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Nanocrystalline cellulose/β-casein conjugated nanoparticles prepared by click chemistry

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Abstract

Functionalized nanoparticles are promising building blocks for well-defined nanomaterials with unique properties. Site-specific or regio-selective functionalization of those nanoparticles and organization into high-order assemblies is a major challenge in materials research. Here, we demonstrate site-specific immobilization of a model protein at one tip of nanocrystalline cellulose (NCC), single-crystalline rod-like shaped nanoparticles that are isolated by acid hydrolysis of bulk cellulose. Click reaction between reducing end functionalized NCC bearing azide groups and β-casein micelles bearing acetylene groups results in mushroom-like conjugated nanoparticles in different arrangements. The strategy developed here to design hybrid polysaccharide–protein nanoparticles could be useful for building novel functional self-assembled nanobiomaterials and have potential in nanomedicine, immunoassay and drug delivery applications.

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Acknowledgments

This work was funded by a Natural Sciences and Engineering Research Council of Canada (NSERC) Strategic Project Grant.

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Authors and Affiliations

  1. Advanced Biomaterials Chemistry Lab, Faculty of Forestry, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Muzaffer A. Karaaslan, Guangzheng Gao & John F. Kadla

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  1. Muzaffer A. Karaaslan
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  2. Guangzheng Gao
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  3. John F. Kadla
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Correspondence to John F. Kadla.

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Karaaslan, M.A., Gao, G. & Kadla, J.F. Nanocrystalline cellulose/β-casein conjugated nanoparticles prepared by click chemistry. Cellulose 20, 2655–2665 (2013). https://doi.org/10.1007/s10570-013-0065-6

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