Summary
Funaria hygrometrica gametophytes were grown in in vitro controlled systems in the presence and absence of lead. Their nuclear genomes were then analyzed directly in situ with A+T and G+C specific fluorochromes, image analysis and statistical data elaboration by specific software, in order to characterize the different fractions of repetitive DNA. The results reveal qualitative and quantitative differences between the nuclear genomes of lead-stressed and unstressed individuals. These differences seem to consist of a significant increase in G+C rich repetitive DNA sequences in nuclei of the stressed individuals. These sequences form well defined agglomerates, generally situated adjacent to the nucleolar region, which increase in both size and number in the presence of lead. Some hypotheses are discussed.
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Abbreviations
- DAPI:
-
4′,6-diamidino-2-phenylindole
- BBM:
-
bold basal medium
References
Basile A, Giordano S, Spagnuolo V, Alfano F, Castaldo-Cobianchi R (1995) Effect of lead on morphogenesis and developmental pattern in the protonema of the mossFunaria hygrometrica. Ann Bot (in press)
Bassi P (1990) Quantitative variations of nuclear DNA during plant development: a critical analysis. Biol Rev 65: 185–225
— (1991) Repetitive/non coding DNA: a possible link between environment and gene expression in plants? Biol Zentralbl 10: 1–13
— (1992) Link between environment and gene expression. Rice Biotech Q 10: 37–38
Bassi P (1993) Is repetitive DNA involved in theNarcissus male gametogenesis? Protoplasma 174: 134–141
—, Ferrucci L (1993) In situ molecular-genetic characterization of repetitive DNA: a different experimental approach. Caryologia 44: 27–33
—, Cremonini R, Cionini PG (1982) Cytological localization of inverted repeated DNA sequences inVicia faba. Chromosoma 85: 453–459
Cullis CA (1981) Environmental induction of heritable changes in flax: defined environments inducing changes in rDNA and peroxidase isozyme band pattern. Heredity 47: 87–94
—, Charlton L (1981) The induction of ribosomal DNA changes in flax. Plant Sci Lett 20: 213–217
—, Clearly W (1986) Rapidly varying DNA sequences in flax. Can J Genet Cytol 28: 252–295
Ferraro M, Lavia P (1985) Differential gene activity visualized on sister chromatids after replication in the presence of 5-azacytidine. Chromosoma 91: 307–312
Gense MT (1980) Heterogeneity of nuclear DNA labelling from intact, wounded and virus-infected tobacco leaves. Cell Diff 9: 125–133
Guillé E, Quetier F (1973) Heterochromatic, redundant and metabolic DNAs: a new hypothesis about their structure and function. Prog Biophys Mol Biol 27: 123–142
Krupa J (1964) Studies on the physiology of germination of spores ofFunaria hygrometrica. Acta Soc Bot Pol 33: 179–192
Maffei M, Mucciarelli M, Scannerini S (1994) Are leaf area index (LAI) and flowering related to oil productivity in peppermint? Flavour Fragr J 9: 119–124
Nagl W (1990) Gene amplification and related events. In: Bajaj YPS (ed) Somaclonal variation in crop improvement. Springer, Berlin Heidelberg New York Tokyo, pp 153–201 (Biotechnology in agriculture and forestry, vol 11)
—, Rücker W (1976) Effects of phytohormones on thermal denaturation profiles ofCymbidium DNA: indication of differential DNA replication. Nucleic Acids Res 3: 2033–2039
Natali L, Cavallini A, Cremonini R, Bassi P, Cionini PG (1986) Amplification of nuclear DNA sequences during induced plant cell dedifferentiation. Cell Diff 18: 157–161
Nichols HW (1973) Growth media in fresh water. In: Stein JR (ed) Handbook of phycological methods. Cambridge University Press, Cambridge, pp 7–24
Sampson M, Clarkson D, Davies DD (1965) DNA synthesis in aluminium-treated roots of barley. Science 148: 1476–1477
Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307–324
Sissoeff I, Grisvard J, Guillé E (1976) Presence of divalent cations bound to reiterative DNA sequences in higher plant and animal tissues. Müller-Bérat et tal (eds) Progress in differentiation research. North-Holland, Amsterdam, pp 197–204
Van Oostveldt P, Van Parijs R (1975) Effect of light on nucleic acid synthesis and polyploidy level in elongating epicotyl cells ofPisum sativum. Planta 124: 287–295
Zuckerkandl E (1981) A general function of noncoding polynucleotide sequences. Mol Biol Rep 7: 149–158
— (1986) Polite DNA: functional density and functional compatibility in genomes. J Mol Evol 24: 12–27
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Bassi, P., Basile, A., Stefanini, A. et al. Effects of lead on the nuclear repetitive DNA of the mossFunaria hygrometrica (Bryophyta). Protoplasma 188, 104–108 (1995). https://doi.org/10.1007/BF01276800
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DOI: https://doi.org/10.1007/BF01276800