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Cytotoxicity, apoptosis, DNA damage and methylation in mammary and kidney epithelial cell lines exposed to ochratoxin A

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Abstract

This study aimed to investigate the in vitro damage induced by ochratoxin A (OTA) in BME-UV1 and MDCK epithelial cells. Both cells lines were treated with OTA (0 up to 10 μg/mL), and cell viability (MTT assay), membrane stability (lactate dehydrogenase (LDH) release assay) and apoptotic cell rate (Tunel assay) were investigated. Further, the effect of the incubation with OTA has been evaluated at DNA level by the determination of DNA integrity, by the quantification of DNA adduct formation (8-hydroxy-2′-deoxyguanosine (8-OHdG)) and by the assessment of the global DNA methylation status (5-methyl-cytosine (5-mC)). The obtained results showed that after 24 h of OTA treatment, BME-UV1 cell viability was reduced in a dose-dependent way. OTA significantly (P < 0.05) increased LDH release in BME-UV1 cells at all concentrations tested. OTA (1.25 μg/mL) induced 35 % LDH release in MDCK cells (P < 0.05). A significant (P < 0.05) change in percentages of apoptotic BME-UV1 (10 ± 0.86) and MDCK (25 ± 0.88) cells was calculated when the cells were co-incubated with OTA. The level of 8-OHdG adduct formation was significantly (P < 0.05) increased in BME-UV1 cells treated with 1.25 μg/mL of OTA. The results of the present study suggest that a different mechanism of action may occur in these cell lines.

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Authors and Affiliations

  1. Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Via D. Trentacoste 2, 20134, Milan, Italy

    Carlotta Giromini, Raffaella Rebucci, Eleonora Fusi, Luciana Rossi, Francesca Saccone & Antonella Baldi

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  1. Carlotta Giromini
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  2. Raffaella Rebucci
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  3. Eleonora Fusi
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  4. Luciana Rossi
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  5. Francesca Saccone
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  6. Antonella Baldi
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Correspondence to Carlotta Giromini.

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Giromini, C., Rebucci, R., Fusi, E. et al. Cytotoxicity, apoptosis, DNA damage and methylation in mammary and kidney epithelial cell lines exposed to ochratoxin A. Cell Biol Toxicol 32, 249–258 (2016). https://doi.org/10.1007/s10565-016-9332-2

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  • DOI: https://doi.org/10.1007/s10565-016-9332-2

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