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Mapping QTLs for Witches' Broom (Crinipellis Perniciosa) Resistance in Cacao (Theobroma Cacao L.)

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Abstract

Molecular markers (RAPD, AFLP and microsatellites) were used to generate a linkage map and to identify QTLs associated to witches' broom (Crinipellis perniciosa) resistance in cacao (Theobroma cacao), using 82 individuals of an F2 population derived from the clones ICS-1 (susceptible) and Scavina-6 (resistant). Fifteen evaluations of the number of brooms have been carried out in six years (1997–2002). In order to increase the precision and accuracy in the measures of resistance, each F2 plant was cloned in three replications in a randomized block design with single-tree plots and evaluated over 2 years. Three hundred and forty-two markers were obtained, being 33 microsatellites, 77 AFLPs and 232 RAPDs. The distribution of the number of brooms in the F2 population was skewed to resistance, suggesting the involvement of major genes controlling resistance and the repeatability estimated for resistance was 44%. A strong putative QTL was detected as being related to witches' broom resistance. Associated to this QTL, the microsatellite mTcCIR35 explained 35.5% of the phenotypic variation in resistance. This marker is being used for marker-assisted selection in Scavina-6 progenies, including those selected in private plantations, as an auxiliary tool to the phenotypic selection.

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Authors and Affiliations

  1. Centro de Pesquisas do Cacau, CEPLAC, Itabuna, BA, Brazil

    Fábio Gelape Faleiro, Uilson Vanderlei Lopes, José Luis Pires, Milton Macoto Yamada & Cláudia Fortes Ferreira

  2. Currently Centro de Pesquisa Agropecuária dos Cerrados, Embrapa, Planaltina, DF, Brazil

    Fábio Gelape Faleiro

  3. Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, Brazil

    Vagner Tibaldi Queiroz, Cláudia Teixeira Guimarães, Everaldo Gonçalves Barros & Maurílio Alves Moreira

  4. Departamento de Ciências Agrárias, Universidade Estadual Norte Fluminense, Campos, RJ, Brazil

    Ioná Santos Araújo, Messias Gonzaga Pereira & Gonçalo Apolinário de Souza Filho

  5. USDA/ARS, Miami, USA

    Raymond Schnell

Authors
  1. Fábio Gelape Faleiro
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  2. Vagner Tibaldi Queiroz
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  3. Uilson Vanderlei Lopes
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  4. Cláudia Teixeira Guimarães
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  5. José Luis Pires
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  11. Cláudia Fortes Ferreira
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  12. Everaldo Gonçalves Barros
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  13. Maurílio Alves Moreira
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Correspondence to Uilson Vanderlei Lopes.

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Faleiro, F.G., Queiroz, V.T., Lopes, U.V. et al. Mapping QTLs for Witches' Broom (Crinipellis Perniciosa) Resistance in Cacao (Theobroma Cacao L.). Euphytica 149, 227–235 (2006). https://doi.org/10.1007/s10681-005-9070-7

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  • DOI: https://doi.org/10.1007/s10681-005-9070-7

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