Abstract
Leucine-rich glioma inactivated (LGI) 3 encodes a leucine-rich repeat protein. The precise function of LGI3, however, remains unknown. We have previously shown that amyloid-β peptide (Aβ) upregulates LGI3 and that Aβ and LGI3 colocalize on plasma membranes of cultured rat astrocytes. In the present study, we performed immunohistochemical and biochemical analyses of LGI3 using various aged monkey brains. Immunohistochemistry showed that LGI3 was present in almost all neural cells and mainly localized at plasma membranes and nuclei. In aged monkey brains, we found that LGI3 accumulated on or near the plasma membranes of neurons, and colocalized with endocytosis-associated proteins and lipid raft markers. Double immunohistochemistry also showed that LGI3 colocalized with Aβ in astrocytes of aged brains. Moreover, Western blot analyses revealed that LGI3 may be cleaved in brain. Additionally, in aged monkeys LGI3 accumulated in microsomal and nuclear brain fractions.
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Acknowledgment
This study was supported by a grant-in-aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (18790288).
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Okabayashi, S., Kimura, N. Immunohistochemical and Biochemical Analyses of LGI3 in Monkey Brain: LGI3 Accumulates in Aged Monkey Brains. Cell Mol Neurobiol 27, 819–830 (2007). https://doi.org/10.1007/s10571-007-9205-6
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DOI: https://doi.org/10.1007/s10571-007-9205-6