) Candargy, its added chromosomes to hexaploid wheat and the amphiploid Triticum dicoccum-D. villosum. Theor Appl Genet 73:337–342

    Article  Google Scholar 

  • Fu TH, Ren ZL, Zhang HQ (1997) Cytogenetic analysis of trigeneric hybrid of Triticum, Dasypyrum and Secale by C-banding technique. J Genet Breed 51(4):335–340

    Google Scholar 

  • Galasso I, Blanco A, Katsiotis A, Pignone D, Heslop-Harrison JS (1997) Genome organization and phylogenetic relationships in the genus Dasypyrum analyzed by Southern and in situ hybridization of total genomic and cloned DNA probes. Chromosoma 106:53–61

    Article  PubMed  CAS  Google Scholar 

  • Gruszecka D (1997) Otrzymanie i charakterystyka mieszańców Secale cereale L. z Dasypyrum villosum (Haynaldia villosa L.). Hodowla Roślin. I Krajowa Konferencja, Poznań 19–20 listopada, pp 135–139

  • Gruszecka D, Makarska E, Praczyk M, Miąc A (2001) Wpływ komponentów rodzicielskich na zawartość składników mineralnych translokacyjnej formy żyta z wykorzystaniem Dasypyrum villosum (Krym, USSR). Biul Magnezol 6(3):260–267

    Google Scholar 

  • Gruszecka D, Miąc A (2002) Wykorzystanie metody RAPD w określaniu mieszańcowego charakteru rodów Secale cereale L. × Dasypyrum villosum (L.) P. Candargy. Zesz Problem Post Nauk Roln 488/I:161–168

    Google Scholar 

  • Halloran GM (1966a) Hybridization of Haynaldia villosa with Triticum aestivum. Austr J Bot 14:355–359

    Article  Google Scholar 

  • Halloran GM (1966b) Pairing between Triticum aestivum and Haynaldia villosa chromosomes. J Heredity 57:233–235

    Google Scholar 

  • Hsiao C, Chatterton NJ, Asay KH, Jensen KB (1995) Phylogenetic relationships of the monogenomic species of the tribe Triticeae (Poaceae), inferred from nuclear rDNA (internal transcribed spacer) sequences. Genome 38(2):211–223

    PubMed  CAS  Google Scholar 

  • Humphries CJ (1978) Dasypyrum (Cosson &. Dur.) T. Durand. In: Heywood VH (ed) Flora Europaea. Notulae systematicae ad floram europaeam spectantes. No 20. Bot J Linn Soc 76:297–384

  • Hyde BB (1953) Addition of individual Haynaldia villosa chromosomes to hexaploid wheat. Am J Bot 40:174–182

    Article  Google Scholar 

  • Jahier J, Tanguy AM, Lucas H (1988) Pairing between Dasypyrum villosum (L.) Candargy and Secale cereale L. chromosomes. In: Miller TE, Koebner RMD (eds) Proc of the 7th intern wheat genet symp, Cambridge, England, July 13–19, 1: 315–321

  • Jan CC, De Pace C, Mc Guire PE, Qualset CO (1986) Hybrids and amphiploids of Triticum aestivum L. and T. turgidum L. with Dasypyrum villosum (L.) Candargy. Z Pflanzenzücht 96:97–106

    Google Scholar 

  • Jiang HR, Dai DQ, Sun DF, Xiao SH (1992) New artificial genetic resources of wheat––several polyploids of TriticumDasypyrum. Scientia Agricultura Sinica 25(1):89

    Google Scholar 

  • Jiang JM, Chen PD, Liu DJ (1989) Morphology and cytogenetics in trigeneric hybrid of Triticum durum × Haynaldia villosa × Secale cereale and its derivatives. J Nanjing Agric University 12(2):1–5

    Google Scholar 

  • Kellogg EA (1989) Comments on genomic genera in the Triticeae (Poaceae) Am J Bot 76:796–805

    Article  Google Scholar 

  • Kellogg EA (1992) Tools for studying the chloroplast genome in the Triticeae (Gramineae): An EcoRI map, a diagnostic deletion, and support for Bromus as an outgroup. Am J Bot 7(2):186–197

    Article  Google Scholar 

  • Kihara H (1937) Genomenanalyse bei Triticum und Aegilops VII. Kurze Ubersicht über die Ergebuisse der Jahre 1934–36. Mem Coll Agric, Kyoto Univ, 41:1–61

    Google Scholar 

  • Knobloch IW (1968) A check list of crosses in Gramineae. University of Michigan, pp 1–170

  • Li H, Chen X, Xin ZH, Ma YZ, Xu HJ (1999) Development and identification of wheat-Haynaldia villosa 6DL/6VS translocation lines with powdery mildew resistance. Scientia Agricultura Sinica 32(5):9–15

    CAS  Google Scholar 

  • Li H, Chen X, Xin ZY, Ma YZ, Xu HJ, Chen XY, Jia X (2005) Development and identification of wheat-Haynaldia villosa T6DL.6VS chromosome translocation lines conferring resistance to powdery mildew. Plant Breed 124:203–205

    Article  CAS  Google Scholar 

  • Linde-Laursen I, Frederiksen S (1991) Comparison of the Giemsa C-banded karyotpes of Dasypyrum villosum (2×) and D. breviaristatum (4×) from Greece Hereditas Landskrona 114(3):237–244

    Google Scholar 

  • Linde-Laursen I, von Bothmer R, Jacobsen N (1992) Relationships in the genus Hordeum: Giemsa C-banded karyotypes. Hereditas 116:111–116

    Google Scholar 

  • Liu DJ, Chen PD, Pei GZ, Wang YN, Qiu BX, Wang SL (1988) Transfer of Haynaldia villosa chromosomes into Triticum aestivum. In: Miller TE, Koebner RMD (eds) Proc of the 7th intern wheat genet symp, Cambridge, England, July 13–19, 1:355–361

  • Liu DJ, Chen PD, Raupp WJ (1995) Determination of homoeologous groups of Haynaldia villosa chromosomes. In: Proc of the 8th intern wheat genetic symp, Chinese Agricultural Scientech Press, Bejing, China, pp 181–185

  • Löve A (1984) Conspectus of the Triticeae. Feddes Repert 95:425–521

    Google Scholar 

  • Lucas H (1986) Contribution à l etude des relations phylogénétique et moléculaire. Thèse de l Université de Rennes I, p. 160

  • Lucas H, Jahier J (1988) Phylogenetic relationships in some diploid species of Triticineae: cytogenetic analysis of interspecific hybrids. Theor Appl Genet 75:498–502

    Article  Google Scholar 

  • Łapiński M, Gruszecka D (1997) Charakterystyka pŁodnego mieszańca Secale cereale × Haynaldia villosa. Krajowa Konferencja „Mieszańce oddalone roślin zbożowych”, Poznań 24 kwietnia, pp 4–5

  • Ma JX, Zhou RH, Jia JZ (1997) Identification of wheat-Haynaldia villosa substitution lines conferring resistance to powdery mildew using genomic in situ hybridization (GISH) and RFLP markers. Acta Genet Sin 24:447–452

    CAS  Google Scholar 

  • Makarska E, Gruszecka D, Miąc A (2004) Zmiany w składzie mineralnym ziarniaków nowych translokacyjnych rodów żyta odmiany Amilo z Dasypyrum villosum L. Candargy. J Elementol 9(3):393–398

    Google Scholar 

  • Mason-Gamer RJ (2004) Reticulate evolution, introgression, and intertribal gene capture in an allohexaploid grass. Syst Biol 53(1):25–37

    Article  PubMed  Google Scholar 

  • Meletti P, Onnis A, Stefani A (1977) Haynaldoticum sardoum Meletti et Onnis e la sua origine. Giorn Bot Ital 111:376

    Google Scholar 

  • Miąc A (2003) Wykorzystanie markerów RAPD do oceny translokacyjnych rodów Secale cereale L. × Dasypyrum villosum L. Candargy. Materiały z I Konferencji Naukowej Doktorantów pt. „Problemy technologii produkcji roślinnej, zwierzęcej i żywności”, Lublin 12–13 czerwca, pp 25–26

  • Minelli S, Ceccarelli M, Mariani M, De Pace C, Cionini PG (2005) Cytogenetics of Triticum × Dasypyrum hybrids and derived lines. Cytogenet Genome Res 109:385–392

    Article  PubMed  CAS  Google Scholar 

  • Montebove L, De Pace C, Jan CC, Scarascia-Mugnozza GT, Qualset CO (1987) Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theor Appl Genet 73:836–845

    Article  CAS  Google Scholar 

  • Nakajima G (1950) Cytological studies of an F1 plant of Haynaldia X rye. Jap J Genet 93:679–684

    Google Scholar 

  • Nakajima G (1951) Cytogenetical studies on intergeneric hybrids between Haynaldia villosa and Secale cereale. 1. Characteristics of an F1 plant of Haynaldia villosa (n = 7) × Secale cereale (n = 7) and maturation divisions in PMC’s. La Kromosoma 9/10:364–369

    Google Scholar 

  • Nakajima G (1960) Karyogenetical studies on the intergeneric F1 hybrids raised between Triticum turgidum and Haynaldia hordeacea. Cytologia 252:208–213

    Google Scholar 

  • Ohta S, Morishita M (2001) Genome relationships in the genus Dasypyrum (Gramineae). Hereditas 135:101–110

    Article  PubMed  CAS  Google Scholar 

  • Ohta S, Koto M, Osada T, Matsuyama A, Furuta Y (2002) Rediscovery of a diploid cytotype of Dasypyrum breviaristatum in Morocco. Genetic Resources and Crop Evolution 49(3):305–312

    Article  Google Scholar 

  • Pignone P, Galasso I, Blanco A, Cremonini R (2000) Heterochromatin organization in metaphase chromosomes and interphase nuclei of Dasypyrum breviaristatum (Lindb) Frederiksen. Acta Soc Bot Polon 69(1):5–9

    Google Scholar 

  • Qi LL, Chen PD, Liu DJ, Zhou B, Zhang SZ (1995) Development of translocation lines of Triticum aestivum with powdery mildew resistance introduced from Haynaldia villosa. In: Proc of the 8th intern wheat genet symp, Chinese Agricultural Scientech Press, Bejing, China, pp. 333–337

  • Qi LL, Wang SL, Chen PD, Liu DJ, Gill BS (1998) Identification and physical mapping of three Haynaldia villosa chromosome––6 V deletion lines. Theor Appl Genet 97:1042–1046

    Article  CAS  Google Scholar 

  • Qi LL, Chen PD, Liu DJ, Gill BS (1999) Homoeologous relationships of Haynaldia villosa chromosomes with those of Triticum aestivum as revealed by RFLP analysis. Genes Genet Syst 74:77–82

    Article  CAS  Google Scholar 

  • Sakamoto S (1973) Patterns of phylogenetic differentiation in the tribe Triticeae. Rep Kihara Inst Biol 24:11–31

    Google Scholar 

  • Sakamoto S (1986) Genome analysis of polyploid form of Haynaldia hordeacea in tribe Triticeae, Gramineae. In: Zhensheng L, Swaminathan MS (eds) Proc 1st intern symp chromosome engineering in plants, pp 52–53

  • Sando WJ (1935) Intergeneric hybrids of Triticum and Secale with Haynaldia villosa. J Agric Res 51:579–800

    Google Scholar 

  • Sarkar P (1957) A new diploid form of Haynaldia hordeacea Hack. Wheat Inf Serv 6:22

    Google Scholar 

  • Schubert V, Bluthner WD, Metzlaff M, Junghans H, Schlegel R (1990) The presence of four cloned highly repeated DNA sequences from rye in Poaceae species and various chromosomal manipulated wheat lines analyzed by squash dot hybridization. Biochemie und Physiologie der Pflanzen 186(2):125–133

    CAS  Google Scholar 

  • Sears ER (1953) Addition of the genome of H. villosa to T. aestivum. Am J Bot 40:168–174

    Article  Google Scholar 

  • Sears ER (1976) Genetic control of chromosome pairing in wheat. Ann Rev Genet 10:31–51

    Article  PubMed  CAS  Google Scholar 

  • Semenova LV (1987) Dasypyrum villosum (L.) Borb.––a wild relative of wheat species of subgenus Boeoticum Migusch. Et Dorof. Sbornik Nauchnykh Trudov po Prikladno Botanike, Genetike i Selektsii 111:91–92

    Google Scholar 

  • Shang LM, Chen X, Xiao SH, Xu HJ, Shi AN (1997) Genetic and biochemical identification of common wheat-Haynaldia villosa germplasms. Acta Agron Sinica 23(2):159–164

    Google Scholar 

  • Shewry PR, Parmar S, Pappin DJC (1987) Characterization and genetic control of the prolamins of Haynaldia villosa: relationship to cultivated species of the Triticeae (rye, wheat and barley). Biochem Genet 25:309–325

    Article  PubMed  CAS  Google Scholar 

  • Shu G, Muthukrishnan S, Liang GH, Paulsen GM (1993) Restriction fragment patterns of chloroplast and mitochondrial DNA of Dasypyrum villosum (L.) Candargy and wheats. Theor Appl Genet 87:44–48

    Article  CAS  Google Scholar 

  • Stefani A, Meletti P, Onnis A (1983) New data on the experimental intergeneric hybrid Triticum durum Desf. × Haynaldia villosa Schur. Z Pflanzenzücht 90:236–246

    Google Scholar 

  • Stefani A, Meletti P, Onnis A (1987) Morphological characteristics of the experimental allopoliploid Triticum durum × Haynaldia villosa (2n = 42). Can J Bot 65:1948–1951

    Google Scholar 

  • Sun GL, Yen C, Yang JL, Wu BH (1995) Production and cytogenetics of intergeneric hybrids between Triticum durum-Dasypyrum villosum amphidiploid and Psathyrostachys huashanica. Euphytica 81(1):7–11

    Article  Google Scholar 

  • Tyankova ND (1999) Intergeneric and trigeneric wheat hybrids––production and cytological characteristics. Cer Res Comm 27(1–2):71–77

    Google Scholar 

  • Uslu E, Reader SM, Miller TE (1999) Characterization of Dasypyrum villosum (L.) Candargy chromosomes by fluorescent in situ hybridization. Hereditas 131:129–134

    Article  Google Scholar 

  • Vershinin AV, Heslop-Harrison JS (1998) Comparative analysis of the nucleosomal structure of rye, wheat and their relatives. Plant Mol Biol 36:149–161

    Article  PubMed  CAS  Google Scholar 

  • Vershinin AV, Alkhimova EG, Heslop-Harrison JS (1996) Molecular diversification of tandemly organized DNA sequences and heterochromatic chromosome regions in some Triticeae species. Chromosome Res 4:517–525

    Article  PubMed  CAS  Google Scholar 

  • West JG, McIntyre CL, Apples R (1988) Evolution and systematic relationships in the Triticeae (Poaceae). Plant Syst Evol 160:1–28

    Article  Google Scholar 

  • Xia G, Li Z, Wang S, Xiang F, Liu J, Chen P, Liu D (1998) Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa. Plant Sci 137:217–223

    Article  CAS  Google Scholar 

  • Yang ZJ, Li GR, Feng J, Jiang HR, Ren ZL (2005) Molecular cytogenic characterization and disease resistance observation of wheat-Dasypyum breviaristatum partial amphiploid and its derivatives. Hereditas 142:80–85

    Article  PubMed  Google Scholar 

  • Yildirim A, Jones SS, Murray TD (1998) Mapping a gene conferring resistance to Pseudocercosporella herpotrichoides on chromosome 4 V of Dasypyrum villosum in a wheat background. Genome 41:1–6

    Article  CAS  Google Scholar 

  • Yu MQ, Chen J, Deng GB, Cerbah M, Ma XR, Panaud O, Yakovlev S (2001) Identification for H. villosa chromatin in wheat lines using genomic in situ hybridization, C-banding and gliadin electrophoresis techniques. Euphytica 121:157–162

    Article  CAS  Google Scholar 

  • Yuan WY, Sun SC, Liu SX, Sun Y, Tomita M, Yasumuro Y (1997) Production, fertility and cytology of tetrageneric hybrids involving Triticum, Agropyron, Haynaldia and Secale. Wheat Inf Serv 84:13–18

    Google Scholar 

  • Yuan WY, Tomita M, Sun SC, Yasumuro Y (1998) Introduction of multi––alien chromatin carrying different powdery mildew – resistant genes from rye and Haynaldia villosa into wheat genome. Genes Genet Syst 73:377–384

    Article  CAS  Google Scholar 

  • Zhou A, Xia G (2005) Introgression of the Haynaldia villosa genome into γ-ray-induced asymmetric somatic hybrids of wheat. Plant Cell Rep 24:289–296

    Article  PubMed  CAS  Google Scholar 

  • Zhou YH, Wu BH, Fu TH, Zheng YL (1999) Morphology, fertility and cytogenetics of integeneric hybrid between Roegneria kamoji Ohwi and Dasypyrum villosum (L.) Candargy (Poaceae: Triticeae). Acta Phytotaxonomica Sinica 37(2):125–130

    Google Scholar 

  • Zhou A, Xia G, Zhang X, Chen H, Hu H (2001) Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestivum and Haynaldia villosa Schur. Mol Genet Genomics 265:387–393

    Article  PubMed  CAS  Google Scholar 

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Grądzielewska, A. The genus Dasypyrum––part 1. The taxonomy and relationships within Dasypyrum and with Triticeae species. Euphytica 152, 429–440 (2006). https://doi.org/10.1007/s10681-006-9232-2

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