Abstract
Past studies have shown that the major source of infection of young grapevine plants by Botryosphaeriaceae fungi were through the use of infected rootstock and scion cuttings. To investigate the potential infection pathways of Botryosphaeriaceae species within a rootstock mother vine, three genotyping studies using universally-primed polymerase chain reaction (UP-PCR) were conducted with two Neofusicoccum species, namely N. luteum and N. parvum. The investigations identified genotypes of the fungal isolates in trunk and shoot infections of the same mother vine. Results showed that the trunk and shoot isolates from the same vine were of the same or different genotypes, suggesting multiple infections from different inoculum sources. This study further showed that the Neofusicoccum isolates recovered from the surfaces of the cuttings were of the same or different genotypes from those isolated from adjacent internal tissues, again suggesting multiple sources of external inoculum. Investigations into the spatial distribution of Botryosphaeriaceae fungi within an entire dormant cane also showed that multiple species and genotypes were distributed along the cane but most isolates were sited within the bark, being less frequently in the wood, which suggested that they were latent on surface tissues. Since some adjacent wood and bark infections were caused by the same genotypes, this indicated that wood infection may have originated from the bark. These fungi appear to cause latent infections in the bark of dormant cuttings which are used in plant propagation, thus providing an additional infection pathway for a disease that is known to show obvious symptoms only in older vineyards.
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References
Alves, A., Phillips, A. J. L., Henriques, I., & Correia, A. (2005). Evaluation of amplified ribosomal DNA restriction analysis as a method for the identification of Botryosphaeria species. FEMS Microbiology Letters, 245(2), 221–229.
Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2009). Rainwater dispersal of Botryosphaeria conidia from infected grapevines. New Zealand Plant Protection, 62, 228–233.
Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2011). Identification, potential inoculum sources and pathogenicity of botryosphaeriaceous species associated with grapevine dieback disease in New Zealand. European Journal of Plant Pathology, 131(3), 467–482.
Amponsah, N. T., Jones, E., Ridgway, H. J., & Jaspers, M. V. (2012a). Evaluation of fungicides for the management of Botryosphaeria dieback diseases of grapevines. Pest Management Science, 68(5), 676–683.
Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2012b). Microscopy of some interactions between Botryosphaeriaceae species and grapevine tissues. Australasian Plant Pathology, 41(6), 665–673.
Amponsah, N. T., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2014). Factors affecting Neofusicoccum luteum infection and disease progression in grapevines. Australasian Plant Pathology Society. doi:10.1007/s13313-014-0294-7.
Aroca, A., Gramaje, D., Armengol, J., García-Jiménez, J., & Raposo, R. (2010). Evaluation of the grapevine nursery propagation process as a source of Phaeoacremonium spp. and Phaeomoniella chlamydospora and occurrence of trunk disease pathogens in rootstock mother vines in Spain. European Journal of Plant Pathology, 126(2), 165–174.
Baskarathevan, J., Jaspers, M. V., Jones, E. E., & Ridgway, H. J. (2012a). Incidence and distribution of botryosphaeriaceous species in New Zealand vineyards. European Journal of Plant Pathology, 132(4), 549–560.
Baskarathevan, J., Jaspers, M. V., Jones, E. E., Cruickshank, R. H., & Ridgway, H. J. (2012b). Genetic and pathogenic diversity of Neofusicoccum parvum in New Zealand vineyards. Fungal Biology, 116(2), 276–288.
Baskarathevan, J., Jaspers, M. V., Jones, E. E., & Ridgway, H. J. (2013). Development of isolate-specific markers for Neofusicoccum parvum and N. luteum and their use to study rainwater splash dispersal in the vineyard. Plant Pathology, 62(3), 501–509.
Bihon, W., Burgess, T., Slippers, B., Wingfield, M. J., & Wingfield, B. D. (2011). Distribution of D. pinea and its genotypic diversity within asymptomatic Pinus patula trees. Australasian Plant Pathology, 40(5), 540–548.
Billones-Baaijens, R., Jones, E. E., Ridgway, H. J., & Jaspers, M. V. (2013a). Virulence affected by assay parameters during grapevine pathogenicity studies with Botryosphaeriaceae nursery isolates. Plant Pathology, 62(6), 1214–1225.
Billones-Baaijens, R., Ridgway, H. J., Jones, E. E., & Jaspers, M. V. (2013b). Inoculum sources of Botryosphaeriaceae species in New Zealand nurseries. European Journal of Plant Pathology, 135(1), 159–174.
Billones-Baaijens, R., Ridgway, H. J., Jones, E. E., Cruickshank, R. H., & Jaspers, M. V. (2013c). Prevalence and distribution of Botryosphaeriaceae species in New Zealand grapevine nurseries. European Journal of Plant Pathology, 135(1), 175–185.
Bulat, S. A., Mironenko, N., Lapteva, M. N., & Strelchenko, P. P. (1994). Polymerase chain reaction with universal primers (UP-PCR) and its applications to plant genome analysis. In R. P. Adams, J. S. Miller, E. M. Golenberg, & J. E. Adams (Eds.), Conservation of Plant Genes II: Utilization of ancient and modern DNA (pp. 113–129). Saint Louis: Missouri Botanical Garden.
Dakin, N., White, D., Hardy, G. E. S. J., & Burgess, T. I. (2010). The opportunistic pathogen, Neofusicoccum australe, is responsible for crown dieback of peppermint (Agonis flexuosa) in Western Australia. Australasian Plant Pathology, 39(2), 202–206.
Edwards, J., Pascoe, I., Salib, S., & Laukart, N. (2004). Phaeomoniella chlamydospora and Phaeoacremonium aleophilum can spread into grapevine canes from trunks of infected mother vines. Phytopathologia Mediterranea, 43(1), 154–155.
Flowers, J., Nuckles, E., Hartman, J., & Vaillancourt, L. (2001). Latent infection of Austrian and Scots pine tissues by Sphaeropsis sapinea. Plant Disease, 85(10), 1107–1112.
Fourie, P. H., & Halleen, F. (2004). Occurrence of grapevine trunk disease pathogens in rootstock mother plants in South Africa. Australasian Plant Pathology, 33(2), 313–315.
Giménez-Jaime, A., Aroca, A., Raposo, R., García-Jiménez, J., & Armengol, J. (2006). Occurrence of fungal pathogens associated with grapevine nurseries and the decline of young vines in Spain. Journal of Phytopathology, 154(10), 598–602.
Gramaje, D., Armengol, J., & Ridgway, H. J. (2013). Genetic and virulence diversity, and mating type distribution of Togninia minima causing grapevine trunk diseases in Spain. European Journal of Plant Pathology, 135, 727–743.
Hartill, W. F. T., & Everett, K. R. (2002). Inoculum sources and infection pathways of pathogens causing stem-end rots of 'Hass' avocado (Persea americana). New Zealand Journal of Crop and Horticultural Science, 30(4), 249–260.
Johnson, G. I., Mead, A. J., Cooke, A. W., & Dean, J. R. (1992). Mango stem end rot pathogens - Fruit infection by endophytic colonization of the inflorescence and pedicel. Annals of Applied Biology, 120(2), 225–234.
Kim, K. W., Park, E. W., Kim, Y. H., Ahn, K. K., Kim, P. G., & Kim, K. S. (2001). Latency- and defense-related ultrastructural characteristics of apple fruit tissues infected with Botryosphaeria dothidea. Phytopathology, 91(2), 165–172.
Kim, K. W., Park, E. W., & Kim, K. S. (2004). Glyoxysomal nature of microbodies complexed with lipid globules in Botryosphaeria dothidea. Phytopathology, 94(9), 970–977.
Larignon, P., Fulchic, R., Cere, L., & Dubos, B. (2001). Observation on black dead arm in French vineyards. Phytopathologia Mediterranea, 40, S336–S342. Supplement.
Larignon, P., Coarer, M., Larbre, C., Girardon, K., Viguès, V., & Yobregat, O. (2009). Maladies du bois de la vigne, côté pépinières: Identification sur le matériel végétal des sources d’inoculum des champignons associés à ces maladies. Phytoma. 622-623, 46-48.
Lübeck, M., Alekhina, I. A., Lübeck, P. S., Jensen, D. F., & Bulat, S. A. (1999). Delineation of Trichoderma harzianum into two different genotypic groups by a highly robust fingerprinting method, UP-PCR, and UP-PCR product cross-hybridization. Mycological Research, 103(3), 289–298.
Mostert, L., Abeln, E. C. A., Halleen, F., & Crous, P. W. (2006). Genetic diversity among isolates of Phaeomoniella chlamydospora on grapevines. Australasian Plant Pathology, 35, 453–460.
Pascoe, I., & Cottral, E. (2000). Developments in grapevine trunk diseases research in Australia. Phytopathologia Mediterranea, 39(1), 68–75.
Pathrose, B., Jones, E. E., Jaspers, M. V., & Ridgway, H. J. (2014). (2014). High genotypic and virulence diversity in Ilyonectria liriodendri isolates associated with black foot disease in New Zealand vineyards. Plant Pathology, 63, 613–624.
Phillips, A. J. L. (1998). Botryosphaeria dothidea and other fungi associated with excoriose and dieback of grapevines in Portugal. Journal of Phytopathology, 146(7), 327–332.
Phillips, A. J. L. (2002). Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathologia Mediterranea, 41(1), 3–18.
Pusey, P. L. (1993). Role of Botryosphaeria species in peach tree gummosis on the basis of differential isolation from outer and inner bark. Plant Disease, 77(2), 170–174.
Sakalidis, M. L., Hardy, G. E. S. J., & Burgess, T. I. (2011). Class III endophytes, clandestine movement amongst hosts and habitats and their potential for disease; a focus on Neofusicoccum australe. Australasian Plant Pathology, 40(5), 510–521.
Slippers, B., & Wingfield, M. J. (2007). Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biology Reviews, 21(2–3), 90–106.
Smith, H., Wingfied, M. J., & Coutinho, T. A. (2002). The role of latent Sphaeropsis sapinea infections in post-hail associated die-back of Pinus patula. Forest Ecology and Management, 164(1–3), 177–184.
Úrbez-Torres, J. R., & Gubler, W. D. (2009). Pathogenicity of Botryosphaeriaceae species isolated from grapevine cankers in California. Plant Disease, 93(6), 584–592.
Urbez-Torres, J. R., & Gubler, W. D. (2011). Susceptibility of grapevine pruning wounds to infection by Lasiodiplodia theobromae and Neofusicoccum parvum. Plant Pathology, 60(2), 261–270.
Úrbez-Torres, J. R., Battany, M., Bettiga, L. J., Gispert, C., McGourty, G., Roncoroni, J., et al. (2010). Botryosphaeriaceae Species Spore-Trapping Studies in California Vineyards. Plant Disease, 94(6), 717–724.
Van Niekerk, J. M., Calitz, F. J., Halleen, F., & Fourie, P. H. (2010). Temporal spore dispersal patterns of grapevine trunk pathogens in South Africa. European Journal of Plant Pathology, 127(3), 375–390.
Whiteman, S.A. (2004). Phaeomoniella chlamydospora: potential inoculum sources in the grapevine propagation process. Lincoln University, Lincoln, New Zealand, PhD Thesis.
Whiteman, S. A., Stewart, A., Ridgway, H. J., & Jaspers, M. V. (2007). Infection of rootstock mother-vines by Phaeomoniella chlamydospora results in infected young grapevines. Australasian Plant Pathology, 36(2), 198–203.
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22), 6531–6535.
Acknowledgments
We thank Winegrowers New Zealand and Lincoln University for funding this research and Corban’s Viticulture Ltd. for allowing for us to conduct our research in their nursery.
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Billones-Baaijens, R., Ridgway, H.J., Jones, E.E. et al. Spatial distribution of Neofusicoccum species within a rootstock mother vine indicates potential infection pathways. Eur J Plant Pathol 141, 267–279 (2015). https://doi.org/10.1007/s10658-014-0540-4
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DOI: https://doi.org/10.1007/s10658-014-0540-4