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Fluroxypyr triggers oxidative damage by producing superoxide and hydrogen peroxide in rice (Oryza sativa)

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Abstract

The present study has evaluated effect of fluroxypyr concentrations 0–0.8 mg l−1 (a widely-used herbicide for controlling annual or perennial weeds growth) on selected metabolic and stress-related parameters in Oryza sativa plants after 6 days of exposure. Increasing concentrations decreased shoot growth and accumulation of chlorophylls but had no effect on root biomass. Increasing doses led also to increase in superoxide radical, hydrogen peroxide and proline accumulation, while malondialdehyde, an indicator of lipid peroxidation, was constitutively elevated. Histochemical staining with nitroblue tetrazolium and 3, 3-diaminobenzidine were positively correlated with the generation of superoxide radical and H2O2. The fluroxypyr-induced oxidative stress triggered significant changes in activities of superoxide dismutase, catalase, ascorbate peroxidase and peroxidase (POD). Activities of the antioxidant enzymes show a general increase at low fluroxypyr concentrations and a decrease at high fluroxypyr levels (except for POD). Analysis of naturing polyacrylamide gel electrophoresis confirmed these results. These data support the observation that fluroxypyr-triggered oxidative stress was responsible for the disturbance of the growth in the rice plants.

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (No. 20777037).

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Authors and Affiliations

  1. Department of Applied Chemistry, College of Science, Nanjing Agricultural University, Weigang, Outside the Zhongshan Men, Building of Chemistry, 210095, Nanjing, China

    Guo Lin Wu, Jing Cui, Ling Tao & Hong Yang

  2. Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing Agricultural University, Weigang, Outside the Zhongshan Men, Building of Chemistry, 210095, Nanjing, China

    Guo Lin Wu, Ling Tao & Hong Yang

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  1. Guo Lin Wu
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  2. Jing Cui
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  3. Ling Tao
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  4. Hong Yang
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Correspondence to Hong Yang.

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Wu, G.L., Cui, J., Tao, L. et al. Fluroxypyr triggers oxidative damage by producing superoxide and hydrogen peroxide in rice (Oryza sativa). Ecotoxicology 19, 124–132 (2010). https://doi.org/10.1007/s10646-009-0396-0

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  • DOI: https://doi.org/10.1007/s10646-009-0396-0

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