Abstract
The columnar phenotype is a very valuable genetic resource for apple breeding because of its compact growth form determined by the dominant gene Co. Using bulked segregant analysis combined with several DNA molecular marker techniques to screen the F1 progeny of Spur Fuji × Telamon (heterozygous for Co), 9 new DNA markers (6 RAPD, 1 AFLP and 2 SSRs) linked to the Co gene were identified. A total of 500 10-mer random primers, 56 pairs of selective AFLP primers and 8 SSR primer pairs were screened. One RAPD marker S1142682, and the AFLP marker, E-ACT/M-CTA346, were converted into SCAR markers designated SCAR682 and SCAR216, respectively. These markers will enable early selection in progenies where Co is difficult to identify. The Co gene was located between the SSR markers CH03d11 and COL on linkage group 10 of the apple genetic linkage map. Finally, a local genetic map of the region around the Co gene was constructed by linkage analysis of the nine new markers and three markers developed earlier.
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Tian, YK., Wang, CH., Zhang, JS. et al. Mapping Co, a gene controlling the columnar phenotype of apple, with molecular markers. Euphytica 145, 181–188 (2005). https://doi.org/10.1007/s10681-005-1163-9
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DOI: https://doi.org/10.1007/s10681-005-1163-9