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Antioxidative responses related to H2O2 depletion in Hypnum plumaeforme under the combined stress induced by Pb and Ni

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Abstract

The short-term responses of H2O2-depletion-related parameters in moss Hypnum plumaeforme to the combined stress induced by Pb and Ni were investigated. The results showed that the Pb and Ni stress induced dose-dependent accumulation of hydrogen peroxide (H2O2). The increase of peroxidase (POD) activity and decrease of ascorbate peroxidase (APX) activity were observed under the combined heavy metal application. The antioxidants, ascorbate (AsA) and proline content, increased significantly when the metals were applied together. The study indicated that the cell damage caused by Pb stress was higher than that caused by Ni stress, Pb and Ni had synergistic effect in inducing the oxidative stress in moss H. plumaeforme, especially under the combination of high concentration of Ni (0.1 and 1.0 mM) and Pb. Content of proline, H2O2 and the activity of POD, all showed a dose-dependent increase under Pb and Ni stress, suggesting their practical value as biomarkers in moss biomonitoring, especially in the case of light pollution caused by heavy metals without the changes in the appearance of mosses.

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Authors and Affiliations

  1. Institute of Mountain Hazards and Environment, Chinese Academy of Science, Chengdu, 610041, China

    Shou-Qin Sun

  2. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, China

    Ming He, Yusufujiang Yusuyin, Wei Han & Jin-Ling Li

  3. College of Life and Environmental Science, Shanghai Normal University, Shanghai, 200234, China

    Tong Cao

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  1. Shou-Qin Sun
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Correspondence to Ming He.

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Sun, SQ., He, M., Cao, T. et al. Antioxidative responses related to H2O2 depletion in Hypnum plumaeforme under the combined stress induced by Pb and Ni. Environ Monit Assess 163, 303–312 (2010). https://doi.org/10.1007/s10661-009-0835-7

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