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Histopathology of the infection on resistant and susceptible lentil accessions by two contrasting pathotypes of Fusarium oxysporum f.sp. lentis

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Abstract

Wilt disease of lentil caused by Fusarium oxysporum f.sp. lentis (Fol) is one of the most important diseases affecting lentil worldwide. Differential response of six lentil accessions with reported differences in the level of resistance to Fol was studied micro and macroscopically. Penetration took place through root epidermal cells without formation of any specific structure. Hyphae reached the stele within two days after inoculation (dai) and subsequently invaded xylem bundles having colonised the endodermis, vascular system and even vascular parenchyma phloem already by 4 dai. Resistance was observed as a quantitative trait in all studied accessions resulting from varying levels of xylem occlusion with gum-like substances and of degree of colonization observed only after 4 dai. An indication of a qualitative resistance was detected in accession BGE019696 inoculated with pathotype 1 as a fast secretion of phenolic compounds at 4 dai. Plasmolysis of cytoplasm, lignification and accumulation of phenolic compounds, gum-like substances and/or tyloses were observed from 15 to 30 dai. As a result of the various operative mechanisms, significantly lower numbers of propagules were recovered from roots by 15 dai, and a retardation of disease was measured as lower disease index by 30 dai in plants inoculated with pathotype 1, but not in those inoculated with pathotype 7.

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Acknowledgments

This research was carried out based on PhD educational mission No. 3972/200-28/1/1389 of first author from Agricultural Research, Education and Extension Organization (AREEO) of Iran and by project AGL2014-52871 co-financed by FEDER. The authors are grateful to M. Bani, ENSB, Algeria and N. Rispail, CSIC, Spain for advice and technical assistance.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain

    Hamid Reza Pouralibaba & Diego Rubiales

  2. Dryland Agricultural Research Institute of Iran, Maragheh, 119, Iran

    Hamid Reza Pouralibaba

  3. IFAPA Centro Alameda Del Obispo, Avda. Menéndez Pidal s/n, 14004, Córdoba, Spain

    Alejandro Pérez-de-Luque

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  1. Hamid Reza Pouralibaba
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  2. Alejandro Pérez-de-Luque
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  3. Diego Rubiales
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Correspondence to Diego Rubiales.

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Pouralibaba, H.R., Pérez-de-Luque, A. & Rubiales, D. Histopathology of the infection on resistant and susceptible lentil accessions by two contrasting pathotypes of Fusarium oxysporum f.sp. lentis . Eur J Plant Pathol 148, 53–63 (2017). https://doi.org/10.1007/s10658-016-1068-6

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  • DOI: https://doi.org/10.1007/s10658-016-1068-6

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