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The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome

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Abstract

In recent years there have been a number of reports that suggest the sub-physiological (<37 °C) temperature in vitro culturing of mammalian cells can result in enhanced heterologous protein production. Despite these reports, the mechanisms by which mammalian cells respond to such conditions are largely unknown. We therefore set out to use a model in vitro culture HeLa cell system to begin investigating the cold-shock response in mammalian cell systems. Sub-physiological temperature cultivation resulted in reduced growth and proliferation and a lower total cell protein content. Proteomic analysis confirmed that HeLa cells actively respond to sub-physiological temperature by up-regulating a number of proteins and immunoblot analysis confirmed that specific proteins are indeed up-regulated in a time and temperature dependent manner. Additional work is likely to improve our understanding of the cold-shock response in mammalian cells and identify candidate target proteins for cell engineering to further enhance heterologous protein production at sub-physiological temperatures.

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Acknowledgements

This work was partially supported by Grant BB/C006569/1 from the Biotechnology and Biological Sciences Research Council, UK.

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Authors and Affiliations

  1. Protein Science Group, University of Kent, Canterbury, Kent, CT2 7NJ, UK

    Michèle F. Underhill & C. Mark Smales

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  1. Michèle F. Underhill
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  2. C. Mark Smales
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Correspondence to C. Mark Smales.

Additional information

A manuscript submitted from the Global mRNA and Protein Expression Analysis, Research Applications in Cancer and other Diseases and in Biopharmaceutical Production conference, Dublin City University, Dublin 9, Ireland, September 7–8th 2006

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Underhill, M.F., Smales, C.M. The cold-shock response in mammalian cells: investigating the HeLa cell cold-shock proteome. Cytotechnology 53, 47–53 (2007). https://doi.org/10.1007/s10616-007-9048-5

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  • DOI: https://doi.org/10.1007/s10616-007-9048-5

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