Abstract
Allophylus cobbe (L.) Raeusch. belonging to the family Sapindaceae, is a commonly distributed small shrub in Western Ghats of India previously reported for its traditional medicinal properties. It is used for the treatment of various ailments. The present study is aimed at investigating preliminary phytochemicals, inducing the determination of the total phenolic contents, antioxidant assays and anticancer activity of A. cobbe leaf extracts on (DU-145) and (PC-3) cell lines. Preliminary phytochemical screening showed a broad spectrum of secondary metabolites. Highest amount of phenolic content was present in aqueous extract (91.96 ± 0.61 mg/g GAE) and it also proved to have the most potent antioxidant activity at a concentration of 100 mg/ml (64.71 ± 0.15%). IC50 value was (431.10 ± 15.05 µg/mL) for DU-145 and (362.08 ± 24.17 µg/mL) for PC-3 cell lines while the standard drug paclitaxel showed an IC50 value of 0.3 µM/mL. Morphological changes was observed in cancerous cells undergoing apoptosis in human prostate cancer cell lines (DU-145 and PC-3) while the extract showed no cytotoxicity towards normal cells (MEF-L929). It can be concluded that the tested extracts holds significant antioxidant and anticancer activities. However further investigation on lead compounds of A. cobbe will enable its therapeutic use.
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Acknowledgements
The authors are grateful to Dr. Kotresha K, Department of Botany, Karnataka Science College, Dharwad, Karnataka, for identifying the plant. The authors express their sincere gratitude to the authorities of Dr. Prabhakar Kore Basic Science Research Center, KLE University, Belagavi, Karnataka, India, for providing the laboratory facilities to execute the soxhlet extraction and cell culture experiments. The authors also wish to sincerely thank Dr. Sandeep R. Pai, Assistant Professor, Amity Institute of Biotechnology, Amity University, Mumbai, for his guidance in execution of phenolic content and antioxidant assays.
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Ghagane, S.C., Puranik, S.I., Nerli, R.B. et al. Evaluation of in vitro antioxidant and anticancer activity of Allophylus cobbe leaf extracts on DU-145 and PC-3 human prostate cancer cell lines. Cytotechnology 69, 167–177 (2017). https://doi.org/10.1007/s10616-016-0048-1
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DOI: https://doi.org/10.1007/s10616-016-0048-1