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Effect of roundup® (glyphosate formulation) in the energy metabolism and reproductive traits of Hyalella castroi (Crustacea, Amphipoda, Dogielinotidae)

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Abstract

Roundup® (glyphosate formulation) is a nonselective and posts emergent herbicide used for controlling aquatic weeds and different concentrations are used in cultures around the world. The objective of this investigation was to examine the effects of Roundup® (glyphosate formulation) on the biochemical composition, levels of lipoperoxidation, Na+/K+ATPase activity and reproductive traits in the Hyalella castroi. Amphipods were collected in summer 2009, in the southern Brazilian highlands. In the laboratory, the animals were kept in aquariums under controlled conditions for 7 days, and after this period they were exposed to 0.36, 0.52, 1.08 and 2.16 mg/l of glyphosate for 7 days. After the period of exposure, the animals were immediately frozen for determination of glycogen, proteins, lipids, triglycerides, cholesterol, levels of lipoperoxidation, and Na+/K+ATPase activity. During each day of the cultivation reproductive traits (number of reproductive pairs, ovigerous females and eggs in the marsupium) were observed. All concentrations of Roundup® induced significant decreases in all biochemical parameters and Na+/K+ATPase activity, and significant increase in lipoperoxidation levels. Showing this form a potentially toxic effect at very low concentrations, this pattern of results can lead to significant changes in trophic structure of limnic environments because these amphipods are important links in food chain in these habitats.

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Acknowledgments

The authors wish to express their gratitude to the reviewer for the valorous suggestion of change in this work. The current stuffy was supported by the CAPES/MINCYT (Brazil-Argentina) for the financial support to Dr. Guendalina Turcato Oliveira (Project 50/03) and by fellowship give by Conselho Nacional de Desenvolvimento Tecnológico (CNPq, Process no. 140283/2008-0) to the first author. This work was carried out according to state and federal laws concerning wild animal sampling.

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Authors and Affiliations

  1. Departamento de Ciências Morfofisiológicas, Laboratório de Fisiologia da Conservação - Programa de Pós-graduação em Zoologia - Faculdade de Biociências da Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Av. Ipiranga, 6681 Pd. 12A Sala 270, Caixa Postal 1429, Porto Alegre, RS, 90619-900, Brazil

    Bibiana Kaiser Dutra, Felipe Amorim Fernandes, Daniela Motta Failace & Guendalina Turcato Oliveira

  2. Bolsista de Produtividade do CNPq, Sao Paulo, Brazil

    Guendalina Turcato Oliveira

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  1. Bibiana Kaiser Dutra
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  2. Felipe Amorim Fernandes
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  3. Daniela Motta Failace
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  4. Guendalina Turcato Oliveira
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Correspondence to Guendalina Turcato Oliveira.

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Dutra, B.K., Fernandes, F.A., Failace, D.M. et al. Effect of roundup® (glyphosate formulation) in the energy metabolism and reproductive traits of Hyalella castroi (Crustacea, Amphipoda, Dogielinotidae). Ecotoxicology 20, 255–263 (2011). https://doi.org/10.1007/s10646-010-0577-x

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