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Wound debridement and antibiofilm properties of gamma-ray DMAEMA-grafted onto cotton gauzes

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Abstract

Cotton gauze fabric was functionalized with 2-(dimethylamino)ethyl methacrylate (DMAEMA) with the aim of developing wound dressings with antibiofilm activity and tunable debriding activity. Cotton-g-DMAEMA gauzes were prepared via one-step grafting (direct method) using 60Co γ-rays as source to initiate the polymerization process. The effects of absorbed dose, dose rate, and monomer concentration on the degree of grafting were evaluated in detail. Some cotton-g-DMAEMA gauzes were subsequently quaternized with methyl iodide. Grafting of DMAEMA and sequential quaternization were confirmed by FTIR-ATR spectroscopy; thermal properties were analyzed using TGA, and morphology by scanning electron microscopy. Grafting of DMAEMA gauzes enhanced blood absorption and collagenase activity, while further quaternization led to a remarkable inhibition of the proteinase activity. Their antimicrobial features were analyzed by evaluating their biofilm inhibiting and biofilm eradicating properties in an in vitro chronic wound model. Although the non-quaternized gauzes only displayed moderate biofilm inhibitory properties at best, the quaternized cotton-g-DMAEMA bearing the highest content of DMAEMA displayed strong biofilm inhibiting and biofilm eradicating properties. This indicates that quaternized cotton-g-DMAEMA gauzes are less prone to be colonized by bacteria and can notably reduce the number of colonies in an infected wound.

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Acknowledgments

The authors thank to M. Cruz, F. García and B. Leal from ICN-UNAM for technical assistance. This work was supported by DGAPA-UNAM Grant IN200714 and CONACYT-CNPq Project 174378 Mexico, MICINN (SAF2011-22771) Spain and FEDER. Authors also thank “Red iberoamericana de nuevos materiales para el diseño de sistemas avanzados de liberación de fármacos en enfermedades de alto impacto socioeconómico” (RIMADEL) of the Ibero-American Programme for Science, Technology and Development (CYTED) and funding by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen, SBO programme).

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Authors and Affiliations

  1. Departamento de Química de Radiaciones y Radioquímica, Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, 04510, Mexico, D.F., Mexico

    Marco A. Luna-Straffon & Emilio Bucio

  2. Laboratorio de Investigación y Desarrollo, Signa S.A. de C.V., Av. Industrial Automotriz 301, Zona Industrial, 50071, Toluca, Estado de México, Mexico

    Angel Contreras-García

  3. Laboratory of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Gilles Brackman & Tom Coenye

  4. Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    Angel Concheiro & Carmen Alvarez-Lorenzo

Authors
  1. Marco A. Luna-Straffon
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  2. Angel Contreras-García
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  3. Gilles Brackman
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  4. Tom Coenye
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  5. Angel Concheiro
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  6. Carmen Alvarez-Lorenzo
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  7. Emilio Bucio
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Correspondence to Emilio Bucio.

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Luna-Straffon, M.A., Contreras-García, A., Brackman, G. et al. Wound debridement and antibiofilm properties of gamma-ray DMAEMA-grafted onto cotton gauzes. Cellulose 21, 3767–3779 (2014). https://doi.org/10.1007/s10570-014-0371-7

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  • DOI: https://doi.org/10.1007/s10570-014-0371-7

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