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Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response

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Abstract

The responses to cellulysin as an immune inducer in pearl millet that confers downy mildew resistance mediated through lipoxygenase (LOX), a jasmonate-dependent enzyme involved in defence signalling, are discussed in this paper. The susceptible pearl millet cultivar 7042S was treated with cellulysin at 10, 15, 20, 30 and 50 μg/ml concentrations. All tested concentrations showed enhanced seed germination and seedling vigour when compared with the untreated control. Maximum seed germination of 92 % and seedling vigour was obtained following 20 μg/ml cellulysin treatment. Significant (P < 0.05) downy mildew disease protection of 67 % and 71 % was observed when cellulysin was used at 20 μg/ml under greenhouse and field conditions, respectively. Further studies showed that the resistance induced by cellulysin treatment in pearl millet plant was systemic, required a minimum of 4 days to achieve maximum resistance development after pathogen inoculation seedling inoculation (five-day-old), and was sustained throughout the plant’s life. Plants raised from cellulysin-treated seeds and challenge inoculated at tillering (25-day-old) and inflorescence (45-day-old) showed persistence in resistance till the end of the crop period. A notable increase in LOX activity was observed in all the tested concentrations of cellulysin in plants inoculated with the pathogen at 24 h, compared to the control. However, a maximum 6-fold increase in LOX activity was noticed using a cellulysin concentration of 20 μg/ml 48 hours post inoculation. In contrast, glucanase (GLU) activity was high in control inoculated seedlings, but was low in cellulysin treated samples at all time intervals. The optimal cellulysin treatment (20 μg/ml) provided enhanced vegetative and reproductive parameters that resulted in higher yield compared to the untreated control.

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Acknowledgements

The authors thank Indian Council of Agricultural Research (ICAR), the Government of India through All India Coordinated Pearl Millet Improvement Program (AICPMIP) for laboratory and field facilities at department of Biotechnology, University of Mysore, Karnataka State, India.

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Authors and Affiliations

  1. Downy Mildew Research Laboratory (DMRL), Department of Biotechnology, University of Mysore, Manasagangothri, Mysore, 570 006, Karnataka, India

    H. G. Pushpalatha, J. Sudisha & H. Shekar Shetty

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  1. H. G. Pushpalatha
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  2. J. Sudisha
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  3. H. Shekar Shetty
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Correspondence to H. Shekar Shetty.

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H. G. Pushpalatha and J. Sudisha Equal contribution in this research investigation.

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Pushpalatha, H.G., Sudisha, J. & Shetty, H.S. Cellulysin induces downy mildew disease resistance in pearl millet driven through defense response. Eur J Plant Pathol 137, 707–717 (2013). https://doi.org/10.1007/s10658-013-0281-9

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