Abstract
Anguina agrostis is an important grass parasitic nematode and listed as an agricultural and quarantine pest in various countries. It is difficult to identify it based simply on morphology. A loop-mediated isothermal amplification (LAMP) assay was developed to detect A. agrostis. The amplification process of this LAMP method took 60 min, and the detection limit was 1/400 of the total DNA extracted from a single juvenile (J2), an equivalent of 3.15 ng of DNA. This is the first report of the detection of A. agrostis by using a LAMP-based method.
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Acknowledgments
The project was supported by the National Key R&D Program of China (2017YFC1200600), Scientific Research Project of Shanghai Entry -Exit Inspection and Quarantine Bureau (No. HK004-2014 and No. HK003-2017).
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Yu, Lz., Song, Sy., Yu, C. et al. Rapid detection of Anguina agrostis by loop-mediated isothermal amplification. Eur J Plant Pathol 156, 819–825 (2020). https://doi.org/10.1007/s10658-020-01932-y
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DOI: https://doi.org/10.1007/s10658-020-01932-y