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Measurement of [18F]-fluorodeoxyglucose incorporation into human osteoblast–An experimental method

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Abstract

An evaluation of human osteoblast metabolism usually involves measurements of the by-products of bone matrix elaboration. The assessment of glycolytic activity of osteoblasts is not a standard tool in most of the reports, but might be of value by providing a direct indicator of cellular metabolism. Measurement of the incorporation of [18F]-fluorodeoxyglucose, which is not further degradable following its conversion into glycose-6-phosphate during glycolysis and is trapped in this form within the cells, can be used as an effective research tool for estimation of osteoblast metabolism. In order to estimate the [18F]-fluorodeoxyglucose incorporation we used cultured human osteoblast-like cells. Following incubation of the culture samples in a glucose free medium with 5 μ Ci [18F]-fluorodeoxyglucose we measured the radioactivity of the cell fraction, as a percent from the initial dose, and compared to the incorporation values in cells treated by protoporphyrine IX (10−5 M), an endogenous pro-apoptotic agent. To compare the response of [18F]-fluorodeoxyglucose incorporation studies, following treatment of cells with the protoporphyrine IX, to other experimental cell metabolism evaluation methods, we performed a parallel comparison of alkaline phospatase activity, which is a standard measurement tool of osteoblast metabolism, in the control and treatment groups. A narrow range of 0.22–1.36% of [18F]-fluorodeoxyglucose incorporation per million cells was found. Additionally in the protoporphyrine IX treated cells a significant 62% decrease of [18F]-fluorodeoxyglucose incorporation was observed (p < .05). A parallel significant decrease in alkaline phosphatase activity (p < .001) was found in the cells treated by the protoporphyrine IX. Therefore we suggest that the presented method of [18F]-fluorodeoxyglucose incorporation measurement can be utilized as an effective research tool for estimation of the cellular glycolitic activity in human osteoblast-like cells in vitro.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery “A”, Rambam Medical Center, P.O. 9602, Haifa, 31096, Israel

    Nahum Rosenberg & Michael Soudry

  2. Nuclear Medicine Department, Rambam Medical Center, Haifa, Israel

    Lise Bettman & Rachel Bar-Shalom

  3. Ruth and Bruce Rappaport Faculty of Medicine, Technion–Israel Institute of Technology, Haifa, Israel

    Nahum Rosenberg, Orit Rosenberg, Michael Soudry, Moshe Gavish & Rachel Bar-Shalom

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  1. Nahum Rosenberg
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  2. Lise Bettman
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  3. Orit Rosenberg
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  4. Michael Soudry
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  5. Moshe Gavish
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  6. Rachel Bar-Shalom
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Correspondence to Nahum Rosenberg.

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Rosenberg, N., Bettman, L., Rosenberg, O. et al. Measurement of [18F]-fluorodeoxyglucose incorporation into human osteoblast–An experimental method. Cytotechnology 54, 1–4 (2007). https://doi.org/10.1007/s10616-007-9066-3

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  • DOI: https://doi.org/10.1007/s10616-007-9066-3

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