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Microarray Analysis of Gene Expression in the Supraoptic Nucleus of Normoosmotic and Hypoosmotic Rats

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1. Hypoosmolality produces a dramatic inhibition of vasopressin (VP) and oxytocin (OT) gene expression in the supraoptic nucleus (SON). This study examines the effect of sustained hypoosmolality on global gene expression in the OT and VP magnocellular neurons (MCNs) of the hypothalamo-neurohypophysial system (HNS), in order to detect novel genes in this system that might be involved in osmoregulation in the MCNs.

2. For this purpose, we used Affymetrix oligonucleotide arrays to analyze the expression of specific genes in laser microdissected rat SONs, and their changes in expression during chronic hypoosmolality. We identified over 40 genes that had three-fold or more greater expression in the SON versus total hypothalamus, and that also changed more than two fold in expression as a result of the chronic hypoosmolar treatment. These genes contained both novel as well as genes previously known to be present in the SON. All of the raw data for the genes that are expressed in the SON and altered by hypoosmolality can be found on the following NINDS website URL address: http://data.ninds.nih.gov/Gainer/Publications

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Abbreviations

MCN:

magnocellular neuron

SON:

supraoptic nucleus

HNS:

hypothalamoneurohypophysial

LMD:

laser microdissection

OT:

oxytocin

VP:

vasopressin

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ACKNOWLEDGEMENTS

We thank Dr. Ying Tian (Georgetown University) for her help with the preparation of the hypoosmotic animals, Mr. Raymond L. Fields (NINDS, NIH) for his molecular biological advice and assistance, Mr. James W. Nagle and Ms. Debbie Kauffman (NINDS, DNA sequencing Facility) for DNA sequencing, Dr. Abdel Elkahloun (Hybridization Facility, NHGRI) for his advice and technical support with Affymetrix hybridization procedures, Dr. Babru Samal (NIMH) for his advice about Bioinformatics, and Dr. Catherine Campbell (NINDS, NIH) for her critical advice and assistance with the microarray analysis, as well as for discussions about Bioinformatics. This research was supported by the Intramural research program of the NIH, NINDS.

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Authors and Affiliations

  1. Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, USA

    Chunmei Yue, Noriko Mutsuga & Harold Gainer

  2. Department of Medicine, Division of Endocrinology and Metabolism, Georgetown University, Washington, DC, 20007, USA

    Joseph Verbalis

  3. Molecular Neuroscience Section, Laboratory of Neurochemistry, National Institute of Neurological Disorder and Stroke, National Institutes of Health, Bldg. 49, Rm 5A78, Bethesda, MD, 20892, USA

    Harold Gainer

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  1. Chunmei Yue
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  2. Noriko Mutsuga
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  3. Joseph Verbalis
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  4. Harold Gainer
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Correspondence to Harold Gainer.

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Yue, C., Mutsuga, N., Verbalis, J. et al. Microarray Analysis of Gene Expression in the Supraoptic Nucleus of Normoosmotic and Hypoosmotic Rats. Cell Mol Neurobiol 26, 957–976 (2006). https://doi.org/10.1007/s10571-006-9017-0

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