Abstract
Chromodomain helicase/ATPase DNA binding protein 1-like (CHD1L) gene is a newly identified oncogene located at Chr1q21 and it is amplified in many solid tumors. In this study, we intended to investigate the clinical significance of CHD1L expression in human glioma and its biological function in glioma cells. Western blot and immunohistochemistry analysis showed that CHD1L was overexpressed in glioma tissues and glioma cell lines. In addition, the expression level of CHD1L was positively correlated with glioma pathological grade and Ki-67 expression. Kaplan–Meier curve indicated that high expression of CHD1L may result in poor prognosis of glioma patients. Accordingly, suppression of CHD1L in glioma cells was shown to induce cell cycle arrest and increase apoptosis. In addition, knockdown of CHD1L significantly accelerated migration and invasion ability of glioma cells. Together our findings suggest that CHD1L is involved in the progression of glioma and may be a novel target for further therapy.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (Nos. 81201858, 81272789), the National Natural Science Foundation of Jiangsu province (No. BK2012231), and the Nantong Society Undertaking and Technological Innovation (HS2014069).
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Jie Sun and Li Zhang have contributed equally to this work.
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Sun, J., Zhang, L., Zhao, H. et al. CHD1L Regulates Cell Cycle, Apoptosis, and Migration in Glioma. Cell Mol Neurobiol 36, 565–576 (2016). https://doi.org/10.1007/s10571-015-0237-z
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DOI: https://doi.org/10.1007/s10571-015-0237-z