Abstract
Effect of cationic surfactants alkyltrimethylammonium bromide (CnTAB) with varied alkyl chain lengths on the enzymatic hydrolysis of Avicel and the surface charge of cellulase was investigated. Enzymatic hydrolysis of Avicel increased linearly from 42.1 to 61.4 % with the increase of the concentration of cetyltrimethylammonium bromide (C16TAB) logarithmically from 0.0001 to 0.01 mM, and reached a maximum value at the concentration of 0.01–0.03 mM. When the concentration was increased further, the cellulase solution became positively charged and the enzymatic hydrolysis of Avicel decreased rapidly. With the increasing alkyl chain length, CnTAB provided more proton and neutralized the negative charge of cellulase more obviously. Therefore, the required concentration of CnTAB could be less to enhance the enzymatic hydrolysis of Avicel. In addition, C16TAB could enhance enzymatic hydrolysis efficiency of corncob at high solid content from 35.0 to 56.3 %; C16TAB could reduce about 60 % of the cellulase loading in the enzymatic hydrolysis of corncob to obtain the same glucose yield. Effect of C16TAB on the enzymatic hydrolysis of typical pretreated softwood and hardwood was also investigated. This study laid the foundation for using CnTAB to recover cellulase, and provided the design direction for cellulase with higher activity and better stability by adjusting its hydrophilicity and chargeability.
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Abbreviations
- CnTAB:
-
Alkyltrimethylammonium bromide
- C10TAB:
-
Decyltrimethylammonium bromide
- C12TAB:
-
Dodecyltrimethylammonium bromide
- C14TAB:
-
Tetradecyltrimethylammonium bromide
- C16TAB:
-
Cetyltrimethylammonium bromide
- MP:
-
Masson pine
- EW:
-
Eucalyptus wood
- DA:
-
Dilute acid pretreatment
- SPORL:
-
Sulfite pretreatment to overcome the recalcitrance of lignocelluloses
- CTec2:
-
Commercial cellulase enzyme Cellic CTec2
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Acknowledgments
The authors acknowledge the financial support of the National Natural Science Foundation of China (21376100, 21676109), and Guangdong Province Science and Technology Plan (2013B051000011).
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Lin, X., Cai, C., Lou, H. et al. Effect of cationic surfactant cetyltrimethylammonium bromide on the enzymatic hydrolysis of cellulose. Cellulose 24, 61–68 (2017). https://doi.org/10.1007/s10570-016-1089-5
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DOI: https://doi.org/10.1007/s10570-016-1089-5