Abstract
Using cross-species chromosome painting, we have carried out a comprehensive comparison of the karyotypes of two Ellobius species with unusual sex determination systems: the Transcaucasian mole vole, Ellobius lutescens (2n = 17, X in both sexes), and the northern mole vole, Ellobius talpinus (2n = 54, XX in both sexes). Both Ellobius species have highly rearranged karyotypes. The chromosomal paints from the field vole (Microtus agrestis) detected, in total, 34 and 32 homologous autosomal regions in E. lutescens and E. talpinus karyotypes, respectively. No difference in hybridization pattern of the X paint (as well as Y paint) probes on male and female chromosomes was discovered. The set of golden hamster (Mesocricetus auratus) chromosomal painting probes revealed 44 and 43 homologous autosomal regions in E. lutescens and E. talpinus karyotypes, respectively. A comparative chromosome map was established based on the results of cross-species chromosome painting and a hypothetical ancestral Ellobius karyotype was reconstructed. A considerable number of rearrangements were detected; 31 and 7 fusion/fission rearrangements differentiated the karyotypes of E. lutescens and E. talpinus from the ancestral Ellobius karyotype. It seems that inversions have played a minor role in the genome evolution of these Ellobius species.
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References
Borisov YM, Lyapunova EA, Vorontsov NN (1991) Karyotype evolution in the genus Ellobius (Microtinae, Rodentia). Genetika 27: 523–532 [in Russian].
Corbet GB (1978) The Mammals of the Palaearctic Region: A Taxonomic Review. London and Ithaca: British Museum (Natural History), Cornell University Press.
Corbet GB (1984) The Mammals of the Palaearctic Region: A Taxonomic Review. Supplement. London and Ithaca: British Museum (Natural History), Cornell University Press.
Fredga K, Lyapunova EA (1991) Fertile males with two X chromosomes in Ellobius tancrei (Rodentia, Mammalia). Hereditas 11: 86–97.
Graphodatsky AS, Yang F, O’Brien PC et al. (2000) A comparative chromosome map of the Arctic fox, red fox and dog defined by chromosome painting and high resolution G-banding. Chromosome Res 8: 253–263.
Just W, Rau W, Vogel W et al. (1995) Absence of Sry in species of the vole Ellobius. Nature Genet 11: 117–118.
Just W, Baumstark A, Hameister H et al. (2002) The sex determination in Ellobius lutescens remains bizarre. Cytogenet Genome Res 96: 146–153.
Li T, Wang J, Su W, Yang F (2006) Chromosomal mechanisms underlying the karyotype evolution of the oriental voles (Muridae, Eothenomys). Cytogenet Genome Res 114: 50–55.
Lyapunova EA, Vorontsov NN, Korobitsyna et al. (1980) A robertsonian fan in Ellobius talpinus. Genetica 53/54: 239–247.
Lyapunova EA, Baklushinskaya IY, Kolomiets OL, Mazurova TF (1990) Analysis of fertility of hybrids of multi-chromosomal forms in mole-voles of the super-species Ellobius tancrei differing in a single pair of Robertsonian metacentrics. Doklady Akademii Nauk SSSR 310: 721–723 [in Russian].
Matthey R (1953) La formule chromosomique et le probleme de la determination sexuelle chez Ellobius lutescens Thomas (Rodentia-Muridae-Microtinae). Arch Klaus-Stift Vererb Forsch 28: 65–73.
Musser GG, Carleton MD (2005) Subfamily Arvicolinae. In: Wilson DE, Reeder DM, eds. Mammal Species of the World: A Taxonomic and Geographic Reference. Baltimore: Johns Hopkins University Press, pp 956–1039.
Pavlinov IY, Rossolimo OL (1987) Systematics of the Mammals of the USSR. Moscow: Moscow University Press [in Russian].
Perelman PL, Graphodatsky AS, Baklushinskaya IY, Lyapunova EA (2006) Selected karyotypes. In: O'Brien SJ, Nash WG, Menninger JC, eds. ATLAS of Mammalian Karyotypes. John Wiley and Sons Publishers, p 279.
Romanenko SA, Perelman PL, Serdukova NA et al. (2006) Reciprocal chromosome painting between three laboratory rodent species. Mamm Genome 17: 1183–1192.
Seabright M (1971) A rapid banding technique for human chromosomes. Lancet 2: 971–972.
Sitnikova NA, Romanenko SA, O’Brien et al. (2007) Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). I. The genome homology of tundra vole, field vole, mouse and golden hamster revealed by comparative chromosome painting. Chromosome Res 15: 447–456.
Stanyon R, Yang F, Morescalchi AM, Galleni L (2004) Chromosome painting in the long-tailed field mouse provides insights into the ancestral murid karyotype. Cytogenet Genome Res 105: 406–411.
Vogel W, Jainta S, Rau W et al. (1998) Sex determination in Ellobius lutescens: the story of an enigma. Cytogenet Cell Genet 80: 214–221.
Vorontsov NN, Radjably SI (1967) Chromosomal complements and cytogenetical differentiation of two forms of Ellobius talpinus Pall. subspecies. Tsitologya 9: 848–852 [in Russian].
Vorontsov NN, Yakimenko LV (1984) The morphometric analysis of the skull in mole vole (Rodentia, Ellobius). Zool J 63: 1859–1871 [in Russian].
Yang F,O’Brien PC, Milne BS et al. (1999) A complete comparative chromosome map for the dog, red fox, and human and its integration with canine genetic maps. Genomics 62: 189–202.
Yang E, O’Brien PCM, Ferguson-Smith MA (2000) Comparative chromosome map of the laboratory mouse and Chinese hamster defined by reciprocal chromosome painting. Chromosome Res 8: 219–227.
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Romanenko, S.A., Sitnikova, N.A., Serdukova, N.A. et al. Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). II. The genome homology of two mole voles (genus Ellobius), the field vole and golden hamster revealed by comparative chromosome painting. Chromosome Res 15, 891–897 (2007). https://doi.org/10.1007/s10577-007-1171-9
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DOI: https://doi.org/10.1007/s10577-007-1171-9