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Cell phone electromagnetic field radiations affect rhizogenesis through impairment of biochemical processes

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Abstract

Indiscriminate adoption and use of cell phone technology has tremendously increased the levels of electromagnetic field radiations (EMFr) in the natural environment. It has raised the concerns among the scientists regarding the possible risks of EMFr to living organisms. However, not much has been done to assess the damage caused to plants that are continuously exposed to EMFr present in the environment. The present study investigated the biochemical mechanism of interference of 900 MHz cell phone EMFr with root formation in mung bean (Vigna radiata syn. Phaseolus aureus) hypocotyls, a model system to study rhizogenesis in plants. Cell phone EMFr enhanced the activities of proteases (by 1.52 to 2.33 times), polyphenol oxidases (by 1.5 to 4.3 times), and peroxidases (by 1.5 to 2.0 times) in mung bean hypocotyls over control. Further, EMFr enhanced malondialdehyde (an indicator of lipid peroxidation), hydrogen peroxide, and proline content, indicating a reactive oxygen species-mediated oxidative damage in hypocotyls. It was confirmed by the upregulation in the activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase, and glutathione reductase) suggesting their possible role in providing protection against EMFr-induced oxidative damage. The study concluded that cell phone radiations affect the process of rhizogenesis through biochemical alterations that manifest as oxidative damage resulting in root impairment.

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References

  • Aloni, R., Aloni, E., Langhans, M., & Ullrich, C. I. (2006). Role of cytokinin and auxin in shaping root architecture: Regulating vascular differentiation lateral root initiation root apical dominance and root gravitropism. Annals of Botany, 97, 888–893.

    Article  Google Scholar 

  • Atak, Ç., Emiroğlu, Ö., Alikamanoğlu, S., & Rzakoulieva, A. (2003). Stimulation of regeneration by magnetic field in soybean (Glycine max L Merrill) tissue cultures. Journal of Cellular and Molecular Biology, 2, 113–119.

    Google Scholar 

  • Atak, Ç., Çelik, Ö., Olgun, A., Alikamanoğlu, S., & Rzakoulieva, A. (2007). Effect of magnetic field on peroxidase activities of soybean tissue culture. Biotechnolology and Biotechnological Equipments, 21, 166–171.

    CAS  Google Scholar 

  • Balmori, A. (2009). Electromagnetic pollution from phone masts. Effects on wildlife. Pathophysiology, 16, 191–199.

    Article  Google Scholar 

  • Bates, L. S. , Waldren, R. P. , & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205–207.

    Article  CAS  Google Scholar 

  • Batish, D. R. , Singh, H. P. , Setia, N., Kaur, S., & Kohli, R.K. (2006). Effect of 2-benzoxazolinone (BOA) on seedling growth and associated biochemical changes in mung bean (Phaseolus aureus). Zeitschrift für Naturforschung, Section C, 61, 709–714.

    CAS  Google Scholar 

  • Batish, D. R. , Singh, H. P. , Kaur, S., Kohli, R. K. , & Yadav, S. S. (2008). Caffeic acid affects early growth and morphogenetic response of hypocotyl cuttings of mung bean (Phaseolus aureus). Journal of Plant Physiology, 165, 297–305.

    Article  CAS  Google Scholar 

  • Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276–286.

    Article  CAS  Google Scholar 

  • Belyavskaya, N. A. (2004). Biological effects due to weak magnetic field on plants. Advances in Space Research, 34, 1566–1574.

    Article  CAS  Google Scholar 

  • Blank M., & Goodman, R. (2009). Electromagnetic fields stress living cells. Pathophysiology, 16, 71–78.

    Article  Google Scholar 

  • Cakmak, I., & Marschner, H. (1992). Magnesium deficiency and high light intensity enhance activities of superoxide dismutase ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology, 98, 1222–1227.

    Article  CAS  Google Scholar 

  • Egley, G. H., Paul, R. N., Vaughn, K. C., & Duke, S. O. (1983). Role of peroxidase in the development of water impermeable seed coats in Sida spinosa L. Planta, 157, 224–232.

    Article  CAS  Google Scholar 

  • Foyer, C. H., & Halliwell, B. (1976). Presence of glutathione and glutathione reductase in chloroplasts, a proposed role in ascorbic acid metabolism. Planta, 133, 21–25.

    Article  Google Scholar 

  • Günes, T. (2000). Peroxidase and IAA-oxidase activities during rooting in cuttings of three poplar species. Turkish Journal of Botany, 24, 97–101.

    Google Scholar 

  • Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplasts I kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry & Biophysics, 125, 189–198.

    Article  CAS  Google Scholar 

  • Hyland, G. J. (2000). Physics and biology of mobile telephony. The Lancet, 356, 1833–1936

    Article  CAS  Google Scholar 

  • Johansson, O. (2009). Disturbance of the immune system by electromagnetic fields—A potentially underlying cause for cellular damage and tissue repair reduction which could lead to disease and impairment. Pathophysiology, 16, 157–177.

    Article  Google Scholar 

  • Lowry, O. H., Rosebrough, N. T., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the folin-phenol reagent. Journal of Biological Chemistry, 193, 265–278.

    CAS  Google Scholar 

  • Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant & Cell Physiology, 22, 867–880.

    CAS  Google Scholar 

  • Parola, A., Kost, D., Katsir, G., Monselise, E., & Cohen-Luria, R. (2005). Radical scavengers suppress low frequency EMF enhanced proliferation in cultured cells and stress effects in higher plants. The Environmentalist, 25, 103–111.

    Article  Google Scholar 

  • Passardi, F., Penel, C., & Dunand, C. (2004). Performing the paradoxical: How plant peroxidases modify the cell wall. Trends in Plant Sciences, 9, 534–540.

    Article  CAS  Google Scholar 

  • Sage, C., & Carpenter, D. O. (2009). Public health implications of wireless technologies. Pathophysiology, 16, 233–246.

    Article  Google Scholar 

  • Sandu, D. D., Goiceanu, I. C., Ispas, A., Creanga, I., Miclaus, S., & Creanga, D. E. (2005). A preliminary study on ultra high frequency electromagnetic fields effect on black locust chlorophylls. Acta Biologia Hungarica, 56, 109–117.

    Article  CAS  Google Scholar 

  • Sharma, V. P., Singh, H. P., Kohli, R. K., & Batish, D. R. (2009). Mobile phone radiation inhibits Vigna radiata (mung bean) root growth by inducing oxidative stress. Science of Total Environment, 407, 5543–5547.

    Article  CAS  Google Scholar 

  • Singh, H. P., Kaur, S., Batish, D.R., & Kohli, R.K. (2009). Caffeic acid inhibits in-vitro rooting in mung bean (Vigna radiata (L) Wilczek) hypocotyls by inducing oxidative stress. Plant Growth Regulation, 57, 21–30.

    Article  CAS  Google Scholar 

  • Tambiev, A. K., & Kirikova, N. N. (2000). Effect of EHF radiation on metabolism of cyanobacteria Spirulina platensis and other photosynthesizing organisms. Critical Reviews in Biomedical Engineering, 28, 589–602.

    CAS  Google Scholar 

  • Tkalec, M., Malarić, K., & Pevalek-Kozlina, B. (2005). Influence of 400, 900 and 1900 Mhz electromagnetic fields on Lemna minor growth and peroxidase activity. Bioelectromagnetics, 26, 185–193.

    Article  CAS  Google Scholar 

  • Tkalec, M., Malarić, K., & Pevalek-Kozlina, B. (2007). Exposure to radiofrequency radiation induces oxidative stress in duckweed Lemna minor L. Science of Total Environment, 388, 78–89.

    Article  CAS  Google Scholar 

  • Tkalec, M., Malarić, K., Pavlica, M., Pevalek-Kozlina, B., & Vidaković-Cifrek, Z. (2009). Effects of radiofrequency electromagnetic fields on seed germination and root meristematic cells of Allium cepa L. Mutation Research, 672, 76–81.

    CAS  Google Scholar 

  • Velikova, V., Yordanov, I., & Edreva, A. (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science, 151, 59–66.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Environment and Vocational Studies, Panjab University, Chandigarh, 160014, India

    Harminder Pal Singh & Ved Parkash Sharma

  2. Department of Botany, Panjab University, Chandigarh, 160014, India

    Daizy Rani Batish & Ravinder Kumar Kohli

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  1. Harminder Pal Singh
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  2. Ved Parkash Sharma
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  3. Daizy Rani Batish
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  4. Ravinder Kumar Kohli
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Correspondence to Harminder Pal Singh.

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Singh, H.P., Sharma, V.P., Batish, D.R. et al. Cell phone electromagnetic field radiations affect rhizogenesis through impairment of biochemical processes. Environ Monit Assess 184, 1813–1821 (2012). https://doi.org/10.1007/s10661-011-2080-0

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  • DOI: https://doi.org/10.1007/s10661-011-2080-0

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