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Efficacy of olive mill waste water and its derivatives in the suppression of crown gall disease of bitter almond

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Abstract

Olive mill waste water (OMW) and some of its indigenous bacterial strains were tested in vitro and in planta for their efficacy against crown gall disease caused by Agrobacterium tumefaciens. OMW and polyphenols displayed a high level of antibacterial activity, however the volatile fraction was less efficient as only a bacteriostatic effect was observed. In pot experiments, the percentage of bitter almond rootstock showing symptoms of crown gall was significantly reduced with the dosage rate of OMW 1% as compared to the control (highly natural infected soil treated with water). Five indigenous bacterial strains isolated from OMW exhibited an antagonistic effect against the bacterium. Based on the gene 16S rRNA sequence analysis, one isolate showed 99.2% similarity to known sequences of Bacillus subtilis, one isolate demonstrated high percentage similarities (99.3%) to the genera Bacillus pumilis, and two isolates were associated with Stenotrophomonas maltophilia and Pseudomonas putida 100% and 99.6% similarities respectively. Among these bacteria, the strain B1 proved efficient against the soil borne pathogen in vitro and pot experiments. Our study in controlled conditions suggested that the addition of OMW to soil exerts significant disease suppressiveness against A. tumefaciens.

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Acknowledgements

This work was supported by the funds of the project CFC/IOOC/04. We would like to thank Dr. Xavier Nesme from Laboratoire d’Ecologie Microbienne (Université Claude Bernard, France) for his contribution in the identification of the bacterial strains.

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Authors and Affiliations

  1. Laboratoire Eau, Energie et Environnement, Ecole Nationale des Ingénieurs Sfax Tunisia, Rte de Soukra Km 4.5, 3038, Sfax, Tunisia

    Thabèt Yangui & Jalel Bouzid

  2. Unité de recherche Protection des Plantes Cultivées et Environnement, Institut de l’olivier de Sfax, Rte de Soukra Km 1.5, 3003, Sfax, Tunisia

    Ali Rhouma & Mohamed Ali Triki

  3. Laboratoire de recherche Amélioration de la Productivité de l’Olivier et des Arbres fruitiers, Institut de l’Olivier, BP 1087, 3000, Sfax, Tunisia

    Kamel Gargouri

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  1. Thabèt Yangui
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  2. Ali Rhouma
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  3. Kamel Gargouri
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  4. Mohamed Ali Triki
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  5. Jalel Bouzid
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Correspondence to Ali Rhouma.

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Thabet Yangui and Ali Rhouma contributed equally to this work and are regarded as joint first authors.

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Yangui, T., Rhouma, A., Gargouri, K. et al. Efficacy of olive mill waste water and its derivatives in the suppression of crown gall disease of bitter almond. Eur J Plant Pathol 122, 495–504 (2008). https://doi.org/10.1007/s10658-008-9317-y

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  • DOI: https://doi.org/10.1007/s10658-008-9317-y

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