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Repairing cartilage defects with bone marrow mesenchymal stem cells induced by CDMP and TGF-β1

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Abstract

The aim of this study was to explore the ability for chondrogenic differentiation of bone marrow mesenchymal stems cells (BMSCs) induced by either cartilage-derived morphogenetic protein 1 (CDMP-1) alone or in the presence of transforming growth factor-β1 (TGF-β1) in vivo and in vitro. BMSCs and poly-lactic acid/glycolic acid copolymer (PLGA) scaffold were analyzed for chondrogenic capacity induced by CDMP-1 and TGF-β1 in vivo and in vitro. Chondrogenic differentiation of BMSCs into chondrocytes using a high density pellet culture system was tested, whether they could be maintained in 3-D PLGA scaffold instead of pellet culture remains to be explored. Under the culture of high-density cell suspension and PLGA frame, BMSCs were observed the ability to repair cartilage defects by either CDMP-1 alone or in the presence of TGF-β1 in vitro. Then the cell-scaffold complex was implanted into animals for 4 and 8 weeks for in vivo test. The content of collagen type II and proteoglycan appeared to increase over time in the constructs of the induced groups (CDMP in the presence of TGF-β1), CDMP group and TGF group. However, the construct of the control group did not express them during the whole culture time. At 4 and 8 weeks, the collagen type II expression of the induced group was higher than the sum of TGF group and CDMP group by SSPS17.0 analysis. BMSCs and PLGA complex induced by CDMP-1 and TGF- β1 can repair cartilage defects more effectively than that induced by CDMP-1 or TGF-β1 only.

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Acknowledgments

This study was supported by the Grant of Education Department of Liaoning Province (No: LT2010063, 2010T110) and Science and Technology Department of Liaoning Province (No: 20062198).

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Authors and Affiliations

  1. Department of Otolaryngology, The First Affiliated Hospital of Liaoning Medical College, Jinzhou City, 121001, Liaoning Province, China

    Ying Cui, Xinyu Pan, Xuefeng Wang & Ben Zhang

  2. Department of Surgery, The First Affiliated Hospital of Liaoning Medical College, Jinzhou City, 121001, Liaoning Province, China

    Gang Wu

  3. Department of Otolaryngology, The Affiliated Hospital of Jining Medical College, Jining City, 272029, Shandong Province, China

    Linxiang Ma

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  1. Gang Wu
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  2. Ying Cui
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  3. Linxiang Ma
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  4. Xinyu Pan
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  5. Xuefeng Wang
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  6. Ben Zhang
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Correspondence to Ying Cui.

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Wu, G., Cui, Y., Ma, L. et al. Repairing cartilage defects with bone marrow mesenchymal stem cells induced by CDMP and TGF-β1 . Cell Tissue Bank 15, 51–57 (2014). https://doi.org/10.1007/s10561-013-9369-x

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  • DOI: https://doi.org/10.1007/s10561-013-9369-x

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