Skip to main content

Advertisement

SpringerLink
Log in

Facilely green synthesis of silver nanoparticles into bacterial cellulose

  • Original Paper
  • Published:
Cellulose Aims and scope Submit manuscript

Abstract

A facile “Green Synthesis” method was used to prepare silver nanoparticles (AgNPs) in bacterial cellulose (BC) nanofibers, using BC as both the reducing and protecting agents with different concentration of AgNO3 (from 0.001 to 1 M) at 0.103 MPa pressure, 121 °C for 10 min without any other chemicals. The structures and properties of AgNPs/BC were characterized by UV–Visible spectral analysis, X-ray diffraction, field emission scanning electron microscopy, high-resolution transmission electron microscope, and inductively coupled plasma (ICP). The results showed that the AgNPs were spherical or nearly spherical with the face-centred cubic geometry and distributed uniformly in the three-dimensional network structure of BC with the size of about 40–60 nm. The silver content and release of silver ions were determined by ICP. With the increase of AgNO3 concentration, the content of silver increased from 16 to 115 mg/100 cm2 and the release of silver ions can be controlled by using different concentration of AgNO3. The antimicrobial activities of AgNPs/BC membranes against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were estimated by the AATCC Test Method 100-2004. According to these results, it is anticipated that the AgNPs/BC nanocomposites can be applied to the antimicrobial wound dressings and implant materials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahamed M, AlSalhi MS, Siddiqui MKJ (2010) Silver nanoparticle applications and human health. Clin Chim Acta 411(23–24):1841–1848

    Article  CAS  Google Scholar 

  • Alt V, Bechert T, Steinrucke P, Wagener M, Seidel P, Dingeldein E, Domann E, Schnettler R (2004) An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials 25(18):4383–4391

    Article  CAS  Google Scholar 

  • Bodin A, Ahrenstedt L, Fink H, Brumer H, Risberg B, Gatenholm P (2007) Modification of nanocellulose with a xyloglucan-RGD conjugate enhances adhesion and proliferation of endothelial cells: implications for tissue engineering. Biomacromolecules 8(12):3697–3704

    Article  CAS  Google Scholar 

  • Bodin A, Bharadwaj S, Wu S, Gatenholm P, Atala A, Zhang Y (2010) Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion. Biomaterials 31(34):8889–8901

    Article  CAS  Google Scholar 

  • Cai Z, Kim J (2010) Bacterial cellulose/poly(ethylene glycol) composite: characterization and first evaluation of biocompatibility. Cellulose 17(1):83–91

    Article  CAS  Google Scholar 

  • Cai J, Kimura S, Wada M, Kuga S (2009) Nanoporous cellulose as metal nanoparticles support. Biomacromolecules 10(1):87–94

    Article  CAS  Google Scholar 

  • Carlson C, Hussein SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, Schlager JJ (2008) Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B 112(43):13608–13619

    Article  CAS  Google Scholar 

  • Czaja W, Krystynowicz A, Bielecki S, Brown RM Jr (2006) Microbial cellulose—the natural power to heal wounds. Biomaterials 27(2):145–151

    Article  CAS  Google Scholar 

  • Czaja WK, Young DJ, Kawecki M, Brown RM (2007) The future prospects of microbial cellulose in biomedical applications. Biomacromolecules 8(1):1–12

    Article  CAS  Google Scholar 

  • de Santa Maria LC, Santos ALC, Oliveira PC, Barud HS, Messaddeq Y, Ribeiro SJL (2009) Synthesis and characterization of silver nanoparticles impregnated into bacterial cellulose. Mater Lett 63(9–10):797–799

    Article  Google Scholar 

  • Eichhorn SJ, Dufresne A, Aranguren M, Marcovich NE, Capadona JR, Rowan SJ, Weder C, Thielemans W, Roman M, Renneckar S, Gindl W, Veigel S, Keckes J, Yano H, Abe K, Nogi M, Nakagaito AN, Mangalam A, Simonsen J, Benight AS, Bismarck A, Berglund LA, Peijs T (2010) Review: current international research into cellulose nanofibres and nanocomposites. J Mater Sci 45(1):1–33

    Article  CAS  Google Scholar 

  • Frankel VH, Serafica GC, Damien CJ (2004) Development and testing of a novel biosynthesized XCell for treating chronic wounds. Surg Technol Int 12:27–33

    Google Scholar 

  • Gorth DJ, Rand DM, Webster TJ (2011) Silver nanoparticle toxicity in Drosophila: size does matter. Int J Nanomed 6:343–350

    CAS  Google Scholar 

  • He J, Kunitake T, Nakao A (2003) Facile in situ synthesis of noble metal nanoparticles in porous cellulose fibers. Chem Mater 15(23):4401–4406

    Article  CAS  Google Scholar 

  • Kim JS, Yoon TJ, Yu KN, Kim BG, Park SJ, Kim HW, Lee KH, Park SB, Lee JK, Cho MH (2006) Toxicity and tissue distribution of magnetic nanoparticles in mice. Toxicol Sci 89(1):338–347

    Article  CAS  Google Scholar 

  • Kim YS, Kim JS, Cho HS, Rha DS, Kim JM, Park JD, Choi BS, Lim R, Chang HK, Chung YH, Kwon IH, Jeong J, Han BS, Yu IJ (2008) Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague–Dawley rats. Inhal Toxicol 20(6):575–583

    Article  CAS  Google Scholar 

  • Klemm D, Schumann D, Udhardt U, Marsch S (2001) Bacterial synthesized cellulose—artificial blood vessels for microsurgery. Prog Polym Sci 26(9):1561–1603

    Article  CAS  Google Scholar 

  • Kotel’nikova NE, Demidov VN, Wegener G, Windeisen E (2003) Mechanisms of diffusion-reduction interaction of microcrystalline cellulose and silver ions. Russ J Gen Chem 73(3):427–433

    Article  Google Scholar 

  • Kundu S, Wang K, Liang H (2009) Size-controlled synthesis and self-assembly of silver nanoparticles within a minute using microwave irradiation. J Phys Chem C 113(1):134–141

    Article  CAS  Google Scholar 

  • Lansdown AB, Williams A (2004) How safe is silver in wound care? J Wound Care 13(4):131–136

    Article  CAS  Google Scholar 

  • Liz-Marzán LM (2006) Tailoring surface plasmons through the morphology and assembly of metal nanoparticles. Langmuir 22(1):32–41

    Article  Google Scholar 

  • Maneerung T, Tokura S, Rujiravanit R (2008) Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydr Polym 72(1):43–51

    Article  CAS  Google Scholar 

  • Morones-Ramirez JR, Winkler JA, Spina CS, Collins JJ (2013) Silver enhances antibiotic activity against gram-negative bacteria. Sci Transl Med 5(190):190ra81–190ra81

  • Novaes Júnior AB, Novaes AB (1993) Bone formation over a TiAl6V4 (IMZ) implant placed into an extraction socket in association with membrane therapy (Gengiflex). Clin Oral Implant Res 4(2):106–110

    Article  Google Scholar 

  • Pang M, Hu J, Zeng HC (2010) Synthesis, morphological control, and antibacterial properties of hollow/solid Ag2S/Ag heterodimers. J Am Chem Soc 132(31):10771–10785

    Article  CAS  Google Scholar 

  • Petersen N, Gatenholm P (2011) Bacterial cellulose-based materials and medical devices: current state and perspectives. Appl Microbiol Biotechnol 91(5):1277–1286

    Article  CAS  Google Scholar 

  • Pinkert A, Marsh KN, Pang S, Staiger MP (2009) Ionic liquids and their interaction with cellulose. Chem Rev 109(12):6712–6728

    Article  CAS  Google Scholar 

  • Pinto RJB, Marques PAAP, Neto CP, Trindade T, Daina S, Sadocco P (2009) Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers. Acta Biomater 5(6):2279–2289

    Article  CAS  Google Scholar 

  • Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27(1):76–83

    Article  CAS  Google Scholar 

  • Sanchavanakit N, Sangrungraungroj W, Kaomongkolgit R, Banaprasert T, Pavasant P, Phisalaphong M (2006) Growth of human keratinocytes and fibroblasts on bacterial cellulose film. Biotechnol Prog 22(4):1194–1199

    Article  CAS  Google Scholar 

  • Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145(1–2):83–96

    Article  CAS  Google Scholar 

  • Shoda M, Sugano Y (2005) Recent advances in bacterial cellulose production. Biotechnol Bioprocess Eng 10(1):1–8

    Article  CAS  Google Scholar 

  • Sokolnicki AM, Fisher RJ, Harrah TP, Kaplan DL (2006) Permeability of bacterial cellulose membranes. J Membr Sci 272(1–2):15–27

    Article  CAS  Google Scholar 

  • Stamplecoskie KG, Scaiano JC, Tiwari VS, Anis H (2011) Optimal size of silver nanoparticles for surface-enhanced raman spectroscopy. J Phys Chem C 115(5):1403–1409

    Article  CAS  Google Scholar 

  • Thomas S, McCubbin P (2003) An in vitro analysis of the antimicrobial properties of 10 silver-containing dressings. J Wound Care 12(8):305–308

    Article  CAS  Google Scholar 

  • Wang Z, Chen X, Chen M, Wu L (2009) Facile fabrication method and characterization of hollow Ag/SiO2 double-shelled spheres. Langmuir 25(13):7646–7651

    Article  CAS  Google Scholar 

  • Wang J, Zhu Y, Du J (2011) Bacterial cellulose: a natural nanomaterial for biomedical applications. J Mech Med Biol 11(2):285–306

    Article  Google Scholar 

  • Watanabe K, Tabuchi M, Morinage Y (1998) Structural features and properties of bacterial cellulose produced in agitated culture. Cellulose 5(3):187–200

    Article  CAS  Google Scholar 

  • Wu J, Zheng Y, Song W, Luan J, Wen X, Wu Z, Chen X, Wang Q, Guo S (2014) In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing. Carbohydr Polym 102(1):762–771

    Article  CAS  Google Scholar 

  • Yang Y, Matsubara S, Xiong L, Hayakawa T, Nogami M (2007) Solvothermal synthesis of multiple shapes of silver nanoparticles and their SERS properties. J Phys Chem C 111(26):9095–9104

    Article  CAS  Google Scholar 

  • Yang G, Xie J, Deng Y, Bian Y, Hong F (2012) Hydrothermal synthesis of bacterial cellulose/AgNPs composite: a “green” route for antibacterial application. Carbohydr Polym 87(4):2482–2487

    Article  CAS  Google Scholar 

  • Yang J, Lv X, Chen S, Li Z, Feng C, Wang H, Xu Y (2014) In situ fabrication of a microporous bacterial cellulose/potato starch composite scaffold with enhanced cell compatibility. Cellulose 21(3):1823–1835

  • Zaborowska M, Bodin A, Bäckdahl H, Popp J, Goldstein A, Gatenholm P (2010) Microporous bacterial cellulose as a potential scaffold for bone regeneration. Acta Biomater 6(7):2540–2547

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Fundamental Research Funds for the Central Universities (2232013D3-01) and the National Natural Science Foundation of China (51273043, 81370795, and 81100488).

Author information

Authors and Affiliations

  1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Key Laboratory of Textile Science and Technology (Ministry of Education), College of Materials Science and Engineering, Donghua University, Shanghai, 201620, People’s Republic of China

    Zhe Li, Lei Wang, Shiyan Chen, Na Yin, Jingxuan Yang & Huaping Wang

  2. School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, People’s Republic of China

    Si Chen

  3. Department of Urology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai, 201620, People’s Republic of China

    Chao Feng & Yuemin Xu

Authors
  1. Zhe Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Si Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chao Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shiyan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Na Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jingxuan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Huaping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yuemin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Shiyan Chen or Huaping Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, Z., Wang, L., Chen, S. et al. Facilely green synthesis of silver nanoparticles into bacterial cellulose. Cellulose 22, 373–383 (2015). https://doi.org/10.1007/s10570-014-0487-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10570-014-0487-9

Keywords

Search

Navigation