Skip to main content
SpringerLink
Log in

A loop mediated isothermal amplification (LAMP) assay for rapid and reliable detection of Anguina wevelli, a grass parasitic nematode

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Anguina wevelli is a pathogenic grass parasitic nematode, however it is difficult to identify based simply on morphology. This study developed a loop-mediated isothermal amplification (LAMP) assay to detect A. wevelli. The LAMP method developed could specifically detect A. wevelli in 45 min, and the detection limit was 1/80000 of the total DNA extracted from a single juvenile (J2), an equivalent of 2.5 pg of DNA. This is the first report of the detection of Anguina spp. by using a LAMP-based method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Chizhov, V. N., & Subbotin, S. A. (1990). Plant-parasitic nematodes of the subfamily Anguininae (Nematoda, Tylenchida). Morphology, trophic specialization, systematics. Zoologicheskiĭ Zhurnal, 69(1), 15–26.

    Google Scholar 

  • Duan, Y. B., Ge, C. Y., Zhang, X. K., Wang, J. X., & Zhou, M. G. (2014). Development and evaluation of a novel and rapid detection assay for Botrytis Cinerea based on loop-mediated isothermal amplification. PloS One, 9(10), e111094.

    Article  PubMed  PubMed Central  Google Scholar 

  • Edgar, J. A., Frahn, J. L., Cockrum, P. A., Anderton, N., Jago, M. V., Culvenor, C. C., Jones, A. J., Murray, K., & Shaw, K. J. (1982). Corynetoxins, causative agents of annual ryegrass toxicity; their identification as tunicamycin group antibiotics. Journal of the Chemical Society Chemical Communication, 4(4), 222–224.

    Article  Google Scholar 

  • Gasser, R. B., & Newton, S. E. (2000). Genomic and genetic research on bursate nematodes: significance, implications and prospects. International Journal for Parasitology, 30(4), 509–534.

    Article  CAS  PubMed  Google Scholar 

  • Griesbach, J., Chitambar, J. J., Hamerlynck, M. J., & Duarte, E. O. (1999). A comparative analysis of extraction methods for the recovery of Anguina sp. from grass seed samples. Journal of Nematology, 33, 635–640.

    Google Scholar 

  • Iwamoto, T., Sonobe, T., & Hayashi, K. (2003). Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare. Journal of Clinical Microbiology, 41(6), 2616–2622.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kikuchi, T., Aikawa, T., Oeda, Y., Karim, N., & Kanzaki, N. (2009). A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology, 99(12), 1365–1369.

    Article  CAS  PubMed  Google Scholar 

  • Krall, E. L. (1991). Wheat and grass nematodes: Anguina, Subanguina, and related genera. In W. R. Nickle (Ed.), Manual of agricultural nematology (pp. 721–760). New York: Marcel Dekkersss.

    Google Scholar 

  • Li, W. B., Yan, Z. H., & Nakhla, M. K. (2015). Real-time PCR for detection and identification of Anguina funesta, A. agrostis, A. tritici, and A. pacificae. Plant Disease, 99(11), 1584–1589.

    Article  CAS  Google Scholar 

  • Lin, B. R., Wang, H. H., Zhuo, K., & Liao, J. L. (2016). Loop-mediated isothermal amplification for the detection of Tylenchulus semipenetrans in soil. Plant Disease, 100(5), 877–883.

    Article  CAS  Google Scholar 

  • Liu, X. T., Wang, H. H., Lin, B. R., Tao, Y., Zhuo, K., & Liao, J. L. (2016). Loop-mediated isothermal amplification based on the mitochondrial COI region to detect Pratylenchus zeae. European Journal of Plant Pathology. doi:10.1007/s10658-016-1102-8.

  • Ma, Y. G., Wang, J. C., Xie, H., Zhou, C. N., Du, Y., Huang, G. M., & Li, F. R. (2006). Multiple-PCR detection on three species of Anguina genus. Acta Phytopathologica Sinica, 6, 508–511.

    Google Scholar 

  • Maeda, H., Kokeguchi, S., Fujimoto, C., Tanimoto, I., Yoshizumi, W., Nishimura, F., & Takashiba, S. (2005). Detection of periodontal pathogen Porphyromonas gingivalis by loopmediated isothermal amplification method. FEMS Immunology and Medical Microbiology, 43(2), 233–239.

    Article  CAS  PubMed  Google Scholar 

  • Mori, Y., Nagamine, K., Tomita, N., & Notomi, T. (2001). Detection of loop mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochemical and Biophysical Research Communications, 289, 150–154.

    Article  CAS  PubMed  Google Scholar 

  • Nagamine, K., Hase, T., & Notomi, T. (2002). Accelerated reaction by loop mediated isothermal amplification using loop primers. Molecular and Cellular Probes, 16, 223–229.

    Article  CAS  PubMed  Google Scholar 

  • Niu, J. H., Jian, H., Guo, Q. X., Chen, C. L., Wang, X. Y., Liu, Q., & Guo, Y. D. (2012). Evaluation of loop-mediated isothermal amplification (LAMP) assays based on 5S rDNA-IGS2 regions for detecting Meloidogyne enterolobii. Plant Pathology, 61(4), 809–819.

    Article  CAS  Google Scholar 

  • Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop mediated isothermal amplification of DNA. Nucleic Acids Research, 28(12), e63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Peng, J., Shi, M., Xia, Z., Huang, J., & Fan, Z. (2012). Detection of cucumber mosaic virus isolates from banana by one-step reverse transcription loop-mediated isothermal amplification. Archives of Virology, 157, 2213–2217.

    Article  CAS  PubMed  Google Scholar 

  • Powers, T. O., Todd, T. C., Burnell, A. M., Murray, P. C. B., Fleming, C. C., Szalanski, A. L., Adams, B. A., & Harris, T. S. (1997). The rDNA internal transcribed spacer region as a taxonomic marker for nematodes. Journal of Nematology, 29(4), 441–450.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Powers, T. O., Szalanski, A. L., Mullin, P. G., Harris, T. S., Bertozzi, T., & Griesbach, J. A. (2001). Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 33(4), 191–194.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Price, P. C., Fisher, J. M., & Kerr, A. (1979). One Anguina funesta n. sp. and its association with Corynebacterium sp. in infecting Lolium rigidum. Nematologica, 25, 76–85.

    Article  Google Scholar 

  • Riley, I. T. (1992). Anguina tritici is a potential vector of Clavibacter toxicus. Australasian Plant Pathology, 21(4), 147–149.

    Article  Google Scholar 

  • Riley, I. T., Schmitz, A., & Silva, P. (2001). Anguina australis, a vector for Rathayibacter toxicus in Ehrharta longiflora. Australasian Plant Pathology, 30, 171–175.

    Article  Google Scholar 

  • Shina, S., Revathy, K. A., & Bhat, A. I. (2015). Rapid identification of transgenic black pepper using loop-mediated isothermal amplification (LAMP) and real-time LAMP assays. Journal of Plant Biochemistry Biotechnology, 24(4), 466–469.

    Article  Google Scholar 

  • Siddiqi, M. R. (2000). Tylenchida: Parasites of plants and insects (2nd ed.). Oxon: CABI Publishing, CAB International.

    Book  Google Scholar 

  • Song, Q. Q., Wang, L. X., Fang, R., Khan, M. K., Zhou, Y. Q., & Zhao, J. L. (2013). Detection of Mycoplasma wenyonii in cattle and transmission vectors by the loop-mediated isothermal amplification (LAMP) assay. Tropical Animal Health and Production, 45, 247–250.

    Article  PubMed  Google Scholar 

  • Stynes, B. A., & Bird, A. F. (1980). Anguina agrostis, the vector of annual ryegrass toxicity in Australia. Nematologica, 26, 475–490.

    Article  Google Scholar 

  • Subbotin, S. A., Waeyenberge, L., & Moens, M. (2000). Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLPs. Nematology, 2(2), 153–164.

    Article  CAS  Google Scholar 

  • Subbotin, S. A., Krall, E. L., Riley, I. T., Chizhov, V. N., Staelens, A., De Loose, M., & Moens, M. (2004). Evolution of the gall-forming plant parasitic nematodes (Tylenchida:Anguinidae) and their relationships with hosts as inferred from internal transcribed spacer sequences of nuclear ribosomal DNA. Molecular Phylogenetics and Evolution, 30(1), 226–235.

    Article  CAS  PubMed  Google Scholar 

  • Tomlinson, J. A., Boonham, N., & Dickinson, M. (2010). Development of and evaluation of a one hour DNA extraction and loop mediated isothermal amplification assay for rapid detection of phytoplasmas. Plant Pathology, 56, 465–471.

    Article  Google Scholar 

  • Wang, J. C., Ma, Y. G., Zhou, C. N., Du, Y., Huang, G. M., Ding, Y. M., & Xie, H. (2005). Molecular identification bent grass nematode, by the single juvenile. Plant Quarantine, 19(2), 84–86 (in Chinese).

    Google Scholar 

  • Xie, H. (2005). Taxonomy of plant nematodes (Second ed.). Beijing: Higher Education Press.

    Google Scholar 

  • Zhang, J. H., Feng, Y. J., Hu, D., Lv, H., Zhu, J., Cao, M., Zheng, F., Zhu, J., Gong, X. F., Hao, L. N., Srinivas, S., Ren, H., Qi, Z. T., Li, B. J., & Wang, C. J. (2013). Rapid and sensitive detection of H7N9 avian influenza virus by use of reverse transcription–loop-mediated isothermal amplification. Journal of Clinical Microbiology, 51(11), 3760–3764.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhou, Q. J., Cai, Y., Gu, J. F., Wang, X., & Chen, J. (2016). Rapid and sensitive detection of Meloidogyne mali by loop-mediated isothermal amplification combined with a lateral flow dipstick. European Journal of Plant Pathology. doi:10.1007/s10658-016-1130-4.

Download references

Acknowledgements

The project was supported by the Scientific Research Project of Shanghai Entry -Exit Inspection and Quarantine Bureau (No. HK004-2014 and No. HK003-2017).

Author information

Authors and Affiliations

  1. Technical Center for Animal Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau, No.1208, Minsheng Road, Pudong New Area, Shanghai, 200135, People’s Republic of China

    Lu-zhen Yu, Shao-yi Song, Cui Yu, Long-jun Qi, Zi-xiang Yu, Bin-bin Jiao & Juan Yang

Authors
  1. Lu-zhen Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shao-yi Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cui Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Long-jun Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zi-xiang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bin-bin Jiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Juan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shao-yi Song.

Ethics declarations

Ethical statement

The manuscript has not been submitted to more than one journal for simultaneous consideration.

The manuscript has not been published previously (partly or in full).

A single study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time (e.g. “salami-publishing”).

No data have been fabricated or manipulated (including images) to support your conclusions.

No data, text, or theories by others are presented as if they were the author’s own (“plagiarism”). Proper acknowledgements to other works must be given (this includes material that is closely copied (near verbatim), summarized and/or paraphrased), quotation marks are used for verbatim copying of material, and permissions are secured for material that is copyrighted.

Important note: the journal may use software to screen for plagiarism.

Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities - tacitly or explicitly - at the institute/organization where the work has been carried out, before the work is submitted.

Authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

Authors are strongly advised to ensure the correct author group, corresponding author, and order of authors at submission. Changes of authorship or in the order of authors are not accepted after acceptance of a manuscript.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yu, Lz., Song, Sy., Yu, C. et al. A loop mediated isothermal amplification (LAMP) assay for rapid and reliable detection of Anguina wevelli, a grass parasitic nematode. Eur J Plant Pathol 150, 725–734 (2018). https://doi.org/10.1007/s10658-017-1320-8

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10658-017-1320-8

Keywords

Search

Navigation