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The role of jasmonic acid signalling in wheat (Triticum aestivum L.) powdery mildew resistance reaction

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Abstract

Jasmonic acid (JA) signalling plays an important role in plant resistance to pathogens. Previously, JA has been found to play a role in induced disease resistance to necrotrophic pathogens in various plant species, but current researches showed that JA also enhanced resistance to biotrophic pathogens. However, its role in wheat (Triticum aestivum L.) powdery mildew (Blumeria graminis f. sp. tritici, Bgt) resistance reaction is largely unknown. To settle this issue, several typical powdery mildew resistant and susceptible wheat varieties were employed. The sensitivity to exogenous methyl jasmonate (MeJA) to wheat powdery mildew resistance, the concentration fluctuation of endogenous JAs after Bgt inoculation, and the expression profiles of nine pathogenesis-related protein genes (PR genes) after MeJA and Bgt treatments were studied systematically. Exogenous MeJA significantly enhanced the powdery mildew resistance of the susceptible varieties. After inoculation with Bgt, endogenous JAs accumulated rapidly, reached the maxima at 2 to 5 h post-inoculation (hpi), then decreased rapidly, and the concentration was almost the same as that of un-inoculated control at 96 hpi. The expression levels of the nine PRs were measured by real time quantitative RT-PCR (qRT-PCR) at different time points after MeJA application or Bgt inoculation respectively. The MeJA and Bgt strongly activated PR1, PR2, PR3, PR4, PR5, PR9, PR10 and Ta-JA2, but almost didn’t affect Ta-GLP2a. The induced powdery mildew resistance was positively correlated with the activated PR genes. JA plays a positive role in defence against Bgt. JA is a signalling molecule in wheat powdery mildew resistance and future manipulation of this pathway may improve powdery mildew resistance in wheat breeding.

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Acknowledgment

This project was supported by National 863 program (2012AA101105) and Henan key scientific and technological project (122101110200). We are grateful to the anonymous reviewers for their constructive comments on revision of the manuscript.

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Authors and Affiliations

  1. National Center of Engineering and Technological Research for Wheat, Henan Agricultural University/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou, 450002, China

    Zongbiao Duan, Chuncai Shen, Qiaoyun Li & Jishan Niu

  2. College of life sciences, Henan Agricultural University, Zhengzhou, Henan, China

    Guizhen Lv & Zhao Qin

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  1. Zongbiao Duan
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  2. Guizhen Lv
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  6. Jishan Niu
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Correspondence to Jishan Niu.

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Duan, Z., Lv, G., Shen, C. et al. The role of jasmonic acid signalling in wheat (Triticum aestivum L.) powdery mildew resistance reaction. Eur J Plant Pathol 140, 169–183 (2014). https://doi.org/10.1007/s10658-014-0453-2

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