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Aβ Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes

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1. The leucine-rich glioma inactivated (LGI) family of genes encodes a leucine-rich repeat (LRR) protein, proteins that are thought to be specifically involved in protein–protein and protein–matrix interactions. Since amyloid beta peptide (Aβ) has been previously shown to induce the expression of another LRR-encoding gene in neural cells, we assessed how Aβ affects LGI gene expression in rat primary cerebral cortical cultures and astrocyte cultures. Both RT-PCR and Western Blotting analyses revealed that Aβ robustly induced the expression of LGI3 in rat astrocyte cultures.

2. Western Blotting analyses also showed that both glial fibrillary acidic protein (GFAP) and apolipoprotein E (ApoE) significantly increased coincidentally with the Aβ-induced upregulation of LGI3. Immunocytochemistry showed that LGI3 colocalized with Aβ at plasma membranes and also with internalized Aβ in astrocytes. These findings suggest that activated LGI3 may be involved in the astroglial response against Aβ.

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ACKNOWLEDGMENTS

This study was supported by a grant-in-aid from Research on Human Genome, Tissue Engineering, Ministry of Health, Labor, and Welfare, Japan. The authors are especially thankful to the MBL Company for producing the LGI3-specific antibody anti-TA142.

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Authors and Affiliations

  1. Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Ibaraki, Japan

    Nobuyuki Kimura

  2. Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Nobuyuki Kimura, Yoshiyuki Ishii, Shingo Suzaki, Takayuki Negishi, Shigeru Kyuwa & Yasuhiro Yoshikawa

  3. Department of Chemistry and Biological Science, School of Science and Engineering, Aoyama Gakuin University, Kanagawa, Japan

    Takayuki Negishi

  4. Laboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, Tsukuba-shi, Ibaraki, 35-0843, Japan

    Nobuyuki Kimura

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  1. Nobuyuki Kimura
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Correspondence to Nobuyuki Kimura.

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Kimura, N., Ishii, Y., Suzaki, S. et al. Aβ Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes. Cell Mol Neurobiol 27, 335–350 (2007). https://doi.org/10.1007/s10571-006-9127-8

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  • DOI: https://doi.org/10.1007/s10571-006-9127-8

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