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Oxylipins are not required for R gene-mediated resistance in potato

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Abstract

The role of 9- and 13-lipoxygenase-derived oxylipins for race-cultivar-specific resistance in potato was analyzed by expressing RNA interference constructs against oxylipin biosynthetic genes in transgenic potato plants carrying the resistance gene R1 against Phytophthora infestans. Down-regulation of 9-lipoxygenase expression resulted in highly reduced levels of 9-hydroxyoctadecatrienoic acid after treatment with the pathogen-associated molecular pattern Pep-13. However, neither 9-lipoxygenase nor 9-divinyl ether synthase RNAi plants exhibited alterations in their resistance to P. infestans. Similarly, successful down-regulation of transcript accumulation of the 13-lipoxygenase pathway genes encoding allene oxide cyclase, 12-oxophytodienoic acid reductase 3 and the jasmonic acid receptor coronatine-insensitive 1 resulted in highly reduced levels of jasmonic acid after Pep-13 treatment. Race-cultivar-specific resistance, however, was not lost in these plants. Our results suggest that neither 9-lipoxygenase-derived oxylipins nor jasmonic acid are required for R-gene-mediated resistance in potato. Importantly, in tobacco, the silencing of 9-lipoxygenase expression was previously demonstrated to suppress race-cultivar-specific resistance. Thus, we conclude a differential requirement of oxylipins for R-gene-mediated resistance in different solanaceous plants.

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Fig. 1
Fig. 2

Abbreviations

9-HOT:

9-hydroxyoctadecatrienoic acid

JA:

Jasmonic acid

LOX:

Lipoxygenase

NtLOX1 :

Nicotiana tabacum 9-lipoxygenase 1

PAMP:

Pathogen-associated molecular pattern

Ppn :

Phytophthora parasitica var. nicotianae

R :

Resistance gene

StAOC :

Solanum tuberosum allene oxide cyclase

StCOI1 :

S. tuberosum coronatine-insensitive 1

StLOX1 :

S. tuberosum 9-lipoxygenase 1

StDES :

S. tuberosum 9-divinyl ether synthase

StOPR3 :

S. tuberosum 12-oxophytodienoic acid reductase 3

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Acknowledgements

The excellent technical assistance of Angelika Weinel and Ulrike Smolka is gratefully acknowledged. We thank Prof. Felix Mauch for providing P. infestans 208m2, Sabine Freitag for help with 9-HOT analysis and Birgit Ortel for help with JA measurements. This work was funded by the Deutsche Forschungsgemeinschaft (SFB 648, TP A4).

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Author notes

  1. Simone Altmann

    Present address: Department of Disease and Stress Biology, John Innes Centre, Norwich, NR4 7UH, UK

Authors and Affiliations

  1. Department Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany

    Lennart Eschen-Lippold, Simone Altmann & Sabine Rosahl

  2. Department for Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Cologne, Germany

    Christiane Gebhardt

  3. Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany

    Cornelia Göbel & Ivo Feussner

Authors
  1. Lennart Eschen-Lippold
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Corresponding author

Correspondence to Sabine Rosahl.

Additional information

Lennart Eschen-Lippold and Simone Altmann contributed equally to the work.

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Eschen-Lippold, L., Altmann, S., Gebhardt, C. et al. Oxylipins are not required for R gene-mediated resistance in potato. Eur J Plant Pathol 127, 437–442 (2010). https://doi.org/10.1007/s10658-010-9621-1

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