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Effects of Bifidobacterium infantis 35624 on Post-Inflammatory Visceral Hypersensitivity in the Rat

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Abstract

Background

Irritable bowel syndrome patients have abnormal visceral perception. Probiotic organisms may produce beneficial effects in these patients by reducing visceral hypersensitivity.

Aim

To investigate the effects of the probiotic organism, Bifidobacterium infantis 35624, on post-inflammatory visceral hypersensitivity in rats.

Methods

Colitis was induced using intracolonic administration of trinitrobenzenesulfonic acid; control rats received saline (day 0). Myeloperoxidase (MPO) levels and colonic damage scores were determined. From days 15–29, rats (n = 10/group) rats were orally dosed with 2 ml of B. infantis ≥ 108 colony-forming units/ml or vehicle (MRS broth). A second series of rats (n = 10/group) was dosed in the same manner from days 15–59. The level of colonic stimulation during colorectal distension (CRD) was determined by recording a visceromotor response (VMR) to CRD at 30 mmHg pre- and post-treatment. Post-treatment samples of colonic tissue were weighed, graded for morphologic damage, and assayed for MPO levels.

Results

All rats were hypersensitive at day 15. On day 30, hypersensitivity to colorectal distension remained in the vehicle group, but was significantly reduced in the B. infantis group (mean VMR/10 min: vehicle = 15.4 ± 1.0 vs. B. infantis = 7.6 ± 1.0, p < 0.001). A similar, significant effect was observed at day 60. On both day 30 and day 60, tissue weight, colonic damage scores, and MPO levels resembled those of control animals.

Conclusions

Oral administration of Bifidobacterium infantis 35624 normalized sensitivity to colorectal distension in a rat model of post-inflammatory colonic hypersensitivity.

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Acknowledgments

This study was supported by a research grant from the Procter & Gamble Company.

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Authors and Affiliations

  1. Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    Anthony C. Johnson & Beverley Greenwood-Van Meerveld

  2. Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    Beverley Greenwood-Van Meerveld

  3. The Procter and Gamble Company, 8700 Mason Montgomery Road, Mason, OH, 45040, USA

    John McRorie

  4. VAMC, Research Administration, Rm. 151, 921 NE 13th St., Oklahoma City, OK, 73104, USA

    Beverley Greenwood-Van Meerveld

Authors
  1. Anthony C. Johnson
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  2. Beverley Greenwood-Van Meerveld
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  3. John McRorie
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Correspondence to Beverley Greenwood-Van Meerveld.

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Johnson, A.C., Greenwood-Van Meerveld, B. & McRorie, J. Effects of Bifidobacterium infantis 35624 on Post-Inflammatory Visceral Hypersensitivity in the Rat. Dig Dis Sci 56, 3179–3186 (2011). https://doi.org/10.1007/s10620-011-1730-y

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  • DOI: https://doi.org/10.1007/s10620-011-1730-y

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