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Comparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China

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Abstract

To obtain the phylogenetic relationship between diploid and tetraploid Misgurnus anguillicaudatus, the mitochondrial cyt b gene in the diploid and tetraploid weather loach were isolated and sequenced. The DNA sequences were analyzed using MEGA 3.0 software to determine the phylogenetic relationship. Forty-five variable sites among cyt b gene sequences and 18 amino acid substitutions occurred within the diploid and tetraploid loaches as deduced from the nucleotide sequences analysis of the cyt b gene. The nucleotide pairwise distance between diploid and tetraploid loach ranged from 0.001 to 0.025. Phylogenetic analysis revealed evolutionary relationships between diploid and tetraploid loach. Our results indicated a significant difference between diploid and tetraploid loach about the cyt b gene. AMOVA analysis indicated that there were no significant genetic variations within diploid loaches (Fst = 0.2529, P > 0.05) and within tetraploid loaches (Fst = 0.0564, P > 0.05), neither. However, significant genetic differences were found between diploid and tetraploid loaches (Fst = 0.7634, P < 0.05). Thus, it is concluded that no reproductive isolation was found within the same cytotypes of different localities, but there was reproductive isolation between these two cytotypes. The diploid loach existed before the tetraploid loach in nature. The present study is the first to describe the phylogenetic relationships of natural polyploidy weather loach using mtDNA cyt b gene.

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Acknowledgement

This research is a component of the Aquaculture Collaborative Research Support Program (ACRSP), supported by the US Agency for International Development Grant, and by contributions from University of Michigan, Asian Institute of Technology, and Huazhong Agricultural University. The accession number is 1329. The opinions expressed herein are those of the authors and do not necessarily reflect the views of the US Agency for International Development. This study is also funded by “National Key Technology R&D Program in Eleventh-Five-Year Plan” titled as “The integrated demonstration of safe fishery production”. The accession number is 2006BAK02A22.

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Authors and Affiliations

  1. College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Chengtai Yang, Ling Cao, Weimin Wang, Khalid Abbas, Yuhua Sun & Huanling Wang

  2. Aquaculture and Aquatic Resources Management, School of Environment, Resources and Development, Asian Institute of Technology, P.O. Box 4, Klong Luang, Pathum Thani, 12120, Thailand

    Yi Yang

  3. Jiangsu Key Lab of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, Jiangsu, 222005, People’s Republic of China

    Binlun Yan

  4. Aquaculture Genetics and Breeding Laboratory, Ohio State University South Centers, 1864 Shyville Road, Piketon, OH, 45661, USA

    Hanping Wang

  5. Huangshi Institute of Technology, Huangshi, Hubei, 435003, People’s Republic of China

    Li Su

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  1. Chengtai Yang
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Correspondence to Weimin Wang.

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Yang, C., Cao, L., Wang, W. et al. Comparative and evolutionary analysis in natural diploid and tetraploid weather loach Misgurnus anguillicaudatus based on cytochrome b sequence data in central China. Environ Biol Fish 86, 145–153 (2009). https://doi.org/10.1007/s10641-007-9283-9

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