Abstract
Large-scale marker-assisted selection requires highly reproducible, consistent and simple markers. The use of genetic markers is important in woody plant breeding in general, and in apple in particular, because of the high level of heterozygosity present in Malus species. We present here the transformation of two RAPD markers, which we found previously to be linked to the major scab resistance gene Vf, into more reliable and reproducible markers that can be applied directly to apple breeding. We give an example of how the use of such markers can speed up selection for the introduction of scab resistance genes into the same plant, reducing labour and avoiding time-consuming test crosses. We discuss the nature and relationship of the scab resistance gene Vf to the one present in Nova Easygro, thought to be Vr.
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References
Alston FH (1989) Breeding pome fruit with stable resistance to diseases. In: Gessler C, Butt D, Koller B (eds) Integrated control of pome fruit diseases II. OILB-WPRS Bulletin XII/6, pp 90–99
Cheng FS, Brown SK, Weeden NF, Aldwinckle HS (1995) Molecular markers for scab resistance from ‘Nova Easygro’ apple. Plant Genome III Conference (abstract), San Diego, California. January 1995
Chevalier M, Lespinasse Y, Renaudin S (1991) A microscopic study of the different classes of symptoms coded by the Vf gene in apple for resistance to scab (Venturia inaequalis). Plant Pathol 40:249–256
Crosby JA, Janick J, Pecknold PC, Korban SS, Ries SM, Goffreda J, Voordeckers A (1994) Seven disease-resistant apple selections released for growers testing. Fruit Var Jour 48:40
Crowe AD (1975) ‘Nova Easygro’ apple. Fruit Var Jour 29:76
Dayton DF, Williams EB (1968) Independent genes in Malus for resistance to Venturia inaequalis. Proc Am Soc Hort Sci 93:89–94
Dayton DF, Williams EB (1970) Additional allelic genes in Malus for scab resistance of two reaction types. J Am Soc Hort Sci 95:735–736
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Dunemann F, Kahnau R, Schmidt H (1994) Genetic relationships in Malus evaluated by RAPD ‘fingerprinting’ of cultivars and wild species. Plant Breed 113:150–159
Gessler C (1989) Genetic of the interaction Venturia inaequalis-Malus: the conflict between theory and reality. In: Gessler C, Butt D, Koller B (eds) Integrated control of pome fruit diseases II. OILB-WPRS Bulletin XII/6, pp 168–190
Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from an existing mapping population. Nucleic Acids Res 19:6553–6558
Goffreda JC, Polk DF, Rizio GF, Gussman CD, Gianfagna TJ, Crosby JA, Janick J, Pecknold PC, Korban S, Ries SM (1994) Developing scab-resistant apple cultivars with increased quality and ability to store. Fruit Var Jour 48:9
Kellerhals M (1989) Breeding of pome fruit with stable resistance to diseases. In: Gessler C, Butt D, Koller B (eds) Integrated control of pome fruit diseases II. OILB-WPRS Bulletin XII/6, pp. 116–129
King GJ, Alston FH, Battle I, Chevreau E, Gessler C, Janse J, Lindhout P, Manganaris S, Sansavini S, Schimdt H, Tabutt K (1992) The ‘European Apple Genome Mapping Project’ —developing a strategy for mapping agronomic genes in tree species. Euphytica 56:89–94
Koller B, Lehmann A, McDermott J, Gessler C (1993) Identification of apple cultivars using RAPD markers. Theor Appl Genet 85:901–904
Koller B, Gianfranceschi L, Seglias N, McDermott J, Gessler C (1994) DNA-markers linked to the Malus floribunda 821 scab resistance. Plant Mol Biol 26:597–602
Konieczny A, Ausubel FM (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. The Plant Jour 4:403–410
Lespinasse Y (1989) Breeding of pome fruit with stable resistance to diseases: Genes resistance mechanism, present work and prospects. In: Gessler C, Butt D, Koller B (eds) Integrated control of pome fruit diseases II. OILB-WPRS Bulletin XII/6, pp 100–115
Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436
Merwin IA, Rosenberger DA, Engle C (1994) Evaluation of four scab-resistant apple varieties compared with ‘Empire’ in New York orchards. Fruit Var Jour 48:54–56
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in a specific genomic region by using a segreaating population. Proc Natl Acad Sci USA 88:9828–9832
Mulcahy DL, Cresti M, Sansavini S, Douglas GC, Linskens HF, Bergamini Mulcahy G, Vignani R, Pancaldi M (1993) The use of random amplified polymorphic DNA to fingerprint apple genotypes. Scien Hort 54:89–96
Paran I, Michelmore (1993) Development of reliable PCR-based markers linked to the downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993
Parisi L, Lespinasse Y, Guillaumes J, Krüger J (1993) A new race of Venturia inaequalis virulent to apple with resistance due to the Vf gene. Phytopathology 83:533–537
Parisi L, Lespinasse Y (1996) Pathogenicity of Venturia inaequalis strains of race 6 on apple clones (Malus sp.). Plant disease (in press)
Rousselle GL, Williams EB, Hough LF (1974) Modification of the level of resistance to apple scab from the Vf gene. In: XIX Int Horti Cong, Warszawa 11–18 September 1974, pp 19–26
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package JoinMap. The Plant Jour 3:739–744
Tartarini S (1995) RAPD markers linked to the Vf gene for scab resistance in apple. Theor Appl Genet (in press)
Williams EB, Kuĉ J (1969) Resistance in Malus to Venturia inaequalis. Annu Rev Phytopathol 7:223–246
Wing RA, Zhang HB, Tanksley SD (1994) Map-based cloning in crop plants. Tomato as a model system. I. Genetic and physical mapping of jointless. Mol Gen Genet 242:681–688
Work TM, Bushway RJ, Perkins LB, Schupp JR, Bushway AA (1994) Comparison of sensory, chemical and color attributes of diseaseresistant apple cultivars. Fruit Var Jour 48:14–19
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Communicated by G. E. Hart
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Gianfranceschi, L., Koller, B., Seglias, N. et al. Molecular selection in apple for resistance to scab caused by Venturia inaequalis . Theoret. Appl. Genetics 93, 199–204 (1996). https://doi.org/10.1007/BF00225746
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DOI: https://doi.org/10.1007/BF00225746