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Changes in gene expression during male meiosis in Petunia hybrida

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Abstract

We analyzed changes in gene expression during male meiosis in Petunia by combining the meiotic staging of pollen mother cells from a single anther with cDNA-AFLP transcript profiling of mRNA from the synchronously developing sister anthers. The transcript profiling experiments focused on the identification of genes with a modulated expression profile during meiosis, while premeiotic archesporial cells and postmeiotic microspores served as a reference. About 8000 transcript tags, estimated at 30% of the total transcriptome, were generated, of which around 6% exhibited a modulated gene expression pattern at meiosis. Cluster analysis revealed a transcriptional cascade that coincides with the initiation and progression through all stages of the two meiotic divisions. Fragments that exhibited high expression specifically during meiosis I were characterized further by sequencing; 90 out of the 293 sequenced fragments showed homology with known genes, belonging to a wide range of gene classes, including previously characterized meiotic genes. In-situ hybridization experiments were performed to determine the spatial expression pattern for five selected transcript tags. Its concurrence with cDNA-AFLP transcript profiles indicates that this is an excellent approach to study genes involved in specialized processes such as meiosis. Our data set provides the potential to unravel unique meiotic genes that are as yet elusive to reverse genetics approaches.

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Authors and Affiliations

  1. Institute for Wetland and Water Research, Department of Experimental Botany, Section Plant Genetics, Radboud University, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands

    Filip Cnudde, Veena Hedatale, Koen Weterings, Tom Gerats & Janny L. Peters

  2. Laboratory of Genetics, Wageningen University, Arboretumlaan 4, 6703 HA, Wageningen, The Netherlands

    Hans de Jong

  3. Department of General Instrumentation, Radboud University, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands

    Elisabeth S. Pierson

  4. Keygene N.V., P.O. Box 216, 6700AE, Wageningen, The Netherlands

    Daphne Y. Rainey & Tom Gerats

  5. Department of Plant Systems Biology, VIB/Ghent University, Technologiepark 927, B-9052, Ghent, Belgium

    Marc Zabeau

Authors
  1. Filip Cnudde
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  2. Veena Hedatale
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  4. Elisabeth S. Pierson
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  7. Koen Weterings
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  8. Tom Gerats
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  9. Janny L. Peters
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Corresponding author

Correspondence to Janny L. Peters.

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*Filip Cnudde and Veena Hedatale have equally contributed to this work.

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Transcript Profiling Experiment Male Meiosis in Petunia Anthers (doc 130,560 kb)

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Cnudde, F., Hedatale, V., de Jong, H. et al. Changes in gene expression during male meiosis in Petunia hybrida . Chromosome Res 14, 919–932 (2006). https://doi.org/10.1007/s10577-006-1099-5

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  • DOI: https://doi.org/10.1007/s10577-006-1099-5

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