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Circulating Cell-Derived Microparticles in Crohn’s Disease

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Abstract

Procoagulant membrane microparticles can be released from activated or apoptotic cells in response to various environmental stimuli. The aim of this study was to investigate the presence of microparticles in Crohn’s disease and to assess their variations after infliximab therapy. We compared the levels of circulating microparticles in 38 patients with Crohn’s disease, 16 patients with ulcerative colitis, 7 patients with infectious colitis, and 17 control subjects. The evolution of microparticle levels was assessed after infliximab therapy in 13 patients with Crohn’s disease. Circulating microparticle levels were elevated in patients with Crohn’s disease (9.31 ± 0.66 nmol/L phosphatidylserine equivalent [PS Eq]) or infectious colitis (10.71 ± 0.92 nmol/L PS Eq) compared to patients with ulcerative colitis (5.75 ± 0.59 nmol/L PS Eq) and control subjects (4.06 ± 0.37 nmol/L PS Eq) (P = 0.001). Infliximab induced a significant diminution of the amounts of circulating microparticles, from 10.33 ± 1.20 to 6.45 ± 0.90 nmol/L PS Eq (P = 0.002). Generation of circulating microparticles occurs in Crohn’s disease; infliximab induces significant diminution. Release of microparticles could be linked to the type of inflammatory response underlying Crohn’s disease.

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Authors and Affiliations

  1. Service d’Hépato-Gastroentérologie et d’Assistance Nutritive, Université Louis Pasteur, Strasbourg, France

    Patrick Chamouard PhD, MD & René Baumann Pr

  2. Laboratoire d’Hématologie, Hôpital de Hautepierre, Strasbourg, Université Louis Pasteur, Strasbourg, France

    Dominique Desprez MD, Michel Lessard Pr & Lélia Grunebaum MD

  3. Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France

    Bénédicte Hugel PhD, Corinne Kunzelmann PhD, Carole Gidon-Jeangirard PhD & Jean-Marie Freyssinet PhD

  4. Hôpital de Bicêtre, Le Kremlin-Bicêtre, Unité 143 INSERM, France

    Patrick Chamouard PhD, MD, Dominique Desprez MD, Bénédicte Hugel PhD, Corinne Kunzelmann PhD, Carole Gidon-Jeangirard PhD, Michel Lessard Pr, René Baumann Pr, Jean-Marie Freyssinet PhD & Lélia Grunebaum MD

  5. Service d’Hépato-Gastroentérologie, et d’Assistance Nutritive, Hôpital de Hautepierre, Strasbourg Cedex, 67098, France

    Patrick Chamouard PhD, MD

Authors
  1. Patrick Chamouard PhD, MD
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  2. Dominique Desprez MD
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  3. Bénédicte Hugel PhD
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  9. Lélia Grunebaum MD
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Correspondence to Patrick Chamouard PhD, MD.

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Chamouard, P., Desprez, D., Hugel, B. et al. Circulating Cell-Derived Microparticles in Crohn’s Disease. Dig Dis Sci 50, 574–580 (2005). https://doi.org/10.1007/s10620-005-2477-0

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  • DOI: https://doi.org/10.1007/s10620-005-2477-0

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