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Transcription of subtelomere tandemly repetitive DNA in chicken embryogenesis

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Abstract

Transcription of tandemly repetitive DNA in embryogenesis seems to be of special interest due to a crucial role of non-coding RNAs in many aspects of development. However, only a few data are available on tandem repeats transcription at subtelomere regions of chromosomes during vertebrate embryogenesis. To reduce this gap, we examined stage and tissue-specific pattern of subtelomeric PO41 (pattern of 41 bp) tandem repeat transcription during embryogenesis of chicken (Gallus gallus domesticus). Using whole-mount RNA fluorescent in situ hybridization and reverse transcription PCR with specific primers, we demonstrated that both strands of PO41 repeat are transcribed at each of the studied stages of chicken embryo development: from 7–8 HH to 20 HH stages. Subtelomere-derived transcripts localize in the nuclei of all cell types and throughout the all embryonic bodies: head, somites, tail, wings and buds. In embryo-dividing cells and cultured embryonic fibroblasts, PO41 RNAs envelop terminal regions of chromosomes. PO41-containing RNAs are predominantly single-stranded and can be polyadenylated, indicating appearance of non-nascent form of subtelomeric transcripts. PO41 repeat RNAs represent a rare example of ubiquitously transcribed non-coding RNAs, such as Xist/XIST RNA or telomere repeat-containing RNA. Distribution of PO41 repeat transcripts at different stages of embryo development and among cell types has extremely uniform pattern, indicating on possible universal functions of PO41 non-coding RNAs.

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Abbreviations

CEFs:

Chicken embryonic fibroblasts

HH:

Hamburger and Hamilton

DMEM:

Dulbecco’s modified Eagle’s medium

PO41:

Pattern of 41 bp

RNA FISH:

RNA fluorescent in situ hybridization

RT-PCR:

Reverse transcription polymerase chain reaction

WISH:

Whole-mount in situ hybridization

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Acknowledgments

We thank Antonina Maslova (St. Petersburg State University, Russia) for chicken embryonic fibroblasts collection and cultivation. This research was supported by Russian Science Foundation (grant #14-14-00131). The work was partially performed using experimental equipment of the Research Resource Centers ‘Chromas’ and ‘Molecular and cell technologies’ of St Petersburg State University.

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Authors and Affiliations

  1. Saint-Petersburg State University, 7-9, Universitetskaya nab., 199034, Saint-Petersburg, Russia

    Irina Trofimova, Darya Chervyakova & Alla Krasikova

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  1. Irina Trofimova
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  2. Darya Chervyakova
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  3. Alla Krasikova
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Correspondence to Alla Krasikova.

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Responsible Editors: Maria Assunta Biscotti, Pat Heslop-Harrison and Ettore Olmo

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Trofimova, I., Chervyakova, D. & Krasikova, A. Transcription of subtelomere tandemly repetitive DNA in chicken embryogenesis. Chromosome Res 23, 495–503 (2015). https://doi.org/10.1007/s10577-015-9487-3

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