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CDC25A mRNA levels significantly correlate with Ki-67 expression in human glioma samples

  • Laboratory Investigation - Human/Animal Tissue
  • Published:
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Abstract

Cell division cycle 25 (CDC25) phosphatases are cell-cycle regulatory proteins which are overexpressed in a significant number of human cancers. This study evaluated the role of CDC25 phosphatases in human glioma proliferation. Upregulation of CDC25A was observed in human glioma specimens and human glioma cell lines. Comparison of expression levels of CDC25A and CDC25B messenger ribonucleic acid (RNA) to Ki-67 labeling index in glioma tissues found that Ki-67 labeling index was significantly correlated with the expression of CDC25A, but not with that of CDC25B. Depletion of CDC25A by small interfering RNA and inhibition of CDC25 suppressed cell proliferation and induced apoptosis in glioma cell lines, indicating that CDC25A is a potential target for the development of new therapy for glioma.

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Acknowledgments

This work was supported in part by Grants-in-aid for Scientific Research (C) to Y.Y. and H.S. and for Exploratory Research to R.K. provided by the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Division of Cancer Chemotherapy, Miyagi Cancer Center Research Institute, 47-1 Nodayama, Medeshima-Shiode, Natori, Miyagi, 981-1293, Japan

    Yoji Yamashita, Isao Kasugai, Masami Sato, Nobuhiro Tanuma, Miyuki Nomura & Hiroshi Shima

  2. Department of Neurosurgery, Miyagi Cancer Center, Natori, Miyagi, Japan

    Yoji Yamashita & Ryuichi Katakura

  3. Section of Clinical Research, Miyagi Cancer Center Research Institute, Natori, Miyagi, Japan

    Ikuro Sato

  4. Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa, Japan

    Katsumi Yamashita

  5. Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan

    Yukihiko Sonoda, Toshihiro Kumabe & Teiji Tominaga

Authors
  1. Yoji Yamashita
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  2. Isao Kasugai
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  3. Masami Sato
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  4. Nobuhiro Tanuma
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  5. Ikuro Sato
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  6. Miyuki Nomura
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  7. Katsumi Yamashita
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  8. Yukihiko Sonoda
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  9. Toshihiro Kumabe
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  10. Teiji Tominaga
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  11. Ryuichi Katakura
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  12. Hiroshi Shima
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Corresponding author

Correspondence to Hiroshi Shima.

Additional information

Yoji Yamashita and Isao Kasugai contributed equally to this work.

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Yamashita, Y., Kasugai, I., Sato, M. et al. CDC25A mRNA levels significantly correlate with Ki-67 expression in human glioma samples. J Neurooncol 100, 43–49 (2010). https://doi.org/10.1007/s11060-010-0147-3

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  • DOI: https://doi.org/10.1007/s11060-010-0147-3

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