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Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

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Abstract

Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.

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Abbreviations

ALX:

Alloxan

BSA:

Bovine serum albumin

EDTA:

Ethylenediaminetetraacetic acid

ERW:

Electrolyzed reduced water

FBS:

Fetal bovine serum

DM:

Diabetes mellitus

HBSS:

Hank’s balanced salt solution

HEPES:

4-[2-hydroxyethyl]-1-piperazineethane-sulfonic acid

PBS:

Phosphate buffered saline

PI:

Propidium iodide

ROS:

Reactive oxygen species

T1DM:

Type 1 diabetes mellitus

T2DM:

Type 2 diabetes mellitus

TdT:

Terminal deoxynucleotidyl transferase

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Acknowledgments

The authors thank Ms. Yuki Higuchi for excellent technical assistance.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Yupin Li, Takeki Hamasaki, Taichi Kashiwagi, Takaaki Komatsu, Jun Ye, Kiichiro Teruya, Masumi Abe & Sanetaka Shirahata

  2. School of Life Sciences, Nanchang University of Science and Technology, Nanchang, 330013, People’s Republic of China

    Yupin Li

  3. Nihon Trim Co Ltd, 1-8-34 Oyodonaka, Kita-ku, Osaka, 531-0076, Japan

    Noboru Nakamichi & Shigeru Kabayama

  4. School of Life Science, Xiamen University, Fujian, 361005, People’s Republic of China

    Jun Ye

  5. Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Kiichiro Teruya, Hanxu Yan, Tomoya Kinjo & Sanetaka Shirahata

  6. Kyowa Hospital, 191-1 Sakae, Osibetanimachi, Nishi-ku, Kobe, 851-2211, Japan

    Munenori Kawamura

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  1. Yupin Li
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  2. Takeki Hamasaki
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  3. Noboru Nakamichi
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Correspondence to Sanetaka Shirahata.

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Li, Y., Hamasaki, T., Nakamichi, N. et al. Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus. Cytotechnology 63, 119–131 (2011). https://doi.org/10.1007/s10616-010-9317-6

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  • DOI: https://doi.org/10.1007/s10616-010-9317-6

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