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Protective Effect of Paeoniflorin on Aβ25–35-Induced SH-SY5Y Cell Injury by Preventing Mitochondrial Dysfunction

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Abstract

Alzheimer’s disease (AD) is a major neurodegenerative brain disorder affecting about 14 million people worldwide. Aβ-induced cell injury is a crucial cause of neuronal loss in AD, thus the suppression of which might be useful for the treatment of this disease. In this study, we aimed to evaluate the effect of paeoniflorin (PF), a monoterpene glycoside isolated from aqueous extract of Radix Paeoniae Alba, on Aβ25–35-induced cytotoxicity in SH-SY5Y cells. The results showed PF could attenuate or restore the viability loss, apoptotic increase, and ROS production induced by Aβ25–35 in SH-SY5Y cells. In addition, PF strikingly inhibited Aβ25–35-induced mitochondrial dysfunction, which includes decreased mitochondrial membrane potential, increased Bax/Bcl-2 ratio, cytochrome c release and activity of caspase-3 and caspase-9. Therefore, our study provided the first experimental evidence that PF could modulate ROS production and apoptotic mitochondrial pathway in model of neuron injury in vitro and which might provide new insights into its application toward Alzheimer’s disease therapy.

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Acknowledgments

This work is supported by Grants from National Natural Science Foundation (81300787) and the Natural Science Foundation of Jiangsu Province (BK2011168, BK2012105).

Conflict of interest

Ke Wang and other co-authors have no conflict of interest

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Authors and Affiliations

  1. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu Province, China

    Ke Wang, Xue Zhu, Kai Zhang, Biao Huang, Jue Zhang, Yi Zhang, Lan Zhu & Bin Zhou

  2. Save Sight Institute, University of Sydney, Sydney, NSW, 2000, Australia

    Ling Zhu

  3. Faculty of Pharmacy, University of Sydney, Sydney, NSW, 2006, Australia

    Fanfan Zhou

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  1. Ke Wang
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  2. Ling Zhu
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  3. Xue Zhu
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  4. Kai Zhang
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  6. Jue Zhang
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  7. Yi Zhang
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Correspondence to Ke Wang.

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Wang, K., Zhu, L., Zhu, X. et al. Protective Effect of Paeoniflorin on Aβ25–35-Induced SH-SY5Y Cell Injury by Preventing Mitochondrial Dysfunction. Cell Mol Neurobiol 34, 227–234 (2014). https://doi.org/10.1007/s10571-013-0006-9

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  • DOI: https://doi.org/10.1007/s10571-013-0006-9

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