Abstract
1. We have investigated the effect of tityus gamma (TiTX γ) scorpion toxin on the release of [3H] dopamine in rat brain prefrontal cortical slices. The stimulatory effect of TiTX γ on the release of [3H]dopamine was dose/time-dependent with an EC50 of 0.01μM.
2. Tetrodotoxin blocked the TiTX γ-induced release of [3H]dopamine, indicating the dependency for Na+ channels.
3. EGTA had no effect on the TiTX γ-induced release of [3H]dopamine, indicating the process is independent of extracellular calcium. Release of [3H]dopamine evoked by TiTX γ was inhibited by 57% by BAPTA, a chelator of intracellular calcium.
4. Xestospongin and 2-APB, putative blockers of IP3-sensitive release of intracellular calcium stores, caused an equal and significant inhibition of 24% of the TiTX γ-induced release of [3H]dopamine, while the slight inhibition evoked by dantrolene, a putative blocker of ryanodine-sensitive calcium store was not significant.
5. Nomifensine and ascorbic acid, blockers of dopamine transporter (DAT), caused an inhibition of 27 and 29%, respectively, on the toxin-induced release of [3H]dopamine suggesting that most of the TiTX γ-induced release of dopamine is not due to the reversal of Na+ gradient.
6. In conclusion the majority of the TiTX γ-induced release of [3H]dopamine is exocytotic and mobilizes calcium from the intracellular IP3-sensitive calcium stores.
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Fernandes, V.M.V., Romano-Silva, M.A., Gomes, D.A. et al. Dopamine Release Evoked by Beta Scorpion Toxin, Tityus Gamma, in Prefrontal Cortical Slices is Mediated by Intracellular Calcium Stores. Cell Mol Neurobiol 24, 757–767 (2004). https://doi.org/10.1007/s10571-004-6917-8
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DOI: https://doi.org/10.1007/s10571-004-6917-8