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Dynamic Expressions of Beclin 1 and Tyrosine Hydroxylase in Different Areas of 6-Hydroxydopamine-Induced Parkinsonian Rats

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Abstract

Beclin 1, a regulator of the autophagy pathway, plays an important role in Parkinson’s disease (PD). However, the crucial mechanism of Beclin 1 in PD remains unclear. Therefore, we investigated dynamic expressions of Beclin 1 and tyrosine hydroxylase (TH) in different brain areas of 6-OHDA-induced rats. Beclin 1 and TH expressions were analyzed by flow cytometry and immunohistochemistry, respectively. The results showed that Beclin 1 expressions were low in the sham group, but rose significantly after 6-OHDA injection. In the striatum and cortex, Beclin 1 increased at 3 h, peaking at 12 h, while in the hippocampus, it increased at 3 h and peaked at 24 h, then it declined slowly and remained steady at 72 h. Beclin 1 expression in the striatum and cortex areas was higher than that of the hippocampus area at 12 h. In addition, the time-course of TH expression in the striatum was similar to that in the mesencephalon. TH expression declined dramatically between 0 and 12 h. Pearson analysis showed significant negative correlations between TH and Beclin 1 expression in the areas we analyzed. While TH expression declined gradually between 12 and 72 h, significant positive correlations between TH and Beclin 1 were detected during that interval. This indicated that activation of Beclin 1-dependent autophagy may inhibit the loss of TH-positive neurons.

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Acknowledgments

This work was supported by the Guangdong Natural Science Foundation of China (No. S2012010010625). We would like to express our sincere thanks to the reviewers and editors for the constructive and positive comments.

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The authors do not have any conflict of interest.

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Authors and Affiliations

  1. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

    Sheng Zhang, Zhong-Feng Xue, Li-Ping Huang, Ruo-Ming Fang, Yu-Ping He, Ling Li & Yong-Qi Fang

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  1. Sheng Zhang
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  2. Zhong-Feng Xue
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  3. Li-Ping Huang
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  4. Ruo-Ming Fang
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  5. Yu-Ping He
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  6. Ling Li
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  7. Yong-Qi Fang
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Correspondence to Yong-Qi Fang.

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Zhang, S., Xue, ZF., Huang, LP. et al. Dynamic Expressions of Beclin 1 and Tyrosine Hydroxylase in Different Areas of 6-Hydroxydopamine-Induced Parkinsonian Rats. Cell Mol Neurobiol 33, 973–981 (2013). https://doi.org/10.1007/s10571-013-9964-1

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