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Different Temporal Patterns in the Expressions of Bone Morphogenetic Proteins and Noggin During Astroglial Scar Formation After Ischemic Stroke

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Abstract

Bone morphogenetic proteins (BMPs) and their antagonists have roles in scar formation and regeneration after central nervous system injuries. However, temporal changes in their expression during astroglial scar formation in the ischemic brain are unknown. Here, we examined protein levels of BMP2, BMP7, and their antagonist noggin in the ischemic brain up to 4 weeks after experimental stroke in mice. BMP2 and BMP7 levels were increased from 1 to 4 weeks in the ischemic brain, and their expression was associated with astrogliosis. BMP7 expression was more intense and co-localized in reactive astrocytes in the ischemic subcortex at 1 week. Noggin expression began to increase after 2 weeks and was further increased at 4 weeks only in the ischemic subcortex, but the intensity was weak compared to the intensity of BMPs. Noggin was co-localized mainly in activated microglia. These findings show that expression of BMPs and noggin differed over time, in intensity and in types of cell, and suggest that BMPs and noggin have different roles in the processes of glial scar formation and neurorestoration in the ischemic brain.

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Abbreviations

BMPs:

Bone morphogenetic proteins

CBF:

Cerebral blood flow

GFAP:

Glial fibrillary acidic protein

Iba1:

Ionized calcium binding adaptor molecule 1

MCAO:

Middle cerebral artery occlusion

OPC:

Oligodendrocyte precursor cell

PLSD:

Protected least significant difference

TBS:

Tris-buffered saline

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Acknowledgments

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0011353 to E.M.P. and 2010-0029353 to J.L.K.).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmacology, Ewha Medical Research Institute, School of Medicine, Ewha Womans University, 911-1 Mok6dong Yangcheon-gu, Seoul, 158-710, Republic of Korea

    Jin A. Shin, Kyung-Eun Lee & Eun-Mi Park

  2. Department of Physiology, Tissue Injury Defense Research Center, Ewha Medical Research Institute, School of Medicine, Ewha Womans University, 911-1 Mok6dong Yangcheon-gu, Seoul, 158-710, Republic of Korea

    Jihee Lee Kang

  3. Department of Brain & Cognitive Sciences, Brain Disease Research Institute, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul, 120-750, Republic of Korea

    Eun-Mi Park

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  1. Jin A. Shin
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Correspondence to Eun-Mi Park.

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Shin, J.A., Kang, J.L., Lee, KE. et al. Different Temporal Patterns in the Expressions of Bone Morphogenetic Proteins and Noggin During Astroglial Scar Formation After Ischemic Stroke. Cell Mol Neurobiol 32, 587–597 (2012). https://doi.org/10.1007/s10571-012-9806-6

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