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Toxicity of Hg, Cu and Zn on early developmental stages of the European clam (Ruditapes decussatus) with potential application in marine water quality assessment

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Abstract

The toxicity of mercury, zinc and copper on sperm viability, fertilisation and embryogenesis of Ruditapes decussatus was examined. Cu did not affect sperm viability at all the concentrations tested. Conversely, the Zn and Hg significantly (P < 0.01) reduced sperm viability only at the highest concentration (respectively 512 and 256 μg/l). Cu caused a significant decrease (p < 0.05) of less than 6% in the fertilisation rate at 128 μg/l and Zn of up to 13% at 64 μg/l. Hg significantly (p < 0.01) inhibited fertilisation at concentrations as low as 32 μg/l. The median effective concentrations (EC50) reducing rates of embryogenesis by 50% were 21.1 μg Hg/l (0.1 μM), 46.3 μg Cu/l (0.72 μM) and 43.4 μg Zn/l. Therefore, Hg is up to seven times more toxic than Cu (on a molar basis). Ecotoxicity of mercury on larvae survival was also assessed in this work. Result showed a significant (p < 0.05) reduction of survival after exposure to 4 and 12 μg/l of Hg. The fertilisation rate and embryogenesis were the most sensitive endpoints, although the latter is more advisable for routine assessment of seawater quality because of its greater sensibility.

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References

  • Beiras, R., & Albentosa, M. (2004). Inhibition of embryo development of the commercial bivalves Ruditapes decussatus and Mytilus galloprovincialis by trace metals; implications for the implementation of seawater quality criteria. Aquaculture, 230, 205–213.

    Article  Google Scholar 

  • Beiras, R., & His, E. (1994). Effects of dissolved mercury on embryogenesis, survival, growth and metamorphosis of Crassostrea gigas oyster larvae. Marine Ecology Progress Series, 113, 95–103.

    Article  Google Scholar 

  • Beiras, R., His, E., & Seaman, M. N. L. (1998). Effects of storage temperature and duration on toxicity of sediments assessed by Crassostrea gigas oyster embryo bioassay. Environmental Toxicology and Chemistry, 17(10), 2100–2105.

    CAS  Google Scholar 

  • Bellas, J., Vazquez, E., & Beiras, R. (2001). Toxicity of Hg, Cu, Cd, and Cr on early developmental stages of Ciona intestinalis (Chordata, Ascidiacea) with potential application in marine water quality assessment. Water Research, 35(12), 2905–2912.

    Article  CAS  Google Scholar 

  • Bellas, J., Beiras, R., & Vazquez, E. (2003). A standardisation of Ciona intestinalis (Chordata, Ascidiacea) embryo-larval bioassay for ecotoxicological studies. Water Research, 37, 4613–4622

    Article  CAS  Google Scholar 

  • Brereton, A., Lord, H., Thornton, I., & Webb, J. S. (1973). Effect of zinc on growth and development of larvae of the Pacific oyster Crassostrea gigas. Marine Biology, 19, 96–101.

    Article  CAS  Google Scholar 

  • Burovina, I. V., Motavkin, P. A., Pivovarova, N. P., & Skul’skii, I. A. (1982). Effect of copper on fertilisation and ion composition of oocytes of the seaurchin Strongylocentrotus nudus. Sov. Journal of Marine Biology, 7(6), 381–387.

    Google Scholar 

  • Calabrese, A., Collier, R. S., Nelson, D. A., & Maclnnes, J. R. (1973). The toxicity of heavy metals to embryos of the American oyster Crassostrea virginica. Marine Biology, 18, 162–166.

    Article  CAS  Google Scholar 

  • Cima, F., Ballarin, L., Bressa, G., Martinucci, G., & Burighel, P. (1996). Toxicity of organotin compounds on embryos of a marine invertebrate (Styela plicata; Tunicata). Ecotoxicology and Environmental Safety, 35, 174–182.

    Article  CAS  Google Scholar 

  • Dinnel, P. A., Link, J. M., Stober, Q. J., Letourneau, M. W., & Roberts, W. E. (1989). Comparative sensitivity of sea urchin bioassays to metals and pesticides. Archives of Environmental Contamination and Toxicology, 18, 748–755.

    Article  CAS  Google Scholar 

  • Franchet, C., Goudeau, M., & Goudeau, H. (1997). Mercuric ions impair the fertilisation potential, the resumption of meiosis, the formation of male pronucleus, and increase polyspermy, in the egg of the ascidian Phallusia mammillata. Journal of Experimental Zoology, 278, 255–272.

    Article  CAS  Google Scholar 

  • His, E., & Robert, R. (1982). The danger of treatments by the copper sulphate in conchylicole zone: Toxicity toward crassostrea gigas eggs and larvae. Revue des Travaux de l’Institut des Pêches Maritimes, 42(2), 117–125.

    Google Scholar 

  • His, E., Maurer, D., & Robert, R. (1983). Biological method to estimate sea water content of tributyltin acetate. Journal of Molluscan Studies, 12A(Suppl.), 60–68.

    Google Scholar 

  • His, E., Seaman, M. N. L., & Beiras, R. (1997). A simplification of the bivalve embryogenesis larval development bioassay method for water quality assessment. Water Research, 31, 351–355.

    Article  CAS  Google Scholar 

  • His, E., Beiras, R., & Seaman, M. N. L. (1999). The assessment of marine pollution – Bioassays with bivalve embryos and larvae. Advances in Marine Biology, 37, 1–178.

    Article  Google Scholar 

  • Hoare, K., Beaumont, A. R., & Davenport, J. (1995a). Variation among populations in the resistance of Mytilus edulis embryos to copper: Adaptation to pollution? Marine Ecology Progress Series, 120, 155–161.

    Article  CAS  Google Scholar 

  • Hoare, K., Davenport, J., & Beaumont, A. R. (1995b). Effects of exposure and previous exposure to copper on growth of veliger larvae and survivorship of Mytilus edulis juveniles. Marine Ecology Progress Series, 120, 163–168.

    Article  CAS  Google Scholar 

  • Kobayashi, N., & Okamura, H. (2004). Effects of heavy metals on sea urchin embryo development. 1.Tracing the cause by the effects. Chemosphere, 55, 1403–1412.

    Article  CAS  Google Scholar 

  • Long, E. R., Robertson, A., Wolfe, D. A., Hameedi, J., & Sloan, G. M. (1996). Estimates of the spatial extent of sediment toxicity in major US estuaries. Environmental Science and Technology, 30(3), 585–592.

    Google Scholar 

  • Manzo, S. (2003). Sea urchin embryotoxicity test: Proposal for a simplified bioassay. Ecotoxicology and Environmental Safety, 57, 123–128.

    Article  CAS  Google Scholar 

  • Martin, M., Osborn, K. E., Billig, P., & Glickstein, N. (1981). Toxicities of ten metals to Crassostrea gigas and Mytilus edulis embryos and Cancer magister larvae. Marine Pollution Bulletin, 12(9), 305–308.

    Article  CAS  Google Scholar 

  • Medhioub, M. N., Lymayem, Y., Fathallah, S., Abed, M. M., & Medhioub, A. (2006). Cycle of artificial breeding of the clam Ruditapes decussatus (mollusk lamellibrache). Acte of 13th Scientific journey of agricol research results—Hammamet, 14 & 15 Décember.

  • Nacci, D. E., Jackim, E., & Walsh, R. (1986). Comparative evaluation of three marine toxicity tests: Sea urchin embryo growth test, sea urchin sperm cell toxicity test and Microtox. Environmental Toxicology and Chemistry, 5, 521–526.

    Article  CAS  Google Scholar 

  • Newman, M. C. (1995). Quantitative methods in aquatic ecotoxicology (426 pp.). Boca Raton: Lewis.

    Google Scholar 

  • Nice, H. E. (2005). Sperm motility in the Pacific oyster (Crassostrea gigas) is affected by nonylphenol. Marine Pollution Bulletin, 50, 1668–1674.

    Article  CAS  Google Scholar 

  • Quiniou, F., Judas, A., & Le Squer-Andre, E. (1997). Two bioassays to estimate seawater and sediment toxicity in natural harbour estuaries of Brest. Annals of Océanographic Institute Paris, 73(1), 35–48.

    Google Scholar 

  • Ringwood, A. H. (1992). Comparative sensitivity of gametes and early development stages of a sea urchin (Echinometra mathaei) and a bivalve species (Isognomon californicum) during metal exposures. Archives of Environmental Contamination and Toxicology, 22, 288–295.

    Article  CAS  Google Scholar 

  • Robert, R., His, E., & Maurer, D. (1986). Toxicity of a weedkiller, the atrazine-simazine, on Crassostrea gigas larval stages and on two microalgae, Isochrysis aff-galbana and Chaetoceros calcitrans. Haliotis, 15, 319–325.

    Google Scholar 

  • Vashchenko, M. A., Zhadan, P. M., Malakhov, V. V., & Medvedeva, L. A. (1995). Toxic effect of mercury chloride on gametes and embryos in the sea urchin Strongylocentrotus intermedius. Russ. Journal of Marine Biology, 21(5), 300–307.

    Google Scholar 

  • Vlasova, G. A., & Khristoforova, N. K. (1982). The effect of cadmium on early ontogenesis of the sea urchin Strongylocentrotus intermedius. Sov. Journal of Marine Biology, 8(4), 210–215.

    Google Scholar 

  • Warnau, M., Iacarino, M., de Biase, A., Temara, A., Jangoux, M., Dubois, P., et al. (1996). Spermiotoxicity and embryotoxicity of heavy metals in the echinoid Paracentrotus lividus. Environmental Toxicology and Chemistry, 15, 1931–1936.

    CAS  Google Scholar 

  • Woelke, C. E. (1972). Development of a receiving water quality bioassay criterion based on the 48-hour Pacific oyster, Crassostrea gigas embryo. Washington Department of Fish Technology Rep, 9, 1–93.

    Google Scholar 

  • Zaroogian, G. E., Pesh, G., & Morrison, G. (1969). Formulation of an artificial sea-water medium suitable for oyster larvae development. American Zoologist, 9, 1144.

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire d’Aquaculture, Institut National des Sciences et Technologies de la Mer BP59, route de Khniss, 5000, Monastir, Tunisia

    Salem Fathallah, Mohamed Néjib Medhioub, Amel Medhioub & Mohamed Mejdeddine Kraiem

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  1. Salem Fathallah
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  2. Mohamed Néjib Medhioub
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  3. Amel Medhioub
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  4. Mohamed Mejdeddine Kraiem
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Correspondence to Salem Fathallah.

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Fathallah, S., Medhioub, M.N., Medhioub, A. et al. Toxicity of Hg, Cu and Zn on early developmental stages of the European clam (Ruditapes decussatus) with potential application in marine water quality assessment. Environ Monit Assess 171, 661–669 (2010). https://doi.org/10.1007/s10661-010-1311-0

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