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Alteration in hematology of Labeo rohita under stress of pollution from Lakes of Bangalore, Karnataka, India

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Abstract

Blood is an indicator of physiological condition of an animal. Therefore, a field study was conducted to investigate the hematological parameters of wild population of rohu, Labeo rohita (Ham). The following aspects were evaluated in blood: hemoglobin content, red blood cell (RBC) and white blood cell (WBC) count, packed cell volume (PCV), and mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) values, and in plasma: cholesterol, protein, and glucose levels. For this purpose, rohu fish of varying sizes and weights were sampled from Hebbal (receiving a storm water drain) and Chowkalli lake (received domestic sewage and industrial effluents from various sources and was more polluted than Hebbal lake). It revealed noticeable differences in hemoglobin content, RBC and WBC count, and PCV and MCHC values. Severe anemia can be marked by a significant decrease in RBC count (p < 0.5), hemoglobin content, and PCV and MCHC values, whereas an increase in leukocyte count and MCV values were observed in fish from Chowkalli lake. Fish from lake B had fewer RBC and low concentration of serum protein and cholesterol. Serum concentration of glucose showed initial higher levels and then low concentration (900–1,500 g) in fish from lake B when compared to lake A. The variation in values of different parameters can be attributed to exposure of fish to various types of pollutants present mainly in the Chowkalli lake which receives heavy metals, synthetic detergents, petroleum products, and other acid and alkali substances from the nearby local industries. Other observations of these fish include dark body color and aggressive nature of fish.

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References

  • Acharya, S., Dutta, T., & Das, M. K. (2005). Effect of sublethal levels of nitrite on some blood parameters of juvenile L. rohita (Ham.). Indian Journal of Experimental Biology, 43, 450–454.

    CAS  Google Scholar 

  • APHA, AWWA, WPCF (1995). Standard methods for water-and-wastewater (19th ed.). Washington, DC: American Public Health Association.

    Google Scholar 

  • Awasthi, S. K., Mishra, L. C., & Agnihotri, M. M. (2003). Use of haematopoietic cell renewal systems for assessment of toxic effects of chemicals. Journal of Ecotoxicology and Environmental Monitoring, 13(2), 129–144.

    CAS  Google Scholar 

  • Banerjee, V., & Banerjee, M. (1988). Effect of heavy metal poisoning on peripheral hemogram in H. fossilis (Bloch) mercury, chromium and zinc chlorides (LC50). Comparative Physiology and Ecology, 13, 128–134.

    CAS  Google Scholar 

  • Bansal, S. K., Verma, S. R., Gupta, A. K., & Dalela, R. C. (1980). Predicting long-term toxicity by sub-acute screening of pesticides with larval and early juveniles of four species of freshwater major carp. Ecotoxicology and Environmental Safety, 4, 224–231.

    Article  CAS  Google Scholar 

  • Bhatia, N. P., Sandhu, G. S., & Johal, M. S. (2002). Endosulfan induced changes in blood chemistry of Heteropneustes fossilis. Pollution Research, 21(3), 323–327.

    Google Scholar 

  • Buckley, J. A., Whitmore, C. M., & Matsuda, R. I. (1976). Changes in blood chemistry and blood cell morphology in coho salmon, Oncorhynchus kisutch following exposure to sublethal levels of total residual chlorine in municipal wastewater. Journal of Fish Research Board Canada, 33, 776–782.

    CAS  Google Scholar 

  • Cazenave, J., Wunderlin, D. A., Hued, A. C., & de los Angeles Bistoni, M. (2005). Haematological parameters in a neotropical fish, Corydoras paleatus (Jenyns, 1842) (Pisces, Callichthyidae), captured from pristine and polluted water. Hydrobiologia, 537, 25–33.

    Article  Google Scholar 

  • Cech, Jr., J. J., Bartholow, S. D., Young, P. S., & Hopkins T. E. (1996). Striped bass exercise and handling stress in freshwater: Physiological responses to recovery environment. Transactions of the American Fisheries Society, 125(2), 308–320.

    Article  CAS  Google Scholar 

  • Chandra, S., Ram, R. N., & Singh, I. J. (2001). Toxic effects of carbofuran on certain haematological parameters in yearlings of Cyprinus carpio. Aquaculture, 2, 137–140.

    Google Scholar 

  • Chellan, B., Ramesh, M., & Ramanujam, R. M. (1999). Lethal and sublethal effects of a synthetic detergents on glucose-6-phosphate-dehydrogenase (G-6-P.D.H) enzyme activity in blood of a freshwater teleost, Labeo rohita (Ham, 1822). Indian Journal of Fisheries, 46(4), 397–400.

    Google Scholar 

  • Christensen, G. M., Fiandt, J. T., & Poeschl, B. A. (1978). Cells, protein, and certain physical–chemical properties of brook trout (Salvelinus fontinalis) blood. Journal of Fish Biology, 12, 51–60.

    Article  CAS  Google Scholar 

  • Dacie, J. A., & Lewis, S. M. (1991). Practical haematology A (7th ed.). London: Churchill Livingstone.

    Google Scholar 

  • Das, M., Mukhopadhyay, S., Addiya, S., Chakrabarthi, S., & Chatterjee, G. (1987). Effect of in vitro cadmium administration to rats on certain functional parameters of isolated erythrocytes. Indian Journal of Experimental Biology, 25, 244–248.

    CAS  Google Scholar 

  • Hardikar, B. P., & Gokhale, K. S. (2000). Study of haematological parameters of sewage fed fish, Sarotherodon mossambicus (Peters). Bulletin of Pure and Applied Sciences, 19, 7–13.

    Google Scholar 

  • Hattingh, J. (1976). Blood sugar as an indicator of stress in the freshwater fish, Labeo capensis (Smith). Journal of Fish Biology, 10(2), 191–195.

    Article  Google Scholar 

  • Hochachka, P. W. & Somero, G. N. (1973). Strategies of biochemical adaptation (pp. 317–336). Philadelphia: Saunders.

    Google Scholar 

  • Hymavathi, V., & Rao, L. M. (2000). Effect of sublethal concentration of lead on the haematology and the biochemical constituence of Channa punctata. Bulletin of Pure and Applied Sciences, 19, 1–5.

    Google Scholar 

  • Jana, S., & Bandyopadhyaya, N. (1987). Effect of heavy metals on some biochemical parameters in the freshwater fish, Channa punctatus. Environmental Ecology, 5(3), 488–493.

    Google Scholar 

  • Jiménez, B. D., & Stegeman, J. J. (1990). Detoxification enzymes as indicators of stress. Transactions of the American Fisheries Society, 8, 67–79.

    Google Scholar 

  • Joshi, P. K., Bose, M., & Harish, D. (2002). Haematological changes in the blood of Clarias batrachus exposed to mercuric chloride. Journal of Ecotoxicology and Environmental Monitoring, 12, 119–122.

    CAS  Google Scholar 

  • Karuppasamy, R. (2000). Impact of phenyl mercuric acetate (PMA) on the biomodal respiration in an air-breathing fish C. punctatus (Bloch). Journal of Environmental Pollution, 7, 287–293.

    Google Scholar 

  • Karuppasamy, R., Subathra, S., & Puvaneswari, S. (2005). Haematological responses to exposure to sublethal concentration of cadmium in air-breathing fish C. punctatus (Bloch). Journal of Environmental Biology, 26(1), 123–128.

    CAS  Google Scholar 

  • Larsson, A., Lehtinen, K. J., & Haux, C. (1980). Biochemical and haematological effects of titanium dioxide industrial effluent on fish. Bulletin of Environmental Contamination and Toxicology, 25, 427–435.

    Article  CAS  Google Scholar 

  • Lethtinum, K. J., Kierkegard, A., Jakobson, E., & Wandell, A. (1990). Physiological effects in fish exposed to effluents from mills with six different bleaching processes. Ecotoxicology and Environmental Safety, 19, 33–46.

    Article  Google Scholar 

  • Lowry, O. H., Rosenbrough, N. J., Ferry, A. L., & Randall, R. J. (1951). Protein measurement with folin-phenol reagent. Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  • Manoharan, T., & Subbaiah, G. N. (1982). Toxic and sub-lethal effects of endosulfan on Barbus stigma. Proceedings of the Indian National Academy of Sciences (Animal Sciences), 91, 523–532.

    Article  CAS  Google Scholar 

  • Maruthanayagam, C., & Sharmila, G. (2004). Haematobiochemical variations induced by the pesticide, monocrotophos in Cyprinus carpio during the exposure and recovery periods. Nature Environment and Pollution Technology, 3(4), 491–494.

    CAS  Google Scholar 

  • McCarthy, D. H., Stevenson, J. P., & Roberts, M. S. (1973). Some blood parameters of rainbow trout (Salmo gairdneri Richardson). 1. The Kamloops variety. Journal of Fish Biology, 5, 1–8.

    Article  Google Scholar 

  • McLeavy, D. J., & Brown, D. A. (1974). Growth stimulation and biochemical changes in juvenile Coho salmon (Oncorhynchus kisutch) exposed to bleached kraft pulp mill effluent for 200 days. Journal of the Fisheries Research Board of Canada, 31, 1043–1049.

    Google Scholar 

  • Mulcahy, M. F. (1975). Fish blood changes associated with diseases. A haematological study of pike lymphoma and salmon ulcerative dermal necrosis. In The pathology of fish (pp. 925–944). University of Wisconsin.

  • Nanda, P. (1997). Haematological changes in the common Indian catfish Heteropneustes fossilis under nickel stress. Journal of Ecobiology, 9, 243–246.

    Google Scholar 

  • Ramakritinan, C. M., Kumaraguru, A. K., & Balasubramanian, M. P. (2005). Impact of distillery effluent on Carbohydrate metabolism of freshwater fish, Cyprinus carpio. Ecotoxicology, 14, 693–707.

    Article  CAS  Google Scholar 

  • Sampath, K., James, R., & Akbar Ali, K. M. (1998). Effects of copper and zinc on blood parameters and prediction of their recovery in Oreochromis mossambicus (pisces). Indian Journal of Fisheries, 45(2), 129–139.

    Google Scholar 

  • Samuel, R. M. (1986). Haematology. In: Notes on clinical lab techniques (4th ed.). Madras: Tailor.

    Google Scholar 

  • Saravanan, J. S., & Harikrishnan, R. (1999). Effect of sublethal concentration of copper and endosulphan on haematological parameters of the freshwater fish, Sarotherodon mossambicus (Trewaves). Journal of Ecobiology, 11, 13–18.

    CAS  Google Scholar 

  • Seifter, S., Novie, B., & Muntwyer, E. (1950). The estimation of glycogen with the Anthrone reagent. Archives of Biochemistry, 25, 191–200.

    CAS  Google Scholar 

  • Soivo, A., & Nikinmaa, M. (1981). The swelling of erythrocytes in relation to the oxygen affinity of the blood of the rainbow trout, Salmo gairdneri, Richardson. In Pickering (Ed.), Stress and fish (pp. 49–75).

  • Van Vuren, J. H. J. (1986). The effects of toxicants on the haematology of Labeo umbratus (Teleosti; cyprinidae). Comparative Biochemistry and Physiology, 83C, 155–159.

    Google Scholar 

  • Vosylienë, M. Z. (1999). The effect of heavy metals on haematological indices of fish. Acta Zoologica Lituanica, 9(2), 76–82.

    Google Scholar 

  • Wedemeyer, G. A., & Yasutake, W. T. (1977). Clinical methods for the assessment of environmental stress on fish health. U.S. Fish and Wild Service Technical Paper, 89, 183–191.

    Google Scholar 

  • Wepener, V., Vanvuren, J. H., & Dupreez, H. H. (1992). The effect of hexavalent chromium at different pH values on the haematology of Tilapia sparmani (Cichliodae). Comparative Biochemistry and Physiology, 101C(2), 375–381.

    CAS  Google Scholar 

  • Zlatkis, A., Zak, B., & Boyle, A. J. (1953). A new method for direct determination of serum cholesterol. Journal of Laboratory and Clinical Medicine, 41, 481–492.

    Google Scholar 

Download references

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  1. Aquatic Biology and Fish Toxicology Section, Department of Zoology, Bangalore University, Jnanabharathi, Bangalore, 560056, India

    Bela Zutshi, S. G. Raghu Prasad & R. Nagaraja

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  1. Bela Zutshi
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  2. S. G. Raghu Prasad
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  3. R. Nagaraja
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Correspondence to Bela Zutshi.

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Zutshi, B., Prasad, S.G.R. & Nagaraja, R. Alteration in hematology of Labeo rohita under stress of pollution from Lakes of Bangalore, Karnataka, India. Environ Monit Assess 168, 11–19 (2010). https://doi.org/10.1007/s10661-009-1087-2

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