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Postnatal Development of Neurons, Interneurons and Glial Cells in the Substantia Nigra of Mice

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Abstract

We investigated postnatal alterations of neurons, interneurons and glial cells in the mouse substantia nigra using immunohistochemistry. Tyrosine hydroxylase (TH), neuronal nuclei (NeuN), parvalbumin (PV), neuronal nitric oxide synthase (nNOS), glial fibrillary acidic protein (GFAP), ionized calcium-binding adaptor molecule 1 (Iba 1), CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase), brain-derived neurotrophic factor (BDNF) and glial cell-line-derived neurotrophic factor (GDNF) immunoreactivity were measured in 1-, 2-, 4- and 8-week-old mice. In the present study, the maturation of NeuN-immunopositive neurons preceded the production of TH in the substantia nigra during postnatal development in mice. Furthermore, the maturation of nNOS-immunopositive interneurons preceded the maturation of PV-immunopositive interneurons in the substantia nigra during postnatal development. Among astrocytes, microglia and oligodendrocytes, in contrast, the development process of oligodendrocytes is delayed in the substantia nigra. Our double-labeled immunohistochemical study suggests that the neurotrophic factors such as BDNF and GDNF secreted by GFAP-positive astrocytes may play some role in maturation of neurons, interneurons and glial cells of the substantia nigra during postnatal development in mice. Thus, our findings provide valuable information on the development processes of the substantia nigra.

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Authors and Affiliations

  1. Department of Neurobiology and Therapeutics, Graduate School and Faculty of Pharmaceutical Sciences, The University of Tokushima, 1-78, Sho-machi, Tokushima, 770-8505, Japan

    Manami Abe, Hiroki Kimoto, Risa Eto, Taeko Sasaki, Jiro Kasahara & Tsutomu Araki

  2. Department of Neurology, Organized Center of Clinical Medicine, International University of Health and Welfare Hospital, Tochigi, Japan

    Hiroyuki Kato

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  1. Manami Abe
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  2. Hiroki Kimoto
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  3. Risa Eto
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  4. Taeko Sasaki
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  5. Hiroyuki Kato
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  6. Jiro Kasahara
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  7. Tsutomu Araki
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Correspondence to Tsutomu Araki.

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Abe, M., Kimoto, H., Eto, R. et al. Postnatal Development of Neurons, Interneurons and Glial Cells in the Substantia Nigra of Mice. Cell Mol Neurobiol 30, 917–928 (2010). https://doi.org/10.1007/s10571-010-9521-0

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  • DOI: https://doi.org/10.1007/s10571-010-9521-0

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