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The root of Actinidia chinensis inhibits hepatocellular carcinomas cells through LAMB3

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Abstract

The root of Actinidia chinensis, as traditional Chinese medicine, has been shown to inhibit cell proliferation in numerous cancer cells. However, the mechanisms underlying its inhibitory activity remain unclear. Death rates of hepatocellular carcinoma (HCC) are increasing, but therapies for advanced HCC are not well developed. We choose the extract from root of Actinidia chinensis (ERAC) to treat the HCC cell lines in vitro, displaying distinct effects on cell proliferation, S-phase cell cycle arrest, and apoptosis. LAMB3, the gene encoding laminin subunit beta-3, plays a key role in the proliferation suppression and S-phase cell cycle arrest of HepG2 cells treated with ERAC. The downstream genes ITGA3, CCND2, and TP53 in LAMB3 pathway show the same response to ERAC as LAMB3. Thus, LAMB3 pathways, along with extracellular matrix-receptor interaction, pathways in cancer, and focal adhesion, are involved in the ERAC-induced suppressive response in HepG2.

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Authors and Affiliations

  1. Zhongshan Hospital Institute of Clinical Science, Shanghai Institute of Clinical Bioinformatics; Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Jiayun Hou, Lingyan Wang & Duojiao Wu

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  1. Jiayun Hou
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  2. Lingyan Wang
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  3. Duojiao Wu
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Correspondence to Duojiao Wu.

Electronic supplementary material

Supplemental Figure 1

A, quality control of microarray experiments. B, the expression profile of genes were shown as 0.2 fold change per box in the screen for ERAC action genes of LM3. C, the expression profile of genes were shown as 0.2 fold change per box in the screen for ERAC action genes of HepG2. (PDF 62.5 kb)

Supplemental Figure 2

LAMB3 involved pathways analyses of ERAC action. A, Quantitation of important laminin isoforms of ERAC action. B, Quantitation of important genes in LAMB3 involved pathways. (PDF 204 kb)

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Hou, J., Wang, L. & Wu, D. The root of Actinidia chinensis inhibits hepatocellular carcinomas cells through LAMB3. Cell Biol Toxicol 34, 321–332 (2018). https://doi.org/10.1007/s10565-017-9416-7

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  • DOI: https://doi.org/10.1007/s10565-017-9416-7

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