1. The present study aimed at elucidating the effect of nitric oxide (NO) on blood-brain barrier (BBB) function with mouse brain capillary endothelial (MBEC4) cells.
2. Histamine (20–100 μM) evoked NO production (1.6–7 μM) in MBEC4 cells in a dose-dependent manner.
3. The permeability coefficient of sodium fluorescein for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were increased dose-dependently by the addition of NO solutions (14 and 28 μM) every 10 min during a 30-min period.
4. The present study demonstrated that NO increased the permeability and inhibited the P-glycoprotein efflux pump of brain capillary endothelial cells, suggesting that NO plays an inhibitory role in the dynamic regulation of the BBB function.
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ACKNOWLEDGMENTS
This work was supported in part by Grants-in-Aid for Scientific Research ((B)(2) 14370789) and ((C)(2) 15590475) from JSPS, Japan, by a Grant-in-Aid for Exploratory Research (16659138) from MEXT, Japan, and by funds (No.: 031001) from the Central Research Institute of Fukuoka University.
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Yamauchi, A., Dohgu, S., Nishioku, T. et al. An Inhibitory Role of Nitric Oxide in the Dynamic Regulation of the Blood-Brain Barrier Function. Cell Mol Neurobiol 27, 263–270 (2007). https://doi.org/10.1007/s10571-007-9139-z
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DOI: https://doi.org/10.1007/s10571-007-9139-z