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Fenugreek seed (Trigonella foenum graecum) polyphenols inhibit ethanol-induced collagen and lipid accumulation in rat liver

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Abstract

Chronic alcoholism is associated with fatty liver and fibrosis characterized by collagen accumulation. Seeds of fenugreek, an annual herb, are reported to possess hepatoprotective activity. The study aims to investigate the effects of fenugreek seed polyphenol extract (FPEt) on liver lipids and collagen in experimental hepatotoxic rats. Hepatotoxicity was induced in male albino Wistar rats by administrating ethanol (6 g/kg per day) for 30 days. Control rats were given isocaloric glucose solution. FPEt was co-administered with ethanol at a dose of 200 mg/kg per day for the next 30 days. Silymarin was used as a positive control. Ethanol treatment caused increase in plasma and liver lipids, together with alterations in collagen content and properties. Administration of FPEt to alcohol-fed rats significantly improved lipid profile and reduced collagen content, crosslinking, aldehyde content and peroxidation. The effects were comparable with that of silymarin. FPEt administration had a positive influence on both lipid profile and on the quantitative and qualitative properties of collagen in alcoholic liver disease. The protective effect is presumably due to the bioactive phytochemicals in fenugreek seeds.

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Abbreviations

DMRT:

Duncan’s multiple range test

FFA:

free fatty acids

FPEt:

fenugreek seed polyphenol extract

H&E:

hematoxylin and eosin

HDL-C:

high-density lipoprotein cholesterol

LDL-C:

low-density lipoprotein cholesterol

PL:

phospholipids

TG:

triglycerides

VLDL-C:

very low-density lipoprotein cholesterol

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu, 608 002, India

    S. Kaviarasan & C. V. Anuradha

  2. Department of Pathology, Faculty of Medicine, RMMC&H, Annamalai University, Annamalai Nagar, Tamil Nadu, 608 002, India

    P. Viswanathan

Authors
  1. S. Kaviarasan
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  2. P. Viswanathan
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  3. C. V. Anuradha
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Correspondence to C. V. Anuradha.

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Kaviarasan, S., Viswanathan, P. & Anuradha, C.V. Fenugreek seed (Trigonella foenum graecum) polyphenols inhibit ethanol-induced collagen and lipid accumulation in rat liver. Cell Biol Toxicol 23, 373–383 (2007). https://doi.org/10.1007/s10565-007-9000-7

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